Anti-RP105 antibody [EPR14720] - BSA and Azide free

• ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-RP105 antibody [EPR14720] - BSA and Azide free (AB250792)

Immunofluorescent analysis of 80% Methanol, 0.1% Triton X-100 permeabilized WEHI-231 (mouse B cell lymphoma B lymphocyte) cells with ab184956 at 1/50 dilution concentration followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 (2 μg/ml dilution (Green). Image showing cytoplasmic staining in WEHI-231 cell line (shown in green). ab7291 Anti-alpha Tubulin antibody [DM1A] was used to counterstain tubulin at 1/1000 dilution (Magenta) and ab150120 Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) was used as secondary counterstain at 1/1000 dilution. The nuclear counterstain was DAPI (Blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). Negative control : NIH/3T3.

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-RP105 antibody [EPR14720] - BSA and Azide free (AB250792)

This data was developed using ab184956, the same antibody clone in a different buffer formulation.

Immunocytochemical analysis of Ramos cells labeling RP105 with ab184956 at 1/100 dilution and Goat anti rabbit IgG (Alexa Fluor®488) at 1/200 dilution. Counterstained with DAPI. Cells fixation : -20°C Acetone.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RP105 antibody [EPR14720] - BSA and Azide free (AB250792)

This data was developed using ab184956, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded human spleen tissue labeling RP105 with ab184956 at 1/500 dilution and HRP Polymer to Rabbit IgG. Counterstained with Hematoxylin.

Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RP105 antibody [EPR14720] - BSA and Azide free (AB250792)

This data was developed using ab184956, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of formalin fixed paraffin embedded mouse lung labelling RP105 with ab184956 at a concentration of 1/2000 dilution. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins. The primary antibody Anti-RP105 antibody [EPR14720] was incubated for 30 mins at room temperature. Sections were counterstained with Hematoxylin II. Positive staining on mouse lung.

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RP105 antibody [EPR14720] - BSA and Azide free (AB250792)

This data was developed using ab184956, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of formalin fixed paraffin embedded mouse skeletal muscle labelling RP105 with ab184956 at a concentration of 1/2000 dilution. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins. The primary antibody Anti-RP105 antibody [EPR14720] was incubated for 30 mins at room temperature. Sections were counterstained with Hematoxylin II. Low expression tissue : nearly no staining on mouse skeletal muscle.

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RP105 antibody [EPR14720] - BSA and Azide free (AB250792)

This data was developed using ab184956, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of formalin fixed paraffin embedded mouse spleen labelling RP105 with ab184956 at a concentration of 1/2000 dilution. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins. The primary antibody Anti-RP105 antibody [EPR14720] was incubated for 30 mins at room temperature. Sections were counterstained with Hematoxylin II. Positive staining on mouse spleen.

• WB

Supplier Data

Western blot - Anti-RP105 antibody [EPR14720] - BSA and Azide free (AB250792)

This data was developed using ab184956, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-RP105 antibody [EPR14720] (<a href='/en-us/products/primary-antibodies/rp105-antibody-epr14720-ab184956'>ab184956</a>) at 1/2000 dilution

All lanes:

Daudi cell lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 74 kDa

Observed band size: 90 kDa

false

• WB

Supplier Data

Western blot - Anti-RP105 antibody [EPR14720] - BSA and Azide free (AB250792)

This data was developed using ab184956, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-RP105 antibody [EPR14720] (<a href='/en-us/products/primary-antibodies/rp105-antibody-epr14720-ab184956'>ab184956</a>) at 1/2000 dilution

Lane 1:

Human lymph node lysate at 20 µg

Lane 2:

Ramos cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 74 kDa

Observed band size: 90 kDa

false

• WB

Lab

Western blot - Anti-RP105 antibody [EPR14720] - BSA and Azide free (AB250792)

This data was developed using ab184956, the same antibody clone in a different buffer formulation.

In Western blot, anti-GAPDH antibody (ab181602) loading control staining at 1/200000 dilution.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Negative control : NIH/3T3 Low expression : brain and skeletal muscle

All lanes:

Western blot - Anti-RP105 antibody [EPR14720] (<a href='/en-us/products/primary-antibodies/rp105-antibody-epr14720-ab184956'>ab184956</a>) at 1/1000 dilution

Lane 1:

WEHI-231 (mouse B cell lymphoma B lymphocyte ) whole cell lysate at 20 µg

Lane 2:

RAW 264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysate at 20 µg

Lane 3:

NIH/3T3 (mouse embryonic fibroblast) whole cell lysate at 20 µg

Lane 4:

Mouse spleen tissue lysate at 20 µg

Lane 5:

Mouse bone marrow tissue lysate at 20 µg

Lane 6:

Mouse brain tissue lysate at 20 µg

Lane 7:

Mouse skeletal muscle tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 90 kDa,36 kDa

false

Exposure time: 90s