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Mouse Recombinant Monoclonal ROR1 antibody. Suitable for WB and reacts with Transfected cell lysate - Human, Human samples.

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Images

Western blot - Anti-ROR1 antibody [6D4] (AB321998), expandable thumbnail
  • Western blot - Anti-ROR1 antibody [6D4] (AB321998), expandable thumbnail

Key facts

Isotype
IgG1
Host species
Mouse
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WB
Human
Tested
Transfected cell lysate - Human
Tested

Tested
Tested

Species
Transfected cell lysate - Human
Dilution info
1/1000
Notes

-

Species
Human
Dilution info
1/1000
Notes

-

Target data

Function

Has very low kinase activity in vitro and is unlikely to function as a tyrosine kinase in vivo (PubMed:25029443). Receptor for ligand WNT5A which activate downstream NFkB signaling pathway and may result in the inhibition of WNT3A-mediated signaling (PubMed:25029443, PubMed:27162350). In inner ear, crucial for spiral ganglion neurons to innervate auditory hair cells (PubMed:27162350). Via IGFBP5 ligand, forms a complex with ERBB2 to enhance CREB oncogenic signaling (PubMed:36949068).

Alternative names

NTRKR1, ROR1, Inactive tyrosine-protein kinase transmembrane receptor ROR1

Recommended products

Mouse Recombinant Monoclonal ROR1 antibody. Suitable for WB and reacts with Transfected cell lysate - Human, Human samples.

Key facts

Isotype
IgG1
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
6D4
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

2 product images

  • Western blot - Anti-ROR1 antibody [6D4] (ab321998), expandable thumbnail

    Western blot - Anti-ROR1 antibody [6D4] (ab321998)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    In Western blot, Anti-Histone H3 antibody [EPR16987] - Nuclear Marker and ChIP Grade (Anti-Histone H3 antibody [EPR16987] - Nuclear Marker and ChIP Grade ab176842) staining at 1/100000 dilution.

    In Western blot, Anti-6X His tag® antibody [EPR20547] - ChIP Grade (Anti-6X His tag® antibody [EPR20547] - ChIP Grade ab213204) staining at 1/5000 dilution.

    All lanes: Western blot - Anti-ROR1 antibody [6D4] (ab321998) at 1/1000 dilution

    Lane 1: 293T (human embryonic kidney epithelial cell) transfected with an empty vector (vector control) containing a myc-His-tag® whole cell lysate at 20 µg

    Lane 2: 293T transfected with a ROR2 expression vector containing a myc-His-tag® whole cell lysate at 20 µg

    Lane 3: 293T transfected with a ROR1 expression vector containing a myc-His-tag® whole cell lysate at 20 µg

    Secondary

    All lanes: Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution

    Observed band size: 130 Da, 15 kDa, 46 kDa

    Exposure time: 1s

  • Western blot - Anti-ROR1 antibody [6D4] (ab321998), expandable thumbnail

    Western blot - Anti-ROR1 antibody [6D4] (ab321998)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Negative control: K-562 (PMID: 27852699), MCF7 (PMID: 22403610)

    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

    All lanes: Western blot - Anti-ROR1 antibody [6D4] (ab321998) at 1/1000 dilution

    Lane 1: PANC-1 (human pancreatic epithelioid carcinoma epithelial cell) whole cell lysate at 20 µg

    Lane 2: NCI-H1975 (human adenocarcinoma lung epithelial cell) whole cell lysate at 20 µg

    Lane 3: K-562 (human chronic myelogenous leukemia lymphoblast) whole cell lysate at 20 µg

    Lane 4: MCF7 (human breast adenocarcinoma epithelial cell) whole cell lysate at 20 µg

    Secondary

    All lanes: Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution

    Observed band size: 130 kDa, 36 kDa

    Exposure time: 180s

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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