Rabbit Polyclonal RPA32/RPA2 phospho S4 + S8 antibody. Suitable for IHC-P, IP, WB, ICC/IF and reacts with Mouse, Human samples. Immunogen corresponding to Synthetic Peptide within Human RPA2 phospho S4 + S8.
IgG
Rabbit
pH: 7 - 8
Preservative: 0.09% Sodium azide
Constituents: Tris citrate/phosphate
Liquid
Polyclonal
IHC-P | IP | WB | ICC/IF | |
---|---|---|---|---|
Human | Tested | Tested | Tested | Tested |
Mouse | Tested | Expected | Expected | Expected |
Orangutan | Predicted | Predicted | Predicted | Predicted |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info 1/500.00000 - 1/2000.00000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Species Human | Dilution info 1/500.00000 - 1/2000.00000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Species | Dilution info | Notes |
---|---|---|
Species Orangutan | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 2.00000-10.00000 µg/mg of lysate | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Orangutan | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/2000.00000 - 1/10000.00000 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Orangutan | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/500.00000 - 1/5000.00000 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Orangutan | Dilution info - | Notes - |
As part of the heterotrimeric replication protein A complex (RPA/RP-A), binds and stabilizes single-stranded DNA intermediates that form during DNA replication or upon DNA stress. It prevents their reannealing and in parallel, recruits and activates different proteins and complexes involved in DNA metabolism. Thereby, it plays an essential role both in DNA replication and the cellular response to DNA damage. In the cellular response to DNA damage, the RPA complex controls DNA repair and DNA damage checkpoint activation. Through recruitment of ATRIP activates the ATR kinase a master regulator of the DNA damage response. It is required for the recruitment of the DNA double-strand break repair factors RAD51 and RAD52 to chromatin in response to DNA damage. Also recruits to sites of DNA damage proteins like XPA and XPG that are involved in nucleotide excision repair and is required for this mechanism of DNA repair. Also plays a role in base excision repair (BER) probably through interaction with UNG. Also recruits SMARCAL1/HARP, which is involved in replication fork restart, to sites of DNA damage. May also play a role in telomere maintenance. RPA stimulates 5'-3' helicase activity of BRIP1/FANCJ (PubMed:17596542).
REPA2, RPA32, RPA34, RPA34, RPA32, REPA2, RPA2, Replication protein A 32 kDa subunit, RP-A p32, Replication factor A protein 2, Replication protein A 34 kDa subunit, RF-A protein 2, RP-A p34
Rabbit Polyclonal RPA32/RPA2 phospho S4 + S8 antibody. Suitable for IHC-P, IP, WB, ICC/IF and reacts with Mouse, Human samples. Immunogen corresponding to Synthetic Peptide within Human RPA2 phospho S4 + S8.
IgG
Rabbit
pH: 7 - 8
Preservative: 0.09% Sodium azide
Constituents: Tris citrate/phosphate
Liquid
Polyclonal
Affinity purification Immunogen
Blue Ice
1-2 weeks
+4°C
-20°C
Upon delivery aliquot
Avoid freeze / thaw cycle
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This supplementary information is collated from multiple sources and compiled automatically.
Replication protein A2 (Rpa2) is an essential component of the DNA replication and repair processes. Also known as RPA32 or RFA2 Rpa2 is a subunit of the heterotrimeric complex known as Replication Protein A (RPA) which plays an important role in stabilizing single-stranded DNA. It has a molecular mass of approximately 32 kDa and is ubiquitously expressed across various cell types indicating its fundamental role in cellular maintenance and function. Rpa2 is primarily located in the nucleus where it contributes to the regulation of DNA metabolism.
Rpa2 is part of the RPA complex that participates in DNA replication repair and recombination. This protein binds to single-stranded DNA protecting it from nucleases and preventing secondary structures that could form during replication or repair. In addition to its structural role Rpa2 facilitates the recruitment and stabilization of other essential proteins involved in DNA polymerization and repair ensuring the integrity of genetic material during cell division. The interaction of Rpa2 with various partners allows the cell to efficiently address DNA damage through coordinated signaling and repair mechanisms.
Rpa2 is central to both the DNA replication and damage response pathways. It interacts closely with other proteins such as ATR and RAD51 which play important roles in the cellular response to DNA damage. In the replication pathway Rpa2 helps form pre-replication complexes by stabilizing and protecting the single-stranded DNA needed for the replication machinery. In the DNA damage response Rpa2 facilitates checkpoint signaling and repairs through its interactions with ATR which enables the cell to pause the cycle and perform necessary repairs for maintaining genomic stability.
Disruptions in Rpa2 function correlate with genomic instability contributing to cancer and genetic disorders like xeroderma pigmentosum. Its role in maintaining DNA replication fidelity makes it a critical factor in cancer where mutations or dysregulations may promote tumorigenesis by allowing unchecked cell proliferation. Rpa2's involvement with proteins such as RAD51 is significant in homologous recombination repair a pathway frequently compromised in cancers leading to increased mutation rates and disease progression. Understanding Rpa2's role in these contexts can aid in the development of targeted therapies and enhance prognosis for affected individuals.
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Formaldehyde-fixed asynchronous HeLa cells. Untreated cells (left) and etoposide-treated cells (right) labeling Rpa2 using ab264116 at 1/1000 dilution (Red). A Red-fluorescent goat anti-rabbit IgG-heavy and light chain cross-adsorbed Antibody DyLight® 594 Conjugated used at a dilution of 1/100. Counterstained with an Alex Fluor® phalloidin (Green).
Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells treated with etoposide and/or Lambda PP or mock treated. RPA32 (S4/S8) was immunoprecipitated using a different rabbit anti-RPA32 antibody. For blotting immunoprecipitated Phospho RPA32 (S4/S8), ab264116 was used at 0.1 μg/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.
Lane 1: ab264116 treated with etoposide and Lambda PP.
Lane 2: ab264116 treated with etopside.
Lane 3: Control IgG treated with etopside.
All lanes: Immunoprecipitation - Anti-Rpa2 (phospho S4 + S8) antibody (ab264116)
Predicted band size: 30 kDa
Detection: Chemiluminescence with an exposure time of 75 seconds.
All lanes: Western blot - Anti-Rpa2 (phospho S4 + S8) antibody (ab264116) at 0.1 µg/mL
Lane 1: HeLa cells treated with 100 µM etoposide (+) at 50 µg
Lane 2: HeLa cells mock treated (-) at 50 µg
Predicted band size: 30 kDa
Formalin-fixed, paraffin-embedded mouse colon carcinoma tissue stained for Rpa2 using ab264116 at 1/1000 dilution in immunohistochemical analysis. Detection: DAB
Formalin-fixed, paraffin-embedded human lung cancer tissue stained for Rpa2 using ab264116 at 1/1000 dilution in immunohistochemical analysis. Detection: DAB
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