Anti-Rootletin antibody

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

Rootletin also known as C-Nap1-interacting protein or CEP68 is a large coiled-coil protein with a mass around 230 kDa. It is primarily expressed in centrosomes a major microtubule-organizing center in cells. Rootletin forms the structural rootlet in ciliated cells supporting the cilia as microtubules extend outward. It serves a structural role in maintaining centriole cohesion a necessary process for centrosome stability and proper cellular division.

Biological function summary

The protein contributes significantly to microtubule organization and affects cell stability. Rootletin is a part of the ciliary rootlet complex influencing centrosome organization and ciliary function. Its presence is important for organizing the dense fibers that extend from the basal bodies which helps in maintaining centriole connections. Rootletin interactions impact centrosome cohesion cellular division and functional operation of cilia which are important for motility and sensory input.

Pathways

Rootletin manages its role in centrosome cohesion impacting processes such as the cell cycle. It is significant in the centrosome duplication pathway working in close relation with proteins like CEP135 and ninein. These interactions ensure centrosome stability influencing key processes like prophase separation. Additionally rootletin can oppose CEP164 and other distal appendage proteins maintaining centriole cohesion and preventing premature disengagement thereby maintaining the intricate balance required for appropriate cell function and division.

Associated diseases and disorders

Rootletin is involved in disorders related to ciliary dysfunction and centrosome abnormalities. Disruptions in rootletin's function can contribute to ciliopathies like Bardet-Biedl syndrome causing defects due to improper cilia formation and action. It interacts with proteins like kinesin family member 3A (KIF3A) which when malfunctioning can lead to abnormal cellular transport and related disorders. Rootletin abnormalities have also been implicated in certain cancers as centrosome amplification can lead to aneuploidy and subsequent tumorigenesis. Understanding its role and interactions gives insight into therapeutic opportunities for these conditions.