AB314651

Anti-ROR beta/RORB antibody [EPR27136-55] - BSA and Azide free

BOND RX™ Validated
RabMAb
Recombinant
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(1 Publication)

Rabbit Recombinant Monoclonal ROR beta/RORB antibody. Carrier free. Suitable for IHC-P, WB and reacts with Human, Mouse, Rat samples. Cited in 1 publication.

View Alternative Names

Nr1f2, Rorb, Nuclear receptor ROR-beta, Nuclear receptor RZR-beta, Nuclear receptor subfamily 1 group F member 2, Retinoid-related orphan receptor-beta

12 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ROR beta/RORB antibody [EPR27136-55] - BSA and Azide free (AB314651)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ROR beta/RORB antibody [EPR27136-55] - BSA and Azide free (AB314651)

This data was developed using ab314650, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling ROR beta/RORB with ab314650 at 1/100 (5.11 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Negative control : No staining on human kidney.The section was incubated with ab314650 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ROR beta/RORB antibody [EPR27136-55] - BSA and Azide free (AB314651)

This data was developed using ab314650, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded Human cerebrum tissue labeling ROR beta/RORB with ab314650 at 1/100 (5.11 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on human cerebrum.The section was incubated with ab314650 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ROR beta/RORB antibody [EPR27136-55] - BSA and Azide free (AB314651)

This data was developed using ab314650, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded Human retina tissue labeling ROR beta/RORB with ab314650 at 1/100 (5.11 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on human retina.The section was incubated with ab314650 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ROR beta/RORB antibody [EPR27136-55] - BSA and Azide free (AB314651)

This data was developed using ab314650, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded Rat retina tissue labeling ROR beta/RORB with ab314650 at 1/500 (1.022 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on rat retina.The section was incubated with ab314650 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ROR beta/RORB antibody [EPR27136-55] - BSA and Azide free (AB314651)

This data was developed using ab314650, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue labeling ROR beta/RORB with ab314650 at 1/500 (1.022 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on mouse cerebrum (PMID : 34272258).The section was incubated with ab314650 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ROR beta/RORB antibody [EPR27136-55] - BSA and Azide free (AB314651)

This data was developed using ab314650, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded Rat cerebrum tissue labeling ROR beta/RORB with ab314650 at 1/500 (1.022 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on rat cerebrum.The section was incubated with ab314650 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ROR beta/RORB antibody [EPR27136-55] - BSA and Azide free (AB314651)

This data was developed using ab314650, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded Rat kidney tissue labeling ROR beta/RORB with ab314650 at 1/500 (1.022 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Negative control : No staining on rat kidney.The section was incubated with ab314650 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ROR beta/RORB antibody [EPR27136-55] - BSA and Azide free (AB314651)

This data was developed using ab314650, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded Mouse kidney tissue labeling ROR beta/RORB with ab314650 at 1/500 (1.022 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Negative control : No staining on mouse kidney.The section was incubated with ab314650 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ROR beta/RORB antibody [EPR27136-55] - BSA and Azide free (AB314651)

This data was developed using ab314650, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded Mouse retina tissue labeling ROR beta/RORB with ab314650 at 1/500 (1.022 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on mouse retina.The section was incubated with ab314650 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

Supplier Data

Western blot - Anti-ROR beta/RORB antibody [EPR27136-55] - BSA and Azide free (AB314651)

This data was developed using ab314650, the same antibody clone in a different buffer formulation. Blocking and diluting buffer and concentration : 5% NFDM/TBST The band beneath the target band (53 kDa) is likely to be degraded target fragments. Lysates were freshly made and used for Western blotting immediately to minimize protein degradation.

All lanes:

Western blot - Anti-ROR beta/RORB antibody [EPR27136-55] (<a href='/en-us/products/primary-antibodies/ror-beta-rorb-antibody-epr27136-55-ab314650'>ab314650</a>) at 1/1000 dilution

All lanes:

Y79 (human retinoblastoma retinoblastoma) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 40 kDa,53 kDa

false

Exposure time: 125s

Supplier Data

Western blot - Anti-ROR beta/RORB antibody [EPR27136-55] - BSA and Azide free (AB314651)

This data was developed using ab314650, the same antibody clone in a different buffer formulation. Blocking and diluting buffer and concentration : 5% NFDM/TBST Low expression : liver (PMID : 16574740), kidney. In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-ROR beta/RORB antibody [EPR27136-55] (<a href='/en-us/products/primary-antibodies/ror-beta-rorb-antibody-epr27136-55-ab314650'>ab314650</a>) at 1/1000 dilution

Lane 1:

Human eyeball tissue lysate at 20 µg

Lane 2:

Human kidney tissue lysate at 20 µg

Lane 3:

Human liver tissue lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Observed band size: 53 kDa

false

Exposure time: 48s

Supplier Data

Western blot - Anti-ROR beta/RORB antibody [EPR27136-55] - BSA and Azide free (AB314651)

This data was developed using ab314650, the same antibody clone in a different buffer formulation. Blocking and diluting buffer and concentration : 5% NFDM/TBST Low expression : liver (PMID : 16574740), kidney. The bands beneath the target band (53 kDa) are likely to be degraded target fragments. In Western blot, Anti-Vinculin antibody [EPR8185] (ab129002) staining at 1/10000 dilution.

