AB255855

Anti-RPA32/RPA2 antibody [4E4] - BSA and Azide free

Recombinant
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Rat Recombinant Monoclonal RPA32/RPA2 antibody. Carrier free. Suitable for I-ELISA, WB, ICC/IF and reacts with Human, Mouse, Rat samples.

View Alternative Names

REPA2, RPA32, RPA34, RPA2, Replication protein A 32 kDa subunit, RP-A p32, Replication factor A protein 2, Replication protein A 34 kDa subunit, RF-A protein 2, RP-A p34

2 Images

Immunocytochemistry/ Immunofluorescence - Anti-RPA32/RPA2 antibody [4E4] - BSA and Azide free (AB255855)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-RPA32/RPA2 antibody [4E4] - BSA and Azide free (AB255855)

Immunofluorescence analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling RPA32/RPA2 (green) with ab252861 at 1/50 dilution, followed by secondary antibody (ab150157) at 1 : 1000 (2μg/mL). Confocal image showing nuclear staining in HeLa cell line.

Counterstain : DAPI (blue) - ab179504 (YCA-R16775) Anti-beta IV Tubulin at 1 : 200 (2.5 μg/mL).

Counterstain Secondary antibody : ab150080 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594-Red) at 1 : 500 (20μg/mL).

Secondary antibody only control : Used PBS instead of primary antibody, followed by ab150157 Goat Anti-Rat IgG H&L (Alexa Fluor® 488) secondary.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, and sodium azide (ab252861).

Indirect ELISA - Anti-RPA32/RPA2 antibody [4E4] - BSA and Azide free (AB255855)

I-ELISA

Supplier Data

Indirect ELISA - Anti-RPA32/RPA2 antibody [4E4] - BSA and Azide free (AB255855)

Human RPA32/RPA2= 1μg/mL

ab252861 used at 0-5000 ng/mL.

Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rat IgG (H+L) used at 1 : 1000.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, and sodium azide (ab252861).

Unconjugated

Anti-RPA32/RPA2 antibody [4E4]

Key facts

Host species

Rat

Clonality

Monoclonal

Clone number

4E4

Isotype

IgG1

Carrier free

Yes

Reacts with

Human, Mouse, Rat

Applications

ICC/IF, I-ELISA, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IELISA" : {"fullname" : "Indirect ELISA", "shortname":"I-ELISA"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IELISA-species-checked": "testedAndGuaranteed", "IELISA-species-dilution-info": "", "IELISA-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "IELISA-species-checked": "guaranteed", "IELISA-species-dilution-info": "", "IELISA-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IELISA-species-checked": "guaranteed", "IELISA-species-dilution-info": "", "IELISA-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Product details

ab255855 is the carrier-free version of ab252861.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

As part of the heterotrimeric replication protein A complex (RPA/RP-A), binds and stabilizes single-stranded DNA intermediates that form during DNA replication or upon DNA stress. It prevents their reannealing and in parallel, recruits and activates different proteins and complexes involved in DNA metabolism. Thereby, it plays an essential role both in DNA replication and the cellular response to DNA damage. In the cellular response to DNA damage, the RPA complex controls DNA repair and DNA damage checkpoint activation. Through recruitment of ATRIP activates the ATR kinase a master regulator of the DNA damage response. It is required for the recruitment of the DNA double-strand break repair factors RAD51 and RAD52 to chromatin in response to DNA damage. Also recruits to sites of DNA damage proteins like XPA and XPG that are involved in nucleotide excision repair and is required for this mechanism of DNA repair. Also plays a role in base excision repair (BER) probably through interaction with UNG. Also recruits SMARCAL1/HARP, which is involved in replication fork restart, to sites of DNA damage. May also play a role in telomere maintenance. RPA stimulates 5'-3' helicase activity of BRIP1/FANCJ (PubMed : 17596542).

See full target information RPA2

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-RPA32/RPA2 antibody [4E4] - BSA and Azide free

Immunocytochemistry/ Immunofluorescence - Anti-RPA32/RPA2 antibody [4E4] - BSA and Azide free (AB255855)

Indirect ELISA - Anti-RPA32/RPA2 antibody [4E4] - BSA and Azide free (AB255855)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Product protocols

Target data

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