Rabbit Recombinant Monoclonal RP105 antibody. Suitable for WB, ICC/IF, IHC-P and reacts with Human samples. Cited in 2 publications.
Images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RP105 antibody [EPR14720] (AB184956), expandable thumbnail](https://content.abcam.com/products/images/rp105-antibody-epr14720-ab184956--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img128454.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RP105 antibody [EPR14720] (AB184956)")
![Western blot - Anti-RP105 antibody [EPR14720] (AB184956), expandable thumbnail](https://content.abcam.com/products/images/rp105-antibody-epr14720-ab184956--western-blot-img57665.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-RP105 antibody [EPR14720] (AB184956)")
![Western blot - Anti-RP105 antibody [EPR14720] (AB184956), expandable thumbnail](https://content.abcam.com/products/images/rp105-antibody-epr14720-ab184956--western-blot-img81838.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-RP105 antibody [EPR14720] (AB184956)")
![Immunocytochemistry/ Immunofluorescence - Anti-RP105 antibody [EPR14720] (AB184956), expandable thumbnail](https://content.abcam.com/products/images/rp105-antibody-epr14720-ab184956--immunocytochemistry-immunofluorescence-img93361.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-RP105 antibody [EPR14720] (AB184956)")
Publications
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- JCI insight 7:2022Deficiency of inhibitory TLR4 homolog RP105 exacerbates fibrosis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWenxia Wang,Swarna Bale,Bharath Yalavarthi,Priyanka Verma,Pei-Suen Tsou,Ken M Calderone,Dibyendu Bhattacharyya,Gary J Fisher,John Varga,Swati BhattacharyyaPubMed 36136452
- Journal of cellular biochemistry 120:16888-168992019Inhibition of myeloid differentiation 1 specifically in colon with antisense oligonucleotide exacerbates dextran sodium sulfate-induced colitis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXiaoxing Chen et. al.PubMed 31104313
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | Flow Cyt | WB | ICC/IF | IHC-P | |
|---|---|---|---|---|---|
| Human | Not recommended | Not recommended | Tested | Tested | Tested |
| Mouse | Not recommended | Not recommended | Predicted | Predicted | Predicted |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse | Dilution info - | Notes - |
Flow Cyt
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Mouse | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/1000 - 1/2000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
Associated Products
Select an associated product type
1 product for Alternative Product
Target data
Function
May cooperate with MD-1 and TLR4 to mediate the innate immune response to bacterial lipopolysaccharide (LPS) in B-cells. Leads to NF-kappa-B activation. Also involved in the life/death decision of B-cells (By similarity).
Alternative names
CD180, LY64, RP105, CD180 antigen, Lymphocyte antigen 64, Radioprotective 105 kDa protein
Recommended products
Rabbit Recombinant Monoclonal RP105 antibody. Suitable for WB, ICC/IF, IHC-P and reacts with Human samples. Cited in 2 publications.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EPR14720
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
4 product images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RP105 antibody [EPR14720] (ab184956), expandable thumbnail](https://content.abcam.com/products/images/rp105-antibody-epr14720-ab184956--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img128454.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RP105 antibody [EPR14720] (ab184956)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RP105 antibody [EPR14720] (ab184956)
Immunohistochemical analysis of paraffin-embedded human spleen tissue labeling RP105 with ab184956 at 1/500 dilution and HRP Polymer to Rabbit IgG. Counterstained with Hematoxylin.
Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
![Western blot - Anti-RP105 antibody [EPR14720] (ab184956), expandable thumbnail](https://content.abcam.com/products/images/rp105-antibody-epr14720-ab184956--western-blot-img57665.jpg "Western blot - Anti-RP105 antibody [EPR14720] (ab184956)")
Western blot - Anti-RP105 antibody [EPR14720] (ab184956)
All lanes: Western blot - Anti-RP105 antibody [EPR14720] (ab184956) at 1/2000 dilution
Lane 1: Human lymph node lysate at 20 µg
Lane 2: Ramos cell lysate at 20 µg
SecondaryAll lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 74 kDa
Observed band size: 90 kDa
![Western blot - Anti-RP105 antibody [EPR14720] (ab184956), expandable thumbnail](https://content.abcam.com/products/images/rp105-antibody-epr14720-ab184956--western-blot-img81838.jpg "Western blot - Anti-RP105 antibody [EPR14720] (ab184956)")
Western blot - Anti-RP105 antibody [EPR14720] (ab184956)
All lanes: Western blot - Anti-RP105 antibody [EPR14720] (ab184956) at 1/2000 dilution
All lanes: Daudi cell lysate at 10 µg
SecondaryAll lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 74 kDa
Observed band size: 90 kDa
![Immunocytochemistry/ Immunofluorescence - Anti-RP105 antibody [EPR14720] (ab184956), expandable thumbnail](https://content.abcam.com/products/images/rp105-antibody-epr14720-ab184956--immunocytochemistry-immunofluorescence-img93361.jpg "Immunocytochemistry/ Immunofluorescence - Anti-RP105 antibody [EPR14720] (ab184956)")
Immunocytochemistry/ Immunofluorescence - Anti-RP105 antibody [EPR14720] (ab184956)
Immunocytochemical analysis of Ramos cells labeling RP105 with ab184956 at 1/100 dilution and Goat anti rabbit IgG (Alexa Fluor®488) at 1/200 dilution. Counterstained with DAPI. Cells fixation: -20℃ Acetone.
Downloads
Product protocols
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