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AB187657

Anti-ROR beta/RORB antibody [EPR15552] - N-terminal

4

(1 Review)

|

(1 Publication)

Rabbit Recombinant Monoclonal ROR beta/RORB antibody. N-terminal. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 1 publication.

View Alternative Names

NR1F2, RZRB, RORB, Nuclear receptor ROR-beta, Nuclear receptor RZR-beta, Nuclear receptor subfamily 1 group F member 2, Retinoid-related orphan receptor-beta

3 Images
Immunocytochemistry/ Immunofluorescence - Anti-ROR beta/RORB antibody [EPR15552] - N-terminal (AB187657)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-ROR beta/RORB antibody [EPR15552] - N-terminal (AB187657)

Immunofluorescent analysis of 100% methanol-fixed, 0.1% TritonX-100 permeabilized HepG2 (human hepatocellular carcinoma epithelial cell) labelling ROR beta/RORB with ab187657 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488 (ab150077) secondary antibody at 1/1000 dilution (green).

Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) at 1/200 dilution was used as a counterstain (red).

The nuclear counterstain is DAPI (blue).

Confocal image showing nuclear staining in HepG2 cell line. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Western blot - Anti-ROR beta/RORB antibody [EPR15552] - N-terminal (AB187657)
  • WB

Supplier Data

Western blot - Anti-ROR beta/RORB antibody [EPR15552] - N-terminal (AB187657)

All lanes:

Western blot - Anti-ROR beta/RORB antibody [EPR15552] - N-terminal (ab187657) at 1/1000 dilution

All lanes:

HepG2 cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 53 kDa

false

Western blot - Anti-ROR beta/RORB antibody [EPR15552] - N-terminal (AB187657)
  • WB

Supplier Data

Western blot - Anti-ROR beta/RORB antibody [EPR15552] - N-terminal (AB187657)

All lanes:

Western blot - Anti-ROR beta/RORB antibody [EPR15552] - N-terminal (ab187657) at 1/1000 dilution

All lanes:

Human cerebellum tissue lysate at 10 µg

Secondary

All lanes:

Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 33 kDa,53 kDa

false

  • Carrier free

    Anti-ROR beta/RORB antibody [EPR15552] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR15552

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/2000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/1000", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "1/1000 - 1/2000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "1/1000", "ICCIF-species-notes": "<p></p>" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Nuclear receptor that binds DNA as a monomer to ROR response elements (RORE) containing a single core motif half-site 5'-AGGTCA-3' preceded by a short A-T-rich sequence. Considered to have intrinsic transcriptional activity, have some natural ligands such as all-trans retinoic acid (ATRA) and other retinoids which act as inverse agonists repressing the transcriptional activity. Required for normal postnatal development of rod and cone photoreceptor cells. Modulates rod photoreceptors differentiation at least by inducing the transcription factor NRL-mediated pathway. In cone photoreceptor cells, regulates transcription of OPN1SW. Involved in the regulation of the period length and stability of the circadian rhythm. May control cytoarchitectural patterning of neocortical neurons during development. May act in a dose-dependent manner to regulate barrel formation upon innervation of layer IV neurons by thalamocortical axons. May play a role in the suppression of osteoblastic differentiation through the inhibition of RUNX2 transcriptional activity (By similarity).. Isoform 1 is critical for hindlimb motor control and for the differentiation of amacrine and horizontal cells in the retina. Regulates the expression of PTF1A synergistically with FOXN4 (By similarity).
See full target information RORB
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

iScience 26:108306 PubMed38026209

2023

Preclinical long-term safety of intraspinal transplantation of human dorsal spinal GABA neural progenitor cells.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaolong Zheng,Zhixian Liu,Ziyu He,Jia Xu,YaNan Wang,ChenZi Gong,Ruoying Zhang,Su-Chun Zhang,Hong Chen,Wei Wang
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions
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Associated Products

Select an associated product type
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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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