Anti-ROR beta/RORB antibody [EPR27136-55]

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ROR beta/RORB antibody [EPR27136-55] (AB314650)

Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling ROR beta/RORB with ab314650 at 1/100 (5.11 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Negative control : No staining on human kidney.The section was incubated with ab314650 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ROR beta/RORB antibody [EPR27136-55] (AB314650)

Immunohistochemical analysis of paraffin-embedded Human cerebrum tissue labeling ROR beta/RORB with ab314650 at 1/100 (5.11 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on human cerebrum.The section was incubated with ab314650 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ROR beta/RORB antibody [EPR27136-55] (AB314650)

Immunohistochemical analysis of paraffin-embedded Human retina tissue labeling ROR beta/RORB with ab314650 at 1/100 (5.11 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on human retina.The section was incubated with ab314650 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ROR beta/RORB antibody [EPR27136-55] (AB314650)

Immunohistochemical analysis of paraffin-embedded Rat retina tissue labeling ROR beta/RORB with ab314650 at 1/500 (1.022 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on rat retina.The section was incubated with ab314650 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ROR beta/RORB antibody [EPR27136-55] (AB314650)

Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue labeling ROR beta/RORB with ab314650 at 1/500 (1.022 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on mouse cerebrum (PMID : 34272258).The section was incubated with ab314650 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ROR beta/RORB antibody [EPR27136-55] (AB314650)

Immunohistochemical analysis of paraffin-embedded Rat cerebrum tissue labeling ROR beta/RORB with ab314650 at 1/500 (1.022 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on rat cerebrum.The section was incubated with ab314650 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ROR beta/RORB antibody [EPR27136-55] (AB314650)

Immunohistochemical analysis of paraffin-embedded Rat kidney tissue labeling ROR beta/RORB with ab314650 at 1/500 (1.022 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Negative control : No staining on rat kidney.The section was incubated with ab314650 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ROR beta/RORB antibody [EPR27136-55] (AB314650)

Immunohistochemical analysis of paraffin-embedded Mouse kidney tissue labeling ROR beta/RORB with ab314650 at 1/500 (1.022 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Negative control : No staining on mouse kidney.The section was incubated with ab314650 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ROR beta/RORB antibody [EPR27136-55] (AB314650)

Immunohistochemical analysis of paraffin-embedded Mouse retina tissue labeling ROR beta/RORB with ab314650 at 1/500 (1.022 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on mouse retina.The section was incubated with ab314650 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• WB

Supplier Data

Western blot - Anti-ROR beta/RORB antibody [EPR27136-55] (AB314650)

Blocking and diluting buffer and concentration : 5% NFDM/TBST The band beneath the target band (53 kDa) is likely to be degraded target fragments. Lysates were freshly made and used for Western blotting immediately to minimize protein degradation.

All lanes:

Western blot - Anti-ROR beta/RORB antibody [EPR27136-55] (ab314650) at 1/1000 dilution

All lanes:

Y79 (human retinoblastoma retinoblastoma) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 40 kDa,53 kDa

false

Exposure time: 125s

• WB

Supplier Data

Western blot - Anti-ROR beta/RORB antibody [EPR27136-55] (AB314650)

Blocking and diluting buffer and concentration : 5% NFDM/TBST Low expression : liver (PMID : 16574740), kidney. In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-ROR beta/RORB antibody [EPR27136-55] (ab314650) at 1/1000 dilution

Lane 1:

Human eyeball tissue lysate at 20 µg

Lane 2:

Human kidney tissue lysate at 20 µg

Lane 3:

Human liver tissue lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Observed band size: 53 kDa

false

Exposure time: 48s

• WB

Supplier Data

Western blot - Anti-ROR beta/RORB antibody [EPR27136-55] (AB314650)

Blocking and diluting buffer and concentration : 5% NFDM/TBST Low expression : liver (PMID : 16574740), kidney. The bands beneath the target band (53 kDa) are likely to be degraded target fragments. In Western blot, Anti-Vinculin antibody [EPR8185] (ab129002) staining at 1/10000 dilution.

All lanes:

Western blot - Anti-ROR beta/RORB antibody [EPR27136-55] (ab314650) at 1/1000 dilution

Lane 1:

Mouse retina tissue lysate at 50 µg

Lane 2:

C57 P20 retina tissue lysate at 50 µg

Lane 3:

Mouse kidney tissue lysate at 50 µg

Lane 4:

Mouse liver tissue lysate at 50 µg

Lane 5:

Rat retina tissue lysate at 50 µg

Lane 6:

Rat kidney tissue lysate at 50 µg

Lane 7:

Rat liver tissue lysate at 50 µg

Secondary

All lanes:

Western blot - VeriBlot for IP Detection Reagent (HRP) (<a href='/en-us/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/1000 dilution

Observed band size: 20 kDa,30 kDa,53 kDa

false

Exposure time: 48s