Anti-ROR beta/RORB antibody [EPR27136-55] - BSA and Azide free (ab314651)

Rabbit Recombinant Monoclonal ROR beta/RORB antibody. Carrier free. Suitable for IHC-P, WB and reacts with Human, Mouse, Rat samples.

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Images

Western blot - Anti-ROR beta/RORB antibody [EPR27136-55] - BSA and Azide free (AB314651), expandable thumbnail

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Constituents: 100% PBS
Form: Liquid
Clonality: Monoclonal

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application

Product promiseTestedExpectedPredictedNot recommended

	IHC-P	WB	ICC/IF	IHC-Fr	Flow Cyt (Intra)	IP
Human	Tested	Tested	Not recommended	Not recommended	Not recommended	Not recommended
Mouse	Tested	Tested	Not recommended	Not recommended	Not recommended	Not recommended
Rat	Tested	Tested	Not recommended	Not recommended	Not recommended	Not recommended

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info -	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species Mouse	Dilution info -	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species Rat	Dilution info -	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Tested
Tested

Species	Dilution info	Notes
Species Human, Mouse, Rat	Dilution info -	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Human, Mouse, Rat	Dilution info -	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Mouse, Human, Rat	Dilution info -	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Human, Mouse, Rat	Dilution info -	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Human, Mouse, Rat	Dilution info -	Notes -

Associated Products

Select an associated product type

1 product for Alternative Version

Target data

See full target information Rorb

Function

Nuclear receptor that binds DNA as a monomer to ROR response elements (RORE) containing a single core motif half-site 5'-AGGTCA-3' preceded by a short A-T-rich sequence. Considered to have intrinsic transcriptional activity, have some natural ligands such as all-trans retinoic acid (ATRA) and other retinoids which act as inverse agonists repressing the transcriptional activity. Required for normal postnatal development of rod and cone photoreceptor cells. Modulates rod photoreceptors differentiation at least by inducing the transcription factor NRL-mediated pathway. In cone photoreceptor cells, regulates transcription of OPN1SW. Involved in the regulation of the period length and stability of the circadian rhythm. May control cytoarchitectural patterning of neocortical neurons during development. May act in a dose-dependent manner to regulate barrel formation upon innervation of layer IV neurons by thalamocortical axons. May play a role in the suppression of osteoblastic differentiation through the inhibition of RUNX2 transcriptional activity. Isoform 1 is critical for hindlimb motor control and for the differentiation of amacrine and horizontal cells in the retina. Regulates the expression of PTF1A synergistically with FOXN4.

Alternative names

Nr1f2, Rorb, Nuclear receptor ROR-beta, Nuclear receptor RZR-beta, Nuclear receptor subfamily 1 group F member 2, Retinoid-related orphan receptor-beta

Recommended products

Rabbit Recombinant Monoclonal ROR beta/RORB antibody. Carrier free. Suitable for IHC-P, WB and reacts with Human, Mouse, Rat samples.

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Constituents: 100% PBS
Form: Liquid
Clonality: Monoclonal
Immunogen: The exact immunogen used to generate this antibody is proprietary information.
Carrier free: Yes
Clone number: EPR27136-55
Purification technique: Affinity purification Protein A
Concentration

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: +4°C

Notes

ab314651 is the carrier-free version of Anti-ROR beta/RORB antibody [EPR27136-55] ab314650.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

12 product images

Western blot - Anti-ROR beta/RORB antibody [EPR27136-55] - BSA and Azide free (ab314651)
This data was developed using Anti-ROR beta/RORB antibody [EPR27136-55] ab314650, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST
Low expression: liver (PMID: 16574740), kidney.

The bands beneath the target band (53 kDa) are likely to be degraded target fragments.
In Western blot, Anti-Vinculin antibody [EPR8185] (Anti-Vinculin antibody [EPR8185] ab129002) staining at 1/10000 dilution.
All lanes: Western blot - Anti-ROR beta/RORB antibody [EPR27136-55] (Anti-ROR beta/RORB antibody [EPR27136-55] ab314650) at 1/1000 dilution
Lane 1: Mouse retina tissue lysate at 50 µg
Lane 2: C57 P20 retina tissue lysate at 50 µg
Lane 3: Mouse kidney tissue lysate at 50 µg
Lane 4: Mouse liver tissue lysate at 50 µg
Lane 5: Rat retina tissue lysate at 50 µg
Lane 6: Rat kidney tissue lysate at 50 µg
Lane 7: Rat liver tissue lysate at 50 µg
Secondary
All lanes: Western blot - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/1000 dilution
Observed band size: 20 kDa, 30 kDa, 53 kDa
Exposure time: 48s
Western blot - Anti-ROR beta/RORB antibody [EPR27136-55] - BSA and Azide free (ab314651)
This data was developed using Anti-ROR beta/RORB antibody [EPR27136-55] ab314650, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST
The band beneath the target band (53 kDa) is likely to be degraded target fragments.

