Rabbit Recombinant Monoclonal ROR1 antibody. Suitable for Flow Cyt and reacts with Human samples.
Images
![Flow Cytometry (Intracellular) - Anti-ROR1 antibody [EPR24451-141] (AB280197), expandable thumbnail](https://content.abcam.com/products/images/ror1-antibody-epr24451-141-ab280197--flow-cytometry-intracellular-img15546.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-ROR1 antibody [EPR24451-141] (AB280197)")
![Flow Cytometry (Intracellular) - Anti-ROR1 antibody [EPR24451-141] (AB280197), expandable thumbnail](https://content.abcam.com/products/images/ror1-antibody-epr24451-141-ab280197--flow-cytometry-intracellular-img13536.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-ROR1 antibody [EPR24451-141] (AB280197)")
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 59.94% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
Liquid
- Clonality
Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| ICC/IF | IP | WB | IHC-P | Flow Cyt | |
|---|---|---|---|---|---|
| Human | Not recommended | Not recommended | Not recommended | Not recommended | Tested |
| Mouse | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
| Rat | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Human, Rat | Dilution info - | Notes - |
WB
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
Flow Cyt
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500 | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Target data
Function
Has very low kinase activity in vitro and is unlikely to function as a tyrosine kinase in vivo (PubMed:25029443). Receptor for ligand WNT5A which activate downstream NFkB signaling pathway and may result in the inhibition of WNT3A-mediated signaling (PubMed:25029443, PubMed:27162350). In inner ear, crucial for spiral ganglion neurons to innervate auditory hair cells (PubMed:27162350).
Alternative names
Inactive tyrosine-protein kinase transmembrane receptor ROR1, ROR1, NTRKR1
Recommended products
Rabbit Recombinant Monoclonal ROR1 antibody. Suitable for Flow Cyt and reacts with Human samples.
Alternative names
Inactive tyrosine-protein kinase transmembrane receptor ROR1, ROR1, NTRKR1
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 59.94% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
Liquid
- Clonality
Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
EPR24451-141
- Purification technique
Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage duration
1-2 weeks
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
![Flow Cytometry (Intracellular) - Anti-ROR1 antibody [EPR24451-141] (ab280197), expandable thumbnail](https://content.abcam.com/products/images/ror1-antibody-epr24451-141-ab280197--flow-cytometry-intracellular-img15546.jpg "Flow Cytometry (Intracellular) - Anti-ROR1 antibody [EPR24451-141] (ab280197)")
Flow Cytometry (Intracellular) - Anti-ROR1 antibody [EPR24451-141] (ab280197)
Flow cytometric analysis of MCF7 (Human breast adenocarcinoma epithelial cell, Left) /PANC-1 (Human pancreatic epithelioid carcinoma epithelial cell, Right) cells labelling ROR1 with ab280197 at 1/500 dilution compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat anti rabbit IgG (Alexa Fluor® 647, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 647) ab150079) at 1/2000 dilution was used as the secondary antibody. Negative control: MCF7 (PMID:25835638, 26862065) Gated on viable cells.
![Flow Cytometry (Intracellular) - Anti-ROR1 antibody [EPR24451-141] (ab280197), expandable thumbnail](https://content.abcam.com/products/images/ror1-antibody-epr24451-141-ab280197--flow-cytometry-intracellular-img13536.jpg "Flow Cytometry (Intracellular) - Anti-ROR1 antibody [EPR24451-141] (ab280197)")
Flow Cytometry (Intracellular) - Anti-ROR1 antibody [EPR24451-141] (ab280197)
Flow cytometric analysis of K-562 (Human chronic myelogenous leukemia lymphoblast, Left) /MDA-MB-231 (human breast adenocarcinoma epithelial cell, Right) cells labelling ROR1 with ab280197 at 1/500 dilution compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/2000 dilution was used as the secondary antibody. Negative control: K-562 (PMID:25835638, 27852699) Gated on viable cells.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
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