JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB250935

Anti-ROR beta/RORB antibody [EPR15552] - BSA and Azide free

Be the first to review this product! Submit a review

|

(2 Publications)

Rabbit Recombinant Monoclonal ROR beta/RORB antibody. Carrier free. Suitable for ICC/IF, WB and reacts with Human samples. Cited in 2 publications.

View Alternative Names

NR1F2, RZRB, RORB, Nuclear receptor ROR-beta, Nuclear receptor RZR-beta, Nuclear receptor subfamily 1 group F member 2, Retinoid-related orphan receptor-beta

3 Images
Immunocytochemistry/ Immunofluorescence - Anti-ROR beta/RORB antibody [EPR15552] - BSA and Azide free (AB250935)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-ROR beta/RORB antibody [EPR15552] - BSA and Azide free (AB250935)

Immunofluorescent analysis of 100% methanol-fixed, 0.1% TritonX-100 permeabilized HepG2 (human hepatocellular carcinoma epithelial cell) labelling ROR beta/RORB with ab187657 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488 (ab150077) secondary antibody at 1/1000 dilution (green).

Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) at 1/200 dilution was used as a counterstain (red).

The nuclear counterstain is DAPI (blue).

Confocal image showing nuclear staining in HepG2 cell line. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab187657).

Western blot - Anti-ROR beta/RORB antibody [EPR15552] - BSA and Azide free (AB250935)
  • WB

Supplier Data

Western blot - Anti-ROR beta/RORB antibody [EPR15552] - BSA and Azide free (AB250935)

This data was developed using ab187657, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-ROR beta/RORB antibody [EPR15552] - N-terminal (<a href='/en-us/products/primary-antibodies/ror-beta-rorb-antibody-epr15552-n-terminal-ab187657'>ab187657</a>) at 1/1000 dilution

All lanes:

HepG2 cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 53 kDa

false

Western blot - Anti-ROR beta/RORB antibody [EPR15552] - BSA and Azide free (AB250935)
  • WB

Supplier Data

Western blot - Anti-ROR beta/RORB antibody [EPR15552] - BSA and Azide free (AB250935)

This data was developed using ab187657, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-ROR beta/RORB antibody [EPR15552] - N-terminal (<a href='/en-us/products/primary-antibodies/ror-beta-rorb-antibody-epr15552-n-terminal-ab187657'>ab187657</a>) at 1/1000 dilution

All lanes:

Human cerebellum tissue lysate at 10 µg

Secondary

All lanes:

Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 33 kDa,53 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR15552

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

ICC/IF, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" }, "Mouse": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" } } }
style
left-column

Product details

ab250935 is the carrier-free version of ab187657.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
style
left-column
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Nuclear receptor that binds DNA as a monomer to ROR response elements (RORE) containing a single core motif half-site 5'-AGGTCA-3' preceded by a short A-T-rich sequence. Considered to have intrinsic transcriptional activity, have some natural ligands such as all-trans retinoic acid (ATRA) and other retinoids which act as inverse agonists repressing the transcriptional activity. Required for normal postnatal development of rod and cone photoreceptor cells. Modulates rod photoreceptors differentiation at least by inducing the transcription factor NRL-mediated pathway. In cone photoreceptor cells, regulates transcription of OPN1SW. Involved in the regulation of the period length and stability of the circadian rhythm. May control cytoarchitectural patterning of neocortical neurons during development. May act in a dose-dependent manner to regulate barrel formation upon innervation of layer IV neurons by thalamocortical axons. May play a role in the suppression of osteoblastic differentiation through the inhibition of RUNX2 transcriptional activity (By similarity).. Isoform 1 is critical for hindlimb motor control and for the differentiation of amacrine and horizontal cells in the retina. Regulates the expression of PTF1A synergistically with FOXN4 (By similarity).
See full target information RORB
style
left-column

Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 14:3728 PubMed37349339

2023

NBEAL2 deficiency in humans leads to low CTLA-4 expression in activated conventional T cells.

Applications

Unspecified application

Species

Unspecified reactive species

Laure Delage,Francesco Carbone,Quentin Riller,Jean-Luc Zachayus,Erwan Kerbellec,Armelle Buzy,Marie-Claude Stolzenberg,Marine Luka,Camille de Cevins,Georges Kalouche,Rémi Favier,Alizée Michel,Sonia Meynier,Aurélien Corneau,Caroline Evrard,Nathalie Neveux,Sébastien Roudières,Brieuc P Pérot,Mathieu Fusaro,Christelle Lenoir,Olivier Pellé,Mélanie Parisot,Marc Bras,Sébastien Héritier,Guy Leverger,Anne-Sophie Korganow,Capucine Picard,Sylvain Latour,Bénédicte Collet,Alain Fischer,Bénédicte Neven,Aude Magérus,Mickaël Ménager,Benoit Pasquier,Frédéric Rieux-Laucat

Endocrine-related cancer 25:35-50 PubMed29042395

2017

Nuclear receptor profiling in prostatospheroids and castration-resistant prostate cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Zhu Wang,Dinglan Wu,Chi-Fai Ng,Jeremy Yuen-Chun Teoh,Shan Yu,Yuliang Wang,Franky L Chan
View all publications
style
left-column
style
left-column
style
right-column

Complementary Products

Primary Antibodies

AB15580

Anti-Ki67 antibody

BOND RX™ Validated|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ki67 antibody (AB15580)

  • 5
  • 230
Primary Antibodies

AB113434

Anti-ROR gamma antibody

Flow Cytometry - Anti-ROR gamma antibody (AB113434)

  • 4
  • 1
Primary Antibodies

AB31940

Anti-TBR1 antibody

BOND RX™ Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TBR1 antibody (AB31940)

  • 4
  • 32
Primary Antibodies

AB97959

Anti-SOX2 antibody

BOND RX™ Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX2 antibody (AB97959)

  • 4
  • 75

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com