Rat Recombinant Monoclonal RPA32/RPA2 antibody. Suitable for I-ELISA, WB, ICC/IF and reacts with Human, Mouse, Rat samples. Cited in 1 publication.
IgG1
Rat
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Liquid
Monoclonal
I-ELISA | WB | ICC/IF | |
---|---|---|---|
Human | Tested | Tested | Tested |
Mouse | Expected | Tested | Expected |
Rat | Expected | Tested | Expected |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 0.20000-5.00000 µg/mL | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info 1/1000 | Notes - |
Species Rat | Dilution info 1/1000 | Notes - |
Species Human | Dilution info 1/1000 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/50 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Select an associated product type
As part of the heterotrimeric replication protein A complex (RPA/RP-A), binds and stabilizes single-stranded DNA intermediates that form during DNA replication or upon DNA stress. It prevents their reannealing and in parallel, recruits and activates different proteins and complexes involved in DNA metabolism. Thereby, it plays an essential role both in DNA replication and the cellular response to DNA damage. In the cellular response to DNA damage, the RPA complex controls DNA repair and DNA damage checkpoint activation. Through recruitment of ATRIP activates the ATR kinase a master regulator of the DNA damage response. It is required for the recruitment of the DNA double-strand break repair factors RAD51 and RAD52 to chromatin in response to DNA damage. Also recruits to sites of DNA damage proteins like XPA and XPG that are involved in nucleotide excision repair and is required for this mechanism of DNA repair. Also plays a role in base excision repair (BER) probably through interaction with UNG. Also recruits SMARCAL1/HARP, which is involved in replication fork restart, to sites of DNA damage. May also play a role in telomere maintenance. RPA stimulates 5'-3' helicase activity of BRIP1/FANCJ (PubMed:17596542).
REPA2, RPA32, RPA34, Replication protein A 32 kDa subunit, RP-A p32, Replication factor A protein 2, Replication protein A 34 kDa subunit, RF-A protein 2, RP-A p34
Rat Recombinant Monoclonal RPA32/RPA2 antibody. Suitable for I-ELISA, WB, ICC/IF and reacts with Human, Mouse, Rat samples. Cited in 1 publication.
IgG1
Rat
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Liquid
Monoclonal
4E4
Affinity purification Protein A
Blue Ice
1-2 weeks
+4°C
-20°C
Upon delivery aliquot
Avoid freeze / thaw cycle
This product is a recombinant monoclonal antibody, which offers several advantages including:
For more information, read more on recombinant antibodies.
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com
We have tested this species and application combination and it works. It is covered by our product promise.
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
Blocking buffer: 5% NFDM/TBST
All lanes: Western blot - Anti-RPA32/RPA2 antibody [4E4] (ab252861) at 1/1000 dilution
Lane 1: HeLa (human cervix adenocarcinoma epithelial cell ), whole cell lysate at 20 µg
Lane 2: HUVEC (human umbilical vein endothelial cell ), whole cell lysate at 20 µg
Lane 3: U-2 OS (human bone osteosarcoma epithelial cell), whole cell lysate at 20 µg
Lane 4: C6 (rat glial tumor glial cell), whole cell lysate at 20 µg
Lane 5: NIH/3T3 (mouse embryonic fibroblast), whole cell lysate at 20 µg
All lanes: Western blot - Goat Anti-Rat IgG H&L (HRP) (Goat Anti-Rat IgG H&L (HRP) ab205720) at 1/5000 dilution
Predicted band size: 29 kDa
Immunofluorescence analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling RPA32/RPA2 (green) with ab252861 at 1/50 dilution, followed by secondary antibody (Goat Anti-Rat IgG H&L (Alexa Fluor® 488) ab150157) at 1:1000 (2μg/mL). Confocal image showing nuclear staining in HeLa cell line.
Counterstain: DAPI (blue) - Anti-beta IV Tubulin antibody [EPR16775] ab179504 (YCA-R16775) Anti-beta IV Tubulin at 1:200 (2.5 μg/mL).
Counterstain Secondary antibody: Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) ab150080 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594-Red) at 1:500 (20μg/mL).
Secondary antibody only control: Used PBS instead of primary antibody, followed by Goat Anti-Rat IgG H&L (Alexa Fluor® 488) ab150157 Goat Anti-Rat IgG H&L (Alexa Fluor® 488) secondary.
Blocking buffer: 5% NFDM/TBST
Exposure time: 5 seconds
All lanes: Western blot - Anti-RPA32/RPA2 antibody [4E4] (ab252861) at 1/1000 dilution
Lane 1: Human brain tissue lysate at 20 µg
Lane 2: Human heart tissue lysate at 20 µg
All lanes: Western blot - Goat Anti-Rat IgG H&L (HRP) (Goat Anti-Rat IgG H&L (HRP) ab205720) at 1/5000 dilution
Predicted band size: 29 kDa
Blocking buffer: 5% NFDM/TBST
Exposure time:
lane 1: 5.5 seconds
lane 2: 3 seconds
All lanes: Western blot - Anti-RPA32/RPA2 antibody [4E4] (ab252861) at 1/1000 dilution
Lane 1: Mouse brain tissue lysate at 20 µg
Lane 2: Rat brain tissue lysate at 20 µg
All lanes: Western blot - Goat Anti-Rat IgG H&L (HRP) (Goat Anti-Rat IgG H&L (HRP) ab205720) at 1/5000 dilution
Predicted band size: 29 kDa
Human RPA32/RPA2= 1μg/mL
ab252861 used at 0-5000 ng/mL.
Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rat IgG (H+L) used at 1:1000.
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com