Anti-ROS1 antibody [EPMGHR2] - BSA and Azide free

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ROS1 antibody [EPMGHR2] - BSA and Azide free (AB238986)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lung carcinoma tissue sections labeling ROS1 with Purified ab189925 at 1 : 300 dilution (5.23 μg/ml). Heat mediated antigen retrieval was performed using EDTA Buffer, pH9.0. Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) secondary antibody was used at 1 : 0 dilution. PBS instead of the primary antibody was used as the negative control.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab189925).

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ROS1 antibody [EPMGHR2] - BSA and Azide free (AB238986)

Immunohistochemical analysis of paraffin-embedded Human lung carcinoma tissue labeling ROS1 with unpurified ab189925 at 1/250 dilution followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin. (Inset : negative control).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab189925).

Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

• WB

Lab

Western blot - Anti-ROS1 antibody [EPMGHR2] - BSA and Azide free (AB238986)

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab189925). Blocking buffer and concentration : 5% NFDM/TBST Diluting buffer and concentration : 5% NFDM /TBST We advise using U-118MG (FIG-ROS1 fusion) or HCC78 (SLC34A2-ROS1 fusion) as a positive cell line by WB. We have been unable to identify a positive control cell line for endogenous full-length ROS1. The identity of the lower MW band at approximately 60kDa is unknown. This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab189925).

All lanes:

Western blot - Anti-ROS1 antibody [EPMGHR2] (<a href='/en-us/products/primary-antibodies/ros1-antibody-epmghr2-ab189925'>ab189925</a>) at 1/1000 dilution

Lane 1:

U-118 MG (Human brain glioblastoma) whole cell lysate at 20 µg

Lane 2:

Saos-2 (Human osteosarcoma epithelial) whole cell lysate at 20 µg

Lane 3:

SW1353 (Human chondrosarcoma fibroblast) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 264 kDa

Observed band size: 100 kDa

false

Exposure time: 180s