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Rabbit Polyclonal RPA32/RPA2 phospho S4 + S8 antibody. Suitable for IHC-P, IP, WB, ICC/IF and reacts with Mouse, Human samples. Immunogen corresponding to Synthetic Peptide within Human RPA2 phospho S4 + S8.

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Images

Immunocytochemistry/ Immunofluorescence - Anti-Rpa2 (phospho S4 + S8) antibody (AB264116), expandable thumbnail
  • Immunoprecipitation - Anti-Rpa2 (phospho S4 + S8) antibody (AB264116), expandable thumbnail
  • Western blot - Anti-Rpa2 (phospho S4 + S8) antibody (AB264116), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rpa2 (phospho S4 + S8) antibody (AB264116), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rpa2 (phospho S4 + S8) antibody (AB264116), expandable thumbnail

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7 - 8
Preservative: 0.09% Sodium azide
Constituents: Tris citrate/phosphate

Form

Liquid

Clonality

Polyclonal

Immunogen

  • Synthetic Peptide within Human RPA2 phospho S4 + S8. The exact immunogen used to generate this antibody is proprietary information. Database link P15927

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PIPWBICC/IF
Human
Tested
Tested
Tested
Tested
Mouse
Tested
Expected
Expected
Expected
Orangutan
Predicted
Predicted
Predicted
Predicted

Tested
Tested

Species

Mouse

Dilution info

1/500.00000 - 1/2000.00000

Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Species

Human

Dilution info

1/500.00000 - 1/2000.00000

Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Predicted
Predicted

Species

Orangutan

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

2.00000-10.00000 µg/mg of lysate

Notes

-

Expected
Expected

Species

Mouse

Dilution info

Use at an assay dependent concentration.

Notes

-

Predicted
Predicted

Species

Orangutan

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

1/2000.00000 - 1/10000.00000

Notes

-

Expected
Expected

Species

Mouse

Dilution info

Use at an assay dependent concentration.

Notes

-

Predicted
Predicted

Species

Orangutan

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

1/500.00000 - 1/5000.00000

Notes

-

Expected
Expected

Species

Mouse

Dilution info

Use at an assay dependent concentration.

Notes

-

Predicted
Predicted

Species

Orangutan

Dilution info

-

Notes

-

Target data

Function

As part of the heterotrimeric replication protein A complex (RPA/RP-A), binds and stabilizes single-stranded DNA intermediates that form during DNA replication or upon DNA stress. It prevents their reannealing and in parallel, recruits and activates different proteins and complexes involved in DNA metabolism. Thereby, it plays an essential role both in DNA replication and the cellular response to DNA damage. In the cellular response to DNA damage, the RPA complex controls DNA repair and DNA damage checkpoint activation. Through recruitment of ATRIP activates the ATR kinase a master regulator of the DNA damage response. It is required for the recruitment of the DNA double-strand break repair factors RAD51 and RAD52 to chromatin in response to DNA damage. Also recruits to sites of DNA damage proteins like XPA and XPG that are involved in nucleotide excision repair and is required for this mechanism of DNA repair. Also plays a role in base excision repair (BER) probably through interaction with UNG. Also recruits SMARCAL1/HARP, which is involved in replication fork restart, to sites of DNA damage. May also play a role in telomere maintenance. RPA stimulates 5'-3' helicase activity of BRIP1/FANCJ (PubMed:17596542).

Alternative names

Recommended products

Rabbit Polyclonal RPA32/RPA2 phospho S4 + S8 antibody. Suitable for IHC-P, IP, WB, ICC/IF and reacts with Mouse, Human samples. Immunogen corresponding to Synthetic Peptide within Human RPA2 phospho S4 + S8.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Polyclonal

Immunogen
  • Synthetic Peptide within Human RPA2 phospho S4 + S8. The exact immunogen used to generate this antibody is proprietary information. Database link P15927
Purification technique

Affinity purification Immunogen

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

Replication protein A2 (Rpa2) is an essential component of the DNA replication and repair processes. Also known as RPA32 or RFA2 Rpa2 is a subunit of the heterotrimeric complex known as Replication Protein A (RPA) which plays an important role in stabilizing single-stranded DNA. It has a molecular mass of approximately 32 kDa and is ubiquitously expressed across various cell types indicating its fundamental role in cellular maintenance and function. Rpa2 is primarily located in the nucleus where it contributes to the regulation of DNA metabolism.

