Anti-ROR2 antibody [EPR19980]

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ROR2 antibody [EPR19980] (AB218105)

Immunohistochemical analysis of paraffin-embedded human bladder cancer tissue labeling ROR2 with ab218105 at 1/500 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

Membranous staining on human bladder cancer is observed.

Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ROR2 antibody [EPR19980] (AB218105)

Immunohistochemical analysis of paraffin-embedded human breast cancer tissue labeling ROR2 with ab218105 at 1/500 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

Membranous staining on human breast cancer is observed.

Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ROR2 antibody [EPR19980] (AB218105)

Immunohistochemical analysis of paraffin-embedded human breast tissue labeling ROR2 with ab218105 at 1/500 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

Positive membranous staining on human breast stroma cells; negative staining on epithelium cells.

Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ROR2 antibody [EPR19980] (AB218105)

Immunohistochemical analysis of paraffin-embedded human pancreas tissue labeling ROR2 with ab218105 at 1/500 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

Positive membranous staining on human pancreas blood vessels; negative staining on epithelium cell.

Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ROR2 antibody [EPR19980] (AB218105)

Immunohistochemical analysis of paraffin-embedded human pancreatic cancer tissue labeling ROR2 with ab218105 at 1/500 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

Membranous staining on human pancreatic cancer is observed.

Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ROR2 antibody [EPR19980] (AB218105)

Immunohistochemical analysis of paraffin-embedded human gastrointestinal stromal tumors labeling ROR2 with ab218105 at 1/500 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

Cytoplasmic membrane staining on human gastrointestinal stromal tumors is observed.

Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

• WB

Lab

Western blot - Anti-ROR2 antibody [EPR19980] (AB218105)

Western blot : Anti-ROR2 antibody [EPR19980] (ab218105) staining at 1/1000 dilution, shown in green; Mouse anti-Alpha Tubulin [DM1A] (ab7291) loading control staining at 1/20000 dilution, shown in magenta. In Western blot, ab218105 was shown to bind specifically to ROR2. A band was observed at 105-130 kDa in wild-type HAP1 cell lysates with no signal observed at this size in ROR2 knockout cell line. To generate this image, wild-type and ROR2 knockout HAP1 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween^® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution.

All lanes:

Western blot - Anti-ROR2 antibody [EPR19980] (ab218105) at 1/1000 dilution

Lane 1:

Wild-type HAP1 cell lysate at 20 µg

Lane 2:

ROR2 knockout HAP1 cell lysate at 20 µg

Lane 2:

Western blot - Human ROR2 knockout Raji cell line (<a href='/en-us/products/cell-lines/human-ror2-knockout-raji-cell-line-ab290368'>ab290368</a>)

Lane 3:

HeLa cell lysate at 20 µg

Lane 4:

HepG2 cell lysate at 20 µg

Secondary

All lanes:

Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution

Observed band size: 105-130 kDa

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