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Rat Recombinant Monoclonal RPA32/RPA2 antibody. Suitable for I-ELISA, WB, ICC/IF and reacts with Human, Mouse, Rat samples. Cited in 1 publication.

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Images

Western blot - Anti-RPA32/RPA2 antibody [4E4] (AB252861), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-RPA32/RPA2 antibody [4E4] (AB252861), expandable thumbnail
  • Western blot - Anti-RPA32/RPA2 antibody [4E4] (AB252861), expandable thumbnail
  • Western blot - Anti-RPA32/RPA2 antibody [4E4] (AB252861), expandable thumbnail
  • Indirect ELISA - Anti-RPA32/RPA2 antibody [4E4] (AB252861), expandable thumbnail

Publications

Key facts

Isotype

IgG1

Host species

Rat

Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
I-ELISAWBICC/IF
Human
Tested
Tested
Tested
Mouse
Expected
Tested
Expected
Rat
Expected
Tested
Expected

Tested
Tested

Species

Human

Dilution info

0.20000-5.00000 µg/mL

Notes

-

Expected
Expected

Species

Mouse, Rat

Dilution info

Use at an assay dependent concentration.

Notes

-

Tested
Tested

Species

Mouse

Dilution info

1/1000

Notes

-

Species

Rat

Dilution info

1/1000

Notes

-

Species

Human

Dilution info

1/1000

Notes

-

Tested
Tested

Species

Human

Dilution info

1/50

Notes

-

Expected
Expected

Species

Mouse, Rat

Dilution info

Use at an assay dependent concentration.

Notes

-

Associated Products

Select an associated product type

6 products for Alternative Product

Target data

Function

As part of the heterotrimeric replication protein A complex (RPA/RP-A), binds and stabilizes single-stranded DNA intermediates that form during DNA replication or upon DNA stress. It prevents their reannealing and in parallel, recruits and activates different proteins and complexes involved in DNA metabolism. Thereby, it plays an essential role both in DNA replication and the cellular response to DNA damage. In the cellular response to DNA damage, the RPA complex controls DNA repair and DNA damage checkpoint activation. Through recruitment of ATRIP activates the ATR kinase a master regulator of the DNA damage response. It is required for the recruitment of the DNA double-strand break repair factors RAD51 and RAD52 to chromatin in response to DNA damage. Also recruits to sites of DNA damage proteins like XPA and XPG that are involved in nucleotide excision repair and is required for this mechanism of DNA repair. Also plays a role in base excision repair (BER) probably through interaction with UNG. Also recruits SMARCAL1/HARP, which is involved in replication fork restart, to sites of DNA damage. May also play a role in telomere maintenance. RPA stimulates 5'-3' helicase activity of BRIP1/FANCJ (PubMed:17596542).

Alternative names

Recommended products

Rat Recombinant Monoclonal RPA32/RPA2 antibody. Suitable for I-ELISA, WB, ICC/IF and reacts with Human, Mouse, Rat samples. Cited in 1 publication.

Key facts

Isotype

IgG1

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

4E4

Purification technique

Affinity purification Protein A

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

5 product images

  • Western blot - Anti-RPA32/RPA2 antibody [4E4] (ab252861), expandable thumbnail

    Western blot - Anti-RPA32/RPA2 antibody [4E4] (ab252861)

    Blocking buffer: 5% NFDM/TBST

    All lanes: Western blot - Anti-RPA32/RPA2 antibody [4E4] (ab252861) at 1/1000 dilution

    Lane 1: HeLa (human cervix adenocarcinoma epithelial cell ), whole cell lysate at 20 µg

    Lane 2: HUVEC (human umbilical vein endothelial cell ), whole cell lysate at 20 µg

    Lane 3: U-2 OS (human bone osteosarcoma epithelial cell), whole cell lysate at 20 µg

    Lane 4: C6 (rat glial tumor glial cell), whole cell lysate at 20 µg

    Lane 5: NIH/3T3 (mouse embryonic fibroblast), whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rat IgG H&L (HRP) (Goat Anti-Rat IgG H&L (HRP) ab205720) at 1/5000 dilution

    Predicted band size: 29 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-RPA32/RPA2 antibody [4E4] (ab252861), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-RPA32/RPA2 antibody [4E4] (ab252861)

    Immunofluorescence analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling RPA32/RPA2 (green) with ab252861 at 1/50 dilution, followed by secondary antibody (Goat Anti-Rat IgG H&L (Alexa Fluor® 488) ab150157) at 1:1000 (2μg/mL). Confocal image showing nuclear staining in HeLa cell line.

    Counterstain: DAPI (blue) - Anti-beta IV Tubulin antibody [EPR16775] ab179504 (YCA-R16775) Anti-beta IV Tubulin at 1:200 (2.5 μg/mL).

    Counterstain Secondary antibody: Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) ab150080 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594-Red) at 1:500 (20μg/mL).

    Secondary antibody only control: Used PBS instead of primary antibody, followed by Goat Anti-Rat IgG H&L (Alexa Fluor® 488) ab150157 Goat Anti-Rat IgG H&L (Alexa Fluor® 488) secondary.

  • Western blot - Anti-RPA32/RPA2 antibody [4E4] (ab252861), expandable thumbnail

    Western blot - Anti-RPA32/RPA2 antibody [4E4] (ab252861)

    Blocking buffer: 5% NFDM/TBST

    Exposure time: 5 seconds

    All lanes: Western blot - Anti-RPA32/RPA2 antibody [4E4] (ab252861) at 1/1000 dilution

    Lane 1: Human brain tissue lysate at 20 µg

    Lane 2: Human heart tissue lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rat IgG H&L (HRP) (Goat Anti-Rat IgG H&L (HRP) ab205720) at 1/5000 dilution

    Predicted band size: 29 kDa

  • Western blot - Anti-RPA32/RPA2 antibody [4E4] (ab252861), expandable thumbnail

    Western blot - Anti-RPA32/RPA2 antibody [4E4] (ab252861)

    Blocking buffer: 5% NFDM/TBST

    Exposure time:

    lane 1: 5.5 seconds
    lane 2: 3 seconds

    All lanes: Western blot - Anti-RPA32/RPA2 antibody [4E4] (ab252861) at 1/1000 dilution

    Lane 1: Mouse brain tissue lysate at 20 µg

    Lane 2: Rat brain tissue lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rat IgG H&L (HRP) (Goat Anti-Rat IgG H&L (HRP) ab205720) at 1/5000 dilution

    Predicted band size: 29 kDa

  • Indirect ELISA - Anti-RPA32/RPA2 antibody [4E4] (ab252861), expandable thumbnail

    Indirect ELISA - Anti-RPA32/RPA2 antibody [4E4] (ab252861)

    Human RPA32/RPA2= 1μg/mL

    ab252861 used at 0-5000 ng/mL.

    Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rat IgG (H+L) used at 1:1000.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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