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AB121653

Anti-Rootletin antibody

5

(1 Review)

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(3 Publications)

Rabbit Polyclonal Rootletin antibody. Suitable for IHC-P, ICC, WB and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human CROCC aa 400-500.

View Alternative Names

KIAA0445, CROCC, Rootletin, Ciliary rootlet coiled-coil protein

6 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rootletin antibody (AB121653)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rootletin antibody (AB121653)

Immunohistochemical analysis of human testis tissue labeling Rootletin with ab121653 at 1/50 dilution.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rootletin antibody (AB121653)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rootletin antibody (AB121653)

Immunohistochemical analysis of human liver tissue labeling Rootletin with ab121653 at 1/50 dilution.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rootletin antibody (AB121653)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rootletin antibody (AB121653)

ab121653, at 1/150 dilution, staining Rootletin in Human fallopian tube tissue by Immunohistochemistry using formalin-fixed, paraffin-embedded tissue.

Immunocytochemistry - Anti-Rootletin antibody (AB121653)
  • ICC

Supplier Data

Immunocytochemistry - Anti-Rootletin antibody (AB121653)

Immunocytochemistry analysis of U-2 OS (human bone osteosarcoma epithelial cell line) cells labelling Rootletin with ab121653 at 2 μg/mL.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rootletin antibody (AB121653)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rootletin antibody (AB121653)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human bronchus tissue labelling Rootletin with ab121653 at 1/50 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.

Western blot - Anti-Rootletin antibody (AB121653)
  • WB

Supplier Data

Western blot - Anti-Rootletin antibody (AB121653)

All lanes:

Western blot - Anti-Rootletin antibody (ab121653) at 0.4 µg/mL

All lanes:

SCLC-21H (human small cell lung carcinoma cell line) cell lysate

Predicted band size: 229 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ICC, IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human CROCC aa 400-500. The exact immunogen used to generate this antibody is proprietary information.

Q5TZA2

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICC" : {"fullname" : "Immunocytochemistry", "shortname":"ICC"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/200", "IHCP-species-notes": "<p>Use buffer at pH 6 for antigen retrieval.</p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "ICC-species-checked": "testedAndGuaranteed", "ICC-species-dilution-info": "0.25-2 µg/mL", "ICC-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Rootletin also known as C-Nap1-interacting protein or CEP68 is a large coiled-coil protein with a mass around 230 kDa. It is primarily expressed in centrosomes a major microtubule-organizing center in cells. Rootletin forms the structural rootlet in ciliated cells supporting the cilia as microtubules extend outward. It serves a structural role in maintaining centriole cohesion a necessary process for centrosome stability and proper cellular division.
Biological function summary

The protein contributes significantly to microtubule organization and affects cell stability. Rootletin is a part of the ciliary rootlet complex influencing centrosome organization and ciliary function. Its presence is important for organizing the dense fibers that extend from the basal bodies which helps in maintaining centriole connections. Rootletin interactions impact centrosome cohesion cellular division and functional operation of cilia which are important for motility and sensory input.

Pathways

Rootletin manages its role in centrosome cohesion impacting processes such as the cell cycle. It is significant in the centrosome duplication pathway working in close relation with proteins like CEP135 and ninein. These interactions ensure centrosome stability influencing key processes like prophase separation. Additionally rootletin can oppose CEP164 and other distal appendage proteins maintaining centriole cohesion and preventing premature disengagement thereby maintaining the intricate balance required for appropriate cell function and division.

Rootletin is involved in disorders related to ciliary dysfunction and centrosome abnormalities. Disruptions in rootletin's function can contribute to ciliopathies like Bardet-Biedl syndrome causing defects due to improper cilia formation and action. It interacts with proteins like kinesin family member 3A (KIF3A) which when malfunctioning can lead to abnormal cellular transport and related disorders. Rootletin abnormalities have also been implicated in certain cancers as centrosome amplification can lead to aneuploidy and subsequent tumorigenesis. Understanding its role and interactions gives insight into therapeutic opportunities for these conditions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Major structural component of the ciliary rootlet, a cytoskeletal-like structure in ciliated cells which originates from the basal body at the proximal end of a cilium and extends proximally toward the cell nucleus (By similarity). Furthermore, is required for the correct positioning of the cilium basal body relative to the cell nucleus, to allow for ciliogenesis (PubMed : 27623382). Contributes to centrosome cohesion before mitosis (PubMed : 16203858).
See full target information CROCC
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Neuron 111:65-80.e6 PubMed36334595

2022

CROCCP2 acts as a human-specific modifier of cilia dynamics and mTOR signaling to promote expansion of cortical progenitors.

Applications

Unspecified application

Species

Unspecified reactive species

Roxane Van Heurck,Jérôme Bonnefont,Marta Wojno,Ikuo K Suzuki,Fausto D Velez-Bravo,Emir Erkol,Dan Truc Nguyen,Adèle Herpoel,Angéline Bilheu,Sofie Beckers,Catherine Ledent,Pierre Vanderhaeghen

Cell stem cell 27:920-936.e8 PubMed33147489

2020

Multimerization of Zika Virus-NS5 Causes Ciliopathy and Forces Premature Neurogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Murielle Saade,Diego S Ferrero,José Blanco-Ameijeiras,Elena Gonzalez-Gobartt,Marco Flores-Mendez,Victor M Ruiz-Arroyo,Elena Martínez-Sáez,Santiago Ramón Y Cajal,Naiara Akizu,Nuria Verdaguer,Elisa Martí

FASEB journal : official publication of the Federa 33:8892-8904 PubMed31051095

2019

Two pools of IRE1α in cardiac and skeletal muscle cells.

Applications

Unspecified application

Species

Unspecified reactive species

Qian Wang,Jody Groenendyk,Tautvydas Paskevicius,Wenying Qin,Kaylen C Kor,Yingjie Liu,Florian Hiess,Bjorn C Knollmann,S R Wayne Chen,Jingfeng Tang,Xing-Zhen Chen,Luis B Agellon,Marek Michalak
View all publications
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