JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB264116

Anti-Rpa2 (phospho S4 + S8) antibody

Be the first to review this product! Submit a review

|

(1 Publication)

Rabbit Polyclonal RPA32/RPA2 phospho S4 + S8 antibody. Suitable for IHC-P, IP, WB, ICC/IF and reacts with Mouse, Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human RPA2 phospho S4 + S8.

View Alternative Names

REPA2, RPA32, RPA34, RPA2, Replication protein A 32 kDa subunit, RP-A p32, Replication factor A protein 2, Replication protein A 34 kDa subunit, RF-A protein 2, RP-A p34

5 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rpa2 (phospho S4 + S8) antibody (AB264116)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rpa2 (phospho S4 + S8) antibody (AB264116)

Formalin-fixed, paraffin-embedded human lung cancer tissue stained for Rpa2 using ab264116 at 1/1000 dilution in immunohistochemical analysis. Detection : DAB

Immunocytochemistry/ Immunofluorescence - Anti-Rpa2 (phospho S4 + S8) antibody (AB264116)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Rpa2 (phospho S4 + S8) antibody (AB264116)

Formaldehyde-fixed asynchronous HeLa cells. Untreated cells (left) and etoposide-treated cells (right) labeling Rpa2 using ab264116 at 1/1000 dilution (Red). A Red-fluorescent goat anti-rabbit IgG-heavy and light chain cross-adsorbed Antibody DyLight® 594 Conjugated used at a dilution of 1/100. Counterstained with an Alex Fluor® phalloidin (Green).

Immunoprecipitation - Anti-Rpa2 (phospho S4 + S8) antibody (AB264116)
  • IP

Supplier Data

Immunoprecipitation - Anti-Rpa2 (phospho S4 + S8) antibody (AB264116)

Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells treated with etoposide and/or Lambda PP or mock treated. RPA32 (S4/S8) was immunoprecipitated using a different rabbit anti-RPA32 antibody. For blotting immunoprecipitated Phospho RPA32 (S4/S8), ab264116 was used at 0.1 μg/ml. Detection : Chemiluminescence with an exposure time of 75 seconds.
Lane 1 : ab264116 treated with etoposide and Lambda PP.
Lane 2 : ab264116 treated with etopside.
Lane 3 : Control IgG treated with etopside.

All lanes:

Immunoprecipitation - Anti-Rpa2 (phospho S4 + S8) antibody (ab264116)

Predicted band size: 30 kDa

false

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rpa2 (phospho S4 + S8) antibody (AB264116)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rpa2 (phospho S4 + S8) antibody (AB264116)

Formalin-fixed, paraffin-embedded mouse colon carcinoma tissue stained for Rpa2 using ab264116 at 1/1000 dilution in immunohistochemical analysis. Detection : DAB

Western blot - Anti-Rpa2 (phospho S4 + S8) antibody (AB264116)
  • WB

Supplier Data

Western blot - Anti-Rpa2 (phospho S4 + S8) antibody (AB264116)

Detection : Chemiluminescence with an exposure time of 75 seconds.

All lanes:

Western blot - Anti-Rpa2 (phospho S4 + S8) antibody (ab264116) at 0.1 µg/mL

Lane 1:

HeLa cells treated with 100 µM etoposide (+) at 50 µg

Lane 2:

HeLa cells mock treated (-) at 50 µg

Predicted band size: 30 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

ICC/IF, IHC-P, WB, IP

applications

Immunogen

Synthetic Peptide within Human RPA2 phospho S4 + S8. The exact immunogen used to generate this antibody is proprietary information.

