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AB181695

Anti-Rotavirus antibody [A2]

5

(1 Review)

|

(9 Publications)

Mouse Monoclonal Rotavirus antibody. Suitable for WB, ICC/IF and reacts with Rotavirus samples. Cited in 9 publications.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

A2

Isotype

IgG2b

Light chain type

kappa

Carrier free

No

Reacts with

Rotavirus

Applications

WB, ICC/IF

applications

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Rotavirus": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "1/200 - 1/1000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "1/100 - 1/500", "ICCIF-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
Constituents: 2% Sucrose, 1.21% Tris, 0.75% Glycine
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Rotavirus also called RVA is a non-enveloped virus that primarily infects the gastrointestinal tract. It has a double-stranded RNA genome encoding six structural proteins and six non-structural proteins. The virus particles have a triple-layered capsid which is 70-75 nanometers in diameter. Rotavirus is expressed in the small intestine of humans and animals where it attaches to and enters mature enterocytes. The virus disrupts these cells’ function and leads to fluid loss and diarrhea.
Biological function summary

Rotavirus significantly impacts intestinal absorption and secretion balance. The virus produces non-structural protein 4 (NSP4) which functions as an enterotoxin. NSP4 alters intracellular calcium homeostasis and induces secretion resulting in diarrhea. Rotavirus is not part of a complex; its independent proteins perform roles that contribute to its pathogenicity and replication.

Pathways

Rotavirus infection affects host signaling pathways like NF-κB and interferon (IFN) responses. Disruption of these pathways helps the virus to circumvent immune elimination and propagate in the host. NSP4 interacts with host extracellular signal-regulated kinases (ERK) influencing cellular processes that give the virus a replication advantage. These pathways signal cellular responses that affect inflammation and cell apoptosis.

Rotavirus is mainly implicated in acute gastroenteritis especially in children under five. This condition can lead to severe dehydration and in extreme cases can be life-threatening without adequate treatment. There is a relation between Rotavirus and the protein β-defensin which the virus counteracts to evade innate immune defenses. Vaccination has proven effective in reducing disease incidence and associated healthcare burdens worldwide.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

See full target information Rotavirus
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Publications (9)

Recent publications for all applications. Explore the full list and refine your search

Pharmaceuticals (Basel, Switzerland) 16: PubMed37765086

2023

Metformin Hydrochloride Significantly Inhibits Rotavirus Infection in Caco2 Cell Line, Intestinal Organoids, and Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Rui Zhang,Cui Feng,Dandan Luo,Ruibo Zhao,Perumal Ramesh Kannan,Yuebang Yin,Muhammad Zubair Iqbal,Yeting Hu,Xiangdong Kong

Journal of virology 96:e0048822 PubMed35758692

2022

Using Species a Rotavirus Reverse Genetics to Engineer Chimeric Viruses Expressing SARS-CoV-2 Spike Epitopes.

Applications

Unspecified application

Species

Unspecified reactive species

Ola Diebold,Victoria Gonzalez,Luca Venditti,Colin Sharp,Rosemary A Blake,Wenfang S Tan,Joanne Stevens,Sarah Caddy,Paul Digard,Alexander Borodavka,Eleanor Gaunt

Scientific reports 12:6268 PubMed35428750

2022

Protective effects of the postbiotic deriving from cow's milk fermentation with L. paracasei CBA L74 against Rotavirus infection in human enterocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Cristina Bruno,Lorella Paparo,Laura Pisapia,Alessia Romano,Maddalena Cortese,Erika Punzo,Roberto Berni Canani

Molecular microbiology 117:818-836 PubMed34954851

2022

Rotavirus activates MLKL-mediated host cellular necroptosis concomitantly with apoptosis to facilitate dissemination of viral progeny.

Applications

Unspecified application

Species

Unspecified reactive species

Urbi Mukhopadhyay,Upayan Patra,Pritam Chandra,Priyanka Saha,Animesh Gope,Moumita Dutta,Mamta Chawla-Sarkar

Methods in molecular biology (Clifton, N.J.) 2132:585-595 PubMed32306358

2020

Screening for Components/Compounds with Anti-Rotavirus Activity: Detection of Interaction Between Viral Spike Proteins and Glycans.

Applications

Unspecified application

Species

Unspecified reactive species

Keita Yamada,Junko Nio-Kobayashi,Mizuho Inagaki

Journal of nanobiotechnology 17:13 PubMed30670042

2019

A milk-based self-assemble rotavirus VP6-ferritin nanoparticle vaccine elicited protection against the viral infection.

Applications

Unspecified application

Species

Unspecified reactive species

Zhipeng Li,Kuiqing Cui,Hong Wang,Fuhang Liu,Kongwei Huang,Zhaojun Duan,Fengchao Wang,Deshun Shi,Qingyou Liu

Virus research 253:28-37 PubMed29859235

2018

TNF-α exerts potent anti-rotavirus effects via the activation of classical NF-κB pathway.

Applications

ICC/IF

Species

Unspecified reactive species

Mohamad S Hakim,Shihao Ding,Sunrui Chen,Yuebang Yin,Junhong Su,C Janneke van der Woude,Gwenny M Fuhler,Maikel P Peppelenbosch,Qiuwei Pan,Wenshi Wang

Scientific reports 8:8341 PubMed29844362

2018

Basal interferon signaling and therapeutic use of interferons in controlling rotavirus infection in human intestinal cells and organoids.

Applications

Unspecified application

Species

Unspecified reactive species

Mohamad S Hakim,Sunrui Chen,Shihao Ding,Yuebang Yin,Aqsa Ikram,Xiao-Xia Ma,Wenshi Wang,Maikel P Peppelenbosch,Qiuwei Pan

Scientific reports 6:25120 PubMed27112594

2016

MYH9 is an Essential Factor for Porcine Reproductive and Respiratory Syndrome Virus Infection.

Applications

Unspecified application

Species

Unspecified reactive species

Jiming Gao,Shuqi Xiao,Yihong Xiao,Xiangpeng Wang,Chong Zhang,Qin Zhao,Yuchen Nan,Baicheng Huang,Hongliang Liu,Ningning Liu,Junhua Lv,Taofeng Du,Yani Sun,Yang Mu,Gang Wang,Shahid Faraz Syed,Gaiping Zhang,Julian A Hiscox,Ian Goodfellow,En-Min Zhou
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

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