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AB245456

Anti-ROR2 antibody

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(3 Publications)

Rabbit Polyclonal ROR2 antibody. Suitable for IP, WB and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human ROR2 aa 850 to C-terminus.

View Alternative Names

NTRKR2, ROR2, Tyrosine-protein kinase transmembrane receptor ROR2

2 Images
Immunoprecipitation - Anti-ROR2 antibody (AB245456)
  • IP

Supplier Data

Immunoprecipitation - Anti-ROR2 antibody (AB245456)

ROR2 was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab245456 at 6 μg/mg lysate. Western blot was performed from the immunoprecipitate using ab245456 at 1 μg/ml.

Lane 1 : ab245456 IP in HEK-293T whole cell lysate.
Lane 2 : Control IgG IP in HEK-293T whole cell lysate.

Detection : Chemiluminescence with exposure time of 10 seconds.

All lanes:

Immunoprecipitation - Anti-ROR2 antibody (ab245456)

Predicted band size: 105 kDa

false

Western blot - Anti-ROR2 antibody (AB245456)
  • WB

Supplier Data

Western blot - Anti-ROR2 antibody (AB245456)

All lanes:

Western blot - Anti-ROR2 antibody (ab245456) at 0.1 µg/mL

Lane 1:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg

Lane 2:

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg

Lane 3:

Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate at 50 µg

Predicted band size: 105 kDa

true

Exposure time: 10s

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, IP

applications

Immunogen

Synthetic Peptide within Human ROR2 aa 850 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q01974

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-10 µg/mg of lysate", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "<p></p>" } } }

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
ab245456 was affinity purified using an epitope specific to ROR2 immobilized on solid support.
Storage buffer
pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: Tris citrate/phosphate
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Target data

Tyrosine-protein kinase receptor which may be involved in the early formation of the chondrocytes. It seems to be required for cartilage and growth plate development (By similarity). Phosphorylates YWHAB, leading to induction of osteogenesis and bone formation (PubMed : 17717073). In contrast, has also been shown to have very little tyrosine kinase activity in vitro. May act as a receptor for wnt ligand WNT5A which may result in the inhibition of WNT3A-mediated signaling (PubMed : 25029443).
See full target information ROR2

Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Journal of bone oncology 33:100412 PubMed35198364

2022

MiR-451a promotes cell growth, migration and EMT in osteosarcoma by regulating YTHDC1-mediated m6A methylation to activate the AKT/mTOR signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Dong Cao,Shanshan Ge,Mengchun Li

Histology and histopathology 37:513-526 PubMed35037694

2022

Circ_0044520 regulates the progression of laryngeal squamous cell carcinoma via the miR-338-3p/ROR2 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Huijun Yang,Gang Yu,Yan Wang,Xing Guo

Cardiovascular therapeutics 2020:6758934 PubMed32565910

2020

Long Noncoding RNA TUG1 Promotes the Function in ox-LDL-Treated HA-VSMCs via miR-141-3p/ROR2 Axis.

Applications

Unspecified application

Species

Unspecified reactive species

Yu Tang,Jing Hu,Zhiying Zhong,Yanfeng Liu,Yunxia Wang
View all publications

Product promise

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