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Anti-RPA32/RPA2 antibody [9H8] ab2175 is a mouse monoclonal antibody that is used in RPA32/RPA2 western blotting, IHC and flow cytometry. Suitable for human samples.

- Antibody clone 9H8 is the most widely used clone for RPA32/RPA2 on the market and is cited in >370 publications


Images

Flow Cytometry - Anti-RPA32/RPA2 antibody [9H8] (AB2175), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPA32/RPA2 antibody [9H8] (AB2175), expandable thumbnail
  • Flow Cytometry - Anti-RPA32/RPA2 antibody [9H8] (AB2175), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPA32/RPA2 antibody [9H8] (AB2175), expandable thumbnail
  • Western blot - Anti-RPA32/RPA2 antibody [9H8] (AB2175), expandable thumbnail

Publications

Key facts

Isotype

IgG1

Host species

Mouse

Storage buffer

pH: 7.3 - 7.5
Preservative: 0.05% Sodium azide
Constituents: 1% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • Native Full Length Protein corresponding to Human RPA2. Database link P15927

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PFlow CytWB
Human
Tested
Tested
Tested

Tested
Tested

Species

Human

Dilution info

1/50.00000 - 1/100.00000

Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Tested
Tested

Species

Human

Dilution info

1 µg for 106 Cells

Notes

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

Tested
Tested

Species

Human

Dilution info

-

Notes

-

Associated Products

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Target data

Function

As part of the heterotrimeric replication protein A complex (RPA/RP-A), binds and stabilizes single-stranded DNA intermediates that form during DNA replication or upon DNA stress. It prevents their reannealing and in parallel, recruits and activates different proteins and complexes involved in DNA metabolism. Thereby, it plays an essential role both in DNA replication and the cellular response to DNA damage. In the cellular response to DNA damage, the RPA complex controls DNA repair and DNA damage checkpoint activation. Through recruitment of ATRIP activates the ATR kinase a master regulator of the DNA damage response. It is required for the recruitment of the DNA double-strand break repair factors RAD51 and RAD52 to chromatin in response to DNA damage. Also recruits to sites of DNA damage proteins like XPA and XPG that are involved in nucleotide excision repair and is required for this mechanism of DNA repair. Also plays a role in base excision repair (BER) probably through interaction with UNG. Also recruits SMARCAL1/HARP, which is involved in replication fork restart, to sites of DNA damage. May also play a role in telomere maintenance. RPA stimulates 5'-3' helicase activity of BRIP1/FANCJ (PubMed:17596542).

Alternative names

Recommended products

Anti-RPA32/RPA2 antibody [9H8] ab2175 is a mouse monoclonal antibody that is used in RPA32/RPA2 western blotting, IHC and flow cytometry. Suitable for human samples.

- Antibody clone 9H8 is the most widely used clone for RPA32/RPA2 on the market and is cited in >370 publications

Key facts

Isotype

IgG1

Form

Liquid

Clonality

Monoclonal

Immunogen
  • Native Full Length Protein corresponding to Human RPA2. Database link P15927
Clone number

9H8

Purity

IgG fraction

Light chain type

kappa

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
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5 product images

  • Flow Cytometry - Anti-RPA32/RPA2 antibody [9H8] (ab2175), expandable thumbnail
    This image is courtesy of an anonymous customer review

    Flow Cytometry - Anti-RPA32/RPA2 antibody [9H8] (ab2