Biological function summary

Rpa2 is part of the RPA complex that participates in DNA replication repair and recombination. This protein binds to single-stranded DNA protecting it from nucleases and preventing secondary structures that could form during replication or repair. In addition to its structural role Rpa2 facilitates the recruitment and stabilization of other essential proteins involved in DNA polymerization and repair ensuring the integrity of genetic material during cell division. The interaction of Rpa2 with various partners allows the cell to efficiently address DNA damage through coordinated signaling and repair mechanisms.

Pathways

Rpa2 is central to both the DNA replication and damage response pathways. It interacts closely with other proteins such as ATR and RAD51 which play important roles in the cellular response to DNA damage. In the replication pathway Rpa2 helps form pre-replication complexes by stabilizing and protecting the single-stranded DNA needed for the replication machinery. In the DNA damage response Rpa2 facilitates checkpoint signaling and repairs through its interactions with ATR which enables the cell to pause the cycle and perform necessary repairs for maintaining genomic stability.

Associated diseases and disorders

Disruptions in Rpa2 function correlate with genomic instability contributing to cancer and genetic disorders like xeroderma pigmentosum. Its role in maintaining DNA replication fidelity makes it a critical factor in cancer where mutations or dysregulations may promote tumorigenesis by allowing unchecked cell proliferation. Rpa2's involvement with proteins such as RAD51 is significant in homologous recombination repair a pathway frequently compromised in cancers leading to increased mutation rates and disease progression. Understanding Rpa2's role in these contexts can aid in the development of targeted therapies and enhance prognosis for affected individuals.

Product promise

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5 product images

  • Immunocytochemistry/ Immunofluorescence - Anti-Rpa2 (phospho S4 + S8) antibody (ab264116), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-Rpa2 (phospho S4 + S8) antibody (ab264116)

    Formaldehyde-fixed asynchronous HeLa cells. Untreated cells (left) and etoposide-treated cells (right) labeling Rpa2 using ab264116 at 1/1000 dilution (Red). A Red-fluorescent goat anti-rabbit IgG-heavy and light chain cross-adsorbed Antibody DyLight® 594 Conjugated used at a dilution of 1/100. Counterstained with an Alex Fluor® phalloidin (Green).

  • Immunoprecipitation - Anti-Rpa2 (phospho S4 + S8) antibody (ab264116), expandable thumbnail

    Immunoprecipitation - Anti-Rpa2 (phospho S4 + S8) antibody (ab264116)

    Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells treated with etoposide and/or Lambda PP or mock treated. RPA32 (S4/S8) was immunoprecipitated using a different rabbit anti-RPA32 antibody. For blotting immunoprecipitated Phospho RPA32 (S4/S8), ab264116 was used at 0.1 μg/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.
    Lane 1: ab264116 treated with etoposide and Lambda PP.
    Lane 2: ab264116 treated with etopside.
    Lane 3: Control IgG treated with etopside.

    All lanes: Immunoprecipitation - Anti-Rpa2 (phospho S4 + S8) antibody (ab264116)

    Predicted band size: 30 kDa

  • Western blot - Anti-Rpa2 (phospho S4 + S8) antibody (ab264116), expandable thumbnail

    Western blot - Anti-Rpa2 (phospho S4 + S8) antibody (ab264116)

    Detection: Chemiluminescence with an exposure time of 75 seconds.

    All lanes: Western blot - Anti-Rpa2 (phospho S4 + S8) antibody (ab264116) at 0.1 µg/mL

    Lane 1: HeLa cells treated with 100 µM etoposide (+) at 50 µg

    Lane 2: HeLa cells mock treated (-) at 50 µg

    Predicted band size: 30 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rpa2 (phospho S4 + S8) antibody (ab264116), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rpa2 (phospho S4 + S8) antibody (ab264116)

    Formalin-fixed, paraffin-embedded mouse colon carcinoma tissue stained for Rpa2 using ab264116 at 1/1000 dilution in immunohistochemical analysis. Detection: DAB

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rpa2 (phospho S4 + S8) antibody (ab264116), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rpa2 (phospho S4 + S8) antibody (ab264116)

    Formalin-fixed, paraffin-embedded human lung cancer tissue stained for Rpa2 using ab264116 at 1/1000 dilution in immunohistochemical analysis. Detection: DAB

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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