Anti-RPA14/RPA3 antibody

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPA14/RPA3 antibody (AB97436)

Immunohistochemical analysis of RPA14/RPA3 in paraffin embedded lung CA patient tumor, using ab97436 at a 1/100 dilution.

• WB

Unknown

Western blot - Anti-RPA14/RPA3 antibody (AB97436)

All lanes:

Western blot - Anti-RPA14/RPA3 antibody (ab97436) at 1/1000 dilution

Lane 1:

HeLa whole cell lysate at 30 µg

Lane 2:

HepG2 whole cell lysate at 30 µg

Lane 3:

MOLT4 whole cell lysate at 30 µg

Predicted band size: 14 kDa

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• WB

CiteAb

Western blot - Anti-RPA14/RPA3 antibody (AB97436)

RPA14/RPA3 western blot using anti-RPA14/RPA3 antibody ab97436. Publication image and figure legend from Pedersen, H., Anne Adanma Obara, E., et al., 2020, Int J Mol Sci, PubMed 32111042.

ab97436 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab97436 please see the product overview.

RPA expression is crucial for the maintenance of glioblastoma cancer stem-like cells. (A) Immunoblot analysis of RPA70, RPA32 and RPA14 in a panel of GBM primary cell lines (4121, G01, G06, G40, G07, G16 and G20) and two normal human astrocyte cell lines (NHA33, NHA26). β-Actin was used as a loading control. (B) Immunoblot analysis of RPA70, RPA32 and RPA14 expression in matched pairs of glioblastoma cancer stem-like cells (GSCs) and differentiated GBM cells (DGCs) isolated from 4121, G01, G06 and G40 primary GBM cell lines. α-Tubulin or Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) was used as a loading control. (C) Immunoblot analysis validating knockdown efficiencies of lentiviral shRNAs targeting RPA70, RPA32 and RPA14 in G01-GSCs. α-Tubulin was used as a loading control. (D) CellTiter-Glo luminescent viability assay (Promega) of G01-GSCs transduced with lentiviral shRNA targeting RPA70, RPA32 or RPA14 assessed 72 h after virus wash. Data are presented as mean ± SD. Statistical significance was tested using a two-way ANOVA with post-hoc Sidak's multiple comparisons test, where *** p < 0.001. N = 3. (E) CellTiter-Glo luminescent viability assay (Promega) of G01-GSCs transduced with lentiviral shRNA targeting RPA70, RPA32 or RPA14 alone or 24 h after irradiation (3 Gy; COMBO) or sham-irradiated and assessed for viability 72 h later. (E) Extreme limiting dilution assay (ELDA) of G01-GSCs transduced with lentiviral shRNA targeting of RPA70 (2 independent shRNAs) shows significantly impaired self-renewal of G01-GSCs after 10 days. Data are presented as mean ± SD. Statistical significance was tested using a two-way ANOVA with post-hoc Sidak's multiple comparisons test, where * p < 0.05, ** p < 0.01, *** p < 0.001. N = 3.

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