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AB241406

Anti-Roquin-2 antibody

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(2 Publications)

Rabbit Polyclonal Roquin-2 antibody. Suitable for IP and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human RC3H2 aa 900-950.

View Alternative Names

MNAB, RNF164, RC3H2, Roquin-2, Membrane-associated nucleic acid-binding protein, RING finger and CCCH-type zinc finger domain-containing protein 2, RING finger protein 164, RING-type E3 ubiquitin transferase Roquin-2

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Immunoprecipitation - Anti-Roquin-2 antibody (AB241406)
  • IP

Supplier Data

Immunoprecipitation - Anti-Roquin-2 antibody (AB241406)

Roquin-2 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) using ab241406 at 6 μg per reaction. Western blot was performed on the immunoprecipitate using ab241406 at 0.04 μg/ml.

Lane 1 : ab241406 IP in HeLa whole cell lysate.
Lane 2 : Control IgG in HeLa whole cell lysate.

Detection : Chemiluminescence with an exposure time of 3 minutes.

Lysates prepared using NETN lysis buffer.

All lanes:

Immunoprecipitation - Anti-Roquin-2 antibody (ab241406)

Predicted band size: 131 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IP

applications

Immunogen

Synthetic Peptide within Human RC3H2 aa 900-950. The exact immunogen used to generate this antibody is proprietary information.

Q9HBD1

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-10 µg/mg of lysate", "IP-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "" } } }
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Product details

ab241406 has not performed satisfactorily when used for WB of Roquin-2 in crude preparations (e.g. whole cell lysate). This antibody can be used for WB of enriched (e.g. immunoprecipitated) sources of Roquin-2.
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
ab241406 was affinity purified using an epitope specific to Roquin-2 immobilized on solid support.
Storage buffer
pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: Tris citrate/phosphate
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Roquin-2 also known as RC3H2 is a protein recognized for its role in post-transcriptional regulation. The protein has a mass of approximately 125 kilodaltons. You can find Roquin-2 primarily expressed in immune cells such as T-cells and B-cells but it is also present in other tissues. It contains a RING finger domain that allows it to function as an E3 ubiquitin ligase. This domain plays an important role in protein-protein interactions and tagging other proteins for degradation.
Biological function summary

Roquin-2 regulates the stability and translation of several mRNAs involved in inflammatory responses. Acting as a post-transcriptional modulator Roquin-2 binds to specific sequences in the 3' untranslated region (UTR) of target mRNAs affecting their degradation or translation. Roquin-2 is part of a larger complex that includes other regulatory proteins allowing for precise control over mRNA fate. This control impacts various immune functions which is vital for maintaining immune homeostasis.

Pathways

Research has shown that Roquin-2 integrates into the immune response pathways. It has significant involvement in the NF-κB signaling pathway where it modulates the production of cytokines. Roquin-2 also interacts with the TTP protein which is another post-transcriptional regulator highlighting its role in inflammation-related pathways. These interactions influence gene expression patterns that are necessary for appropriate immune functions.

Current studies indicate Roquin-2 has implications in autoimmune diseases such as systemic lupus erythematosus. Alterations in Roquin-2 function can lead to dysregulated immune responses contributing to such disorders. Roquin-2 is connected to proteins involved in autoimmune pathways including ICOS which plays a role in T-cell activation. Understanding how Roquin-2 controls these pathways could offer insights into therapeutic approaches for autoimmune conditions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Post-transcriptional repressor of mRNAs containing a conserved stem loop motif, called constitutive decay element (CDE), which is often located in the 3'-UTR, as in HMGXB3, ICOS, IER3, NFKBID, NFKBIZ, PPP1R10, TNF and in many more mRNAs. Binds to CDE and promotes mRNA deadenylation and degradation. This process does not involve miRNAs. In follicular helper T (Tfh) cells, represses of ICOS and TNFRSF4 expression, thus preventing spontaneous Tfh cell differentiation, germinal center B-cell differentiation in the absence of immunization and autoimmunity. In resting or LPS-stimulated macrophages, controls inflammation by suppressing TNF expression. Also recognizes CDE in its own mRNA and in that of paralogous RC3H1, possibly leading to feedback loop regulation (By similarity). miRNA-binding protein that regulates microRNA homeostasis. Enhances DICER-mediated processing of pre-MIR146a but reduces mature MIR146a levels through an increase of 3' end uridylation. Both inhibits ICOS mRNA expression and they may act together to exert the suppression (PubMed : 25697406). Acts as a ubiquitin E3 ligase. Pairs with E2 enzymes UBE2B, UBE2D2, UBE2E2, UBE2E3, UBE2G2, UBE2K and UBE2Q2 and produces polyubiquitin chains (PubMed : 26489670). Shows the strongest activity when paired with UBE2N : UBE2V1 or UBE2N : UBE2V2 E2 complexes and generate both short and long polyubiquitin chains (PubMed : 26489670). Involved in the ubiquitination of MAP3K5 (PubMed : 24448648, PubMed : 26489670, PubMed : 29186683). Able to interact with double-stranded RNA (dsRNA) (PubMed : 26489670).
See full target information RC3H2
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Molecular medicine reports 28: PubMed37326104

2023

Construction of ceRNA regulatory networks for osteoporosis.

Applications

Unspecified application

Species

Unspecified reactive species

Hongtao Chen,Hailong Wang,Xu Liu,Long Li,Yiliyaer Abudusimu,Yilihamu Tuoheti

Journal of experimental & clinical cancer research : CR 41:17 PubMed34998399

2022

The RNA-binding protein GRSF1 promotes hepatocarcinogenesis via competitively binding to YY1 mRNA with miR-30e-5p.

Applications

Unspecified application

Species

Unspecified reactive species

Lili Han,Chen Huang,Xiaofei Wang,Dongdong Tong
View all publications
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