Lysates were freshly made and used for Western blotting immediately to minimize protein degradation.
All lanes: Western blot - Anti-ROR beta/RORB antibody [EPR27136-55] (Anti-ROR beta/RORB antibody [EPR27136-55] ab314650) at 1/1000 dilution
All lanes: Y79 (human retinoblastoma retinoblastoma) whole cell lysate at 20 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Observed band size: 40 kDa, 53 kDa
Exposure time: 125s
Western blot - Anti-ROR beta/RORB antibody [EPR27136-55] - BSA and Azide free (ab314651)
This data was developed using Anti-ROR beta/RORB antibody [EPR27136-55] ab314650, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST
Low expression: liver (PMID: 16574740), kidney.
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.
All lanes: Western blot - Anti-ROR beta/RORB antibody [EPR27136-55] (Anti-ROR beta/RORB antibody [EPR27136-55] ab314650) at 1/1000 dilution
Lane 1: Human eyeball tissue lysate at 20 µg
Lane 2: Human kidney tissue lysate at 20 µg
Lane 3: Human liver tissue lysate at 20 µg
Secondary
All lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Observed band size: 53 kDa
Exposure time: 48s
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ROR beta/RORB antibody [EPR27136-55] - BSA and Azide free (ab314651)
This data was developed using Anti-ROR beta/RORB antibody [EPR27136-55] ab314650, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Rat kidney tissue labeling ROR beta/RORB with Anti-ROR beta/RORB antibody [EPR27136-55] ab314650 at 1/500 (1.022 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Negative control: No staining on rat kidney.The section was incubated with Anti-ROR beta/RORB antibody [EPR27136-55] ab314650 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ROR beta/RORB antibody [EPR27136-55] - BSA and Azide free (ab314651)
This data was developed using Anti-ROR beta/RORB antibody [EPR27136-55] ab314650, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Mouse kidney tissue labeling ROR beta/RORB with Anti-ROR beta/RORB antibody [EPR27136-55] ab314650 at 1/500 (1.022 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Negative control: No staining on mouse kidney.The section was incubated with Anti-ROR beta/RORB antibody [EPR27136-55] ab314650 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ROR beta/RORB antibody [EPR27136-55] - BSA and Azide free (ab314651)
This data was developed using Anti-ROR beta/RORB antibody [EPR27136-55] ab314650, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling ROR beta/RORB with Anti-ROR beta/RORB antibody [EPR27136-55] ab314650 at 1/100 (5.11 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Negative control: No staining on human kidney.The section was incubated with Anti-ROR beta/RORB antibody [EPR27136-55] ab314650 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ROR beta/RORB antibody [EPR27136-55] - BSA and Azide free (ab314651)
This data was developed using Anti-ROR beta/RORB antibody [EPR27136-55] ab314650, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Rat retina tissue labeling ROR beta/RORB with Anti-ROR beta/RORB antibody [EPR27136-55] ab314650 at 1/500 (1.022 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on rat retina.The section was incubated with Anti-ROR beta/RORB antibody [EPR27136-55] ab314650 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ROR beta/RORB antibody [EPR27136-55] - BSA and Azide free (ab314651)
This data was developed using Anti-ROR beta/RORB antibody [EPR27136-55] ab314650, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Rat cerebrum tissue labeling ROR beta/RORB with Anti-ROR beta/RORB antibody [EPR27136-55] ab314650 at 1/500 (1.022 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on rat cerebrum.The section was incubated with Anti-ROR beta/RORB antibody [EPR27136-55] ab314650 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ROR beta/RORB antibody [EPR27136-55] - BSA and Azide free (ab314651)
This data was developed using Anti-ROR beta/RORB antibody [EPR27136-55] ab314650, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Mouse retina tissue labeling ROR beta/RORB with Anti-ROR beta/RORB antibody [EPR27136-55] ab314650 at 1/500 (1.022 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on mouse retina.The section was incubated with Anti-ROR beta/RORB antibody [EPR27136-55] ab314650 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ROR beta/RORB antibody [EPR27136-55] - BSA and Azide free (ab314651)
This data was developed using Anti-ROR beta/RORB antibody [EPR27136-55] ab314650, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue labeling ROR beta/RORB with Anti-ROR beta/RORB antibody [EPR27136-55] ab314650 at 1/500 (1.022 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on mouse cerebrum (PMID: 34272258).The section was incubated with Anti-ROR beta/RORB antibody [EPR27136-55] ab314650 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ROR beta/RORB antibody [EPR27136-55] - BSA and Azide free (ab314651)
This data was developed using Anti-ROR beta/RORB antibody [EPR27136-55] ab314650, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Human retina tissue labeling ROR beta/RORB with Anti-ROR beta/RORB antibody [EPR27136-55] ab314650 at 1/100 (5.11 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on human retina.The section was incubated with Anti-ROR beta/RORB antibody [EPR27136-55] ab314650 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ROR beta/RORB antibody [EPR27136-55] - BSA and Azide free (ab314651)
This data was developed using Anti-ROR beta/RORB antibody [EPR27136-55] ab314650, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Human cerebrum tissue labeling ROR beta/RORB with Anti-ROR beta/RORB antibody [EPR27136-55] ab314650 at 1/100 (5.11 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on human cerebrum.The section was incubated with Anti-ROR beta/RORB antibody [EPR27136-55] ab314650 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins