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AB280200

Anti-ROR1 antibody [EPR24451-141] - BSA and Azide free

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Rabbit Recombinant Monoclonal ROR1 antibody. Carrier free. Suitable for Flow Cyt and reacts with Human samples.

View Alternative Names

NTRKR1, ROR1, Inactive tyrosine-protein kinase transmembrane receptor ROR1

2 Images
Flow Cytometry (Intracellular) - Anti-ROR1 antibody [EPR24451-141] - BSA and Azide free (AB280200)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-ROR1 antibody [EPR24451-141] - BSA and Azide free (AB280200)

This data was developed using ab280197, the same antibody clone in a different buffer formulation.

Intracellular flow cytometric analysis of MCF7 (Human breast adenocarcinoma epithelial cell, Left) /PANC-1 (Human pancreatic epithelioid carcinoma epithelial cell, Right) cells labelling ROR1 with ab280197 at 1/500 dilution compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat anti rabbit IgG (Alexa Fluor® 647, ab150079) at 1/2000 dilution was used as the secondary antibody. Negative control : MCF7 (PMID : 25835638, 26862065) Gated on viable cells.

Flow Cytometry (Intracellular) - Anti-ROR1 antibody [EPR24451-141] - BSA and Azide free (AB280200)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-ROR1 antibody [EPR24451-141] - BSA and Azide free (AB280200)

This data was developed using ab280197, the same antibody clone in a different buffer formulation.

Intracellular flow cytometric analysis of K-562 (Human chronic myelogenous leukemia lymphoblast, Left) /MDA-MB-231 (human breast adenocarcinoma epithelial cell, Right) cells labelling ROR1 with ab280197 at 1/500 dilution compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody. Negative control : K-562 (PMID : 25835638, 27852699) Gated on viable cells.

  • Unconjugated

    Anti-ROR1 antibody [EPR24451-141]

  • 519 Alexa Fluor® 488

    Alexa Fluor® 488 Anti-ROR1 antibody [EPR24451-141]

  • 665 Alexa Fluor® 647

    Alexa Fluor® 647 Anti-ROR1 antibody [EPR24451-141]

  • 578 PE

    PE Anti-ROR1 antibody [EPR24451-141]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR24451-141

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

Flow Cyt

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Complementary Products

Assay Kits

AB267606

Human ROR1 ELISA Kit

Sandwich ELISA - Human ROR1 ELISA Kit (AB267606)

  • 0
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Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

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Primary Antibodies

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ErbB3 / HER3 antibody [SP71] (AB93739)

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Primary Antibodies

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Anti-TROP2 antibody [EPR20043]

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Western blot - Anti-TROP2 antibody [EPR20043] (AB214488)

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Primary Antibodies

AB215729

Anti-GSDMB antibody [EPR20841]

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Flow Cytometry (Intracellular) - Anti-GSDMB antibody [EPR20841] (AB215729)

  • 0
  • 0
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Reactivity data

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Product details

ab280200 is the carrier-free version of ab280197.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Has very low kinase activity in vitro and is unlikely to function as a tyrosine kinase in vivo (PubMed : 25029443). Receptor for ligand WNT5A which activate downstream NFkB signaling pathway and may result in the inhibition of WNT3A-mediated signaling (PubMed : 25029443, PubMed : 27162350). In inner ear, crucial for spiral ganglion neurons to innervate auditory hair cells (PubMed : 27162350). Via IGFBP5 ligand, forms a complex with ERBB2 to enhance CREB oncogenic signaling (PubMed : 36949068).
See full target information ROR1
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com