P15927

style
left-column

Complementary Products

Primary Antibodies

AB133534

Anti-Rad51 antibody [EPR4030(3)]

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rad51 antibody [EPR4030(3)] (AB133534)

  • 4
  • 15
Primary Antibodies

AB6326

Anti-BrdU antibody [BU1/75 (ICR1)] - Proliferation Marker

Flow Cytometry (Intracellular) - Anti-BrdU antibody [BU1/75 (ICR1)] - Proliferation Marker (AB6326)

  • 5
  • 104
Primary Antibodies

AB81299

Anti-gamma H2A.X (phospho S139) antibody [EP854(2)Y]

BOND RX™ Validated|20ul selling size|RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Anti-gamma H2A.X (phospho S139) antibody [EP854(2)Y] (AB81299)

  • 4
  • 17
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/500 - 1/2000", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-10 µg/mg of lysate", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/500 - 1/5000", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/500 - 1/2000", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Orangutan": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
style
left-column
style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: Tris citrate/phosphate
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Do Not Freeze
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Replication protein A2 (Rpa2) is an essential component of the DNA replication and repair processes. Also known as RPA32 or RFA2 Rpa2 is a subunit of the heterotrimeric complex known as Replication Protein A (RPA) which plays an important role in stabilizing single-stranded DNA. It has a molecular mass of approximately 32 kDa and is ubiquitously expressed across various cell types indicating its fundamental role in cellular maintenance and function. Rpa2 is primarily located in the nucleus where it contributes to the regulation of DNA metabolism.
Biological function summary

Rpa2 is part of the RPA complex that participates in DNA replication repair and recombination. This protein binds to single-stranded DNA protecting it from nucleases and preventing secondary structures that could form during replication or repair. In addition to its structural role Rpa2 facilitates the recruitment and stabilization of other essential proteins involved in DNA polymerization and repair ensuring the integrity of genetic material during cell division. The interaction of Rpa2 with various partners allows the cell to efficiently address DNA damage through coordinated signaling and repair mechanisms.

Pathways

Rpa2 is central to both the DNA replication and damage response pathways. It interacts closely with other proteins such as ATR and RAD51 which play important roles in the cellular response to DNA damage. In the replication pathway Rpa2 helps form pre-replication complexes by stabilizing and protecting the single-stranded DNA needed for the replication machinery. In the DNA damage response Rpa2 facilitates checkpoint signaling and repairs through its interactions with ATR which enables the cell to pause the cycle and perform necessary repairs for maintaining genomic stability.

Disruptions in Rpa2 function correlate with genomic instability contributing to cancer and genetic disorders like xeroderma pigmentosum. Its role in maintaining DNA replication fidelity makes it a critical factor in cancer where mutations or dysregulations may promote tumorigenesis by allowing unchecked cell proliferation. Rpa2's involvement with proteins such as RAD51 is significant in homologous recombination repair a pathway frequently compromised in cancers leading to increased mutation rates and disease progression. Understanding Rpa2's role in these contexts can aid in the development of targeted therapies and enhance prognosis for affected individuals.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

As part of the heterotrimeric replication protein A complex (RPA/RP-A), binds and stabilizes single-stranded DNA intermediates that form during DNA replication or upon DNA stress. It prevents their reannealing and in parallel, recruits and activates different proteins and complexes involved in DNA metabolism. Thereby, it plays an essential role both in DNA replication and the cellular response to DNA damage. In the cellular response to DNA damage, the RPA complex controls DNA repair and DNA damage checkpoint activation. Through recruitment of ATRIP activates the ATR kinase a master regulator of the DNA damage response. It is required for the recruitment of the DNA double-strand break repair factors RAD51 and RAD52 to chromatin in response to DNA damage. Also recruits to sites of DNA damage proteins like XPA and XPG that are involved in nucleotide excision repair and is required for this mechanism of DNA repair. Also plays a role in base excision repair (BER) probably through interaction with UNG. Also recruits SMARCAL1/HARP, which is involved in replication fork restart, to sites of DNA damage. May also play a role in telomere maintenance. RPA stimulates 5'-3' helicase activity of BRIP1/FANCJ (PubMed : 17596542).
See full target information RPA2 phospho S4 + S8
style
left-column

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Journal of cellular and molecular medicine 21:3066-3075 PubMed28580627

2017

Age-related modulation of angiogenesis-regulating factors in the swine meniscus.

Applications

Unspecified application

Species

Unspecified reactive species

Alessia Di Giancamillo,Daniela Deponti,Silvia Modina,Irene Tessaro,Cinzia Domeneghini,Giuseppe Maria Peretti
View all publications
style
left-column
style
left-column
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com