All lanes:

Western blot - Anti-ROR beta/RORB antibody [EPR27136-55] (<a href='/en-us/products/primary-antibodies/ror-beta-rorb-antibody-epr27136-55-ab314650'>ab314650</a>) at 1/1000 dilution

Lane 1:

Mouse retina tissue lysate at 50 µg

Lane 2:

C57 P20 retina tissue lysate at 50 µg

Lane 3:

Mouse kidney tissue lysate at 50 µg

Lane 4:

Mouse liver tissue lysate at 50 µg

Lane 5:

Rat retina tissue lysate at 50 µg

Lane 6:

Rat kidney tissue lysate at 50 µg

Lane 7:

Rat liver tissue lysate at 50 µg

Secondary

All lanes:

Western blot - VeriBlot for IP Detection Reagent (HRP) (<a href='/en-us/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/1000 dilution

Observed band size: 20 kDa,30 kDa,53 kDa

false

Exposure time: 48s

Unconjugated

Anti-ROR beta/RORB antibody [EPR27136-55]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR27136-55

Isotype

IgG

Carrier free

Yes

Reacts with

Human, Mouse, Rat

Applications

WB, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Mouse": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Rat": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "" } } }

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Product details

ab314651 is the carrier-free version of ab314650.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Nuclear receptor that binds DNA as a monomer to ROR response elements (RORE) containing a single core motif half-site 5'-AGGTCA-3' preceded by a short A-T-rich sequence. Considered to have intrinsic transcriptional activity, have some natural ligands such as all-trans retinoic acid (ATRA) and other retinoids which act as inverse agonists repressing the transcriptional activity. Required for normal postnatal development of rod and cone photoreceptor cells. Modulates rod photoreceptors differentiation at least by inducing the transcription factor NRL-mediated pathway. In cone photoreceptor cells, regulates transcription of OPN1SW. Involved in the regulation of the period length and stability of the circadian rhythm. May control cytoarchitectural patterning of neocortical neurons during development. May act in a dose-dependent manner to regulate barrel formation upon innervation of layer IV neurons by thalamocortical axons. May play a role in the suppression of osteoblastic differentiation through the inhibition of RUNX2 transcriptional activity.. Isoform 1 is critical for hindlimb motor control and for the differentiation of amacrine and horizontal cells in the retina. Regulates the expression of PTF1A synergistically with FOXN4.

See full target information Rorb

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Cell adhesion & migration 18:38-53 PubMed39533963

2024

FeO nanoparticles containing gambogic acid inhibit metastasis in colorectal cancer via the RORB/EMILIN1 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaodong Fan,Chunyang Lv,Meiling Xue,Peng Meng,Xiaoping Qian

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-ROR beta/RORB antibody [EPR27136-55] - BSA and Azide free

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ROR beta/RORB antibody [EPR27136-55] - BSA and Azide free (AB314651)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ROR beta/RORB antibody [EPR27136-55] - BSA and Azide free (AB314651)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ROR beta/RORB antibody [EPR27136-55] - BSA and Azide free (AB314651)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ROR beta/RORB antibody [EPR27136-55] - BSA and Azide free (AB314651)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ROR beta/RORB antibody [EPR27136-55] - BSA and Azide free (AB314651)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ROR beta/RORB antibody [EPR27136-55] - BSA and Azide free (AB314651)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ROR beta/RORB antibody [EPR27136-55] - BSA and Azide free (AB314651)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ROR beta/RORB antibody [EPR27136-55] - BSA and Azide free (AB314651)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ROR beta/RORB antibody [EPR27136-55] - BSA and Azide free (AB314651)

Western blot - Anti-ROR beta/RORB antibody [EPR27136-55] - BSA and Azide free (AB314651)

Western blot - Anti-ROR beta/RORB antibody [EPR27136-55] - BSA and Azide free (AB314651)

Western blot - Anti-ROR beta/RORB antibody [EPR27136-55] - BSA and Azide free (AB314651)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Product protocols

Target data

Publications (1)

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Primary Antibodies

Primary Antibodies

Primary Antibodies

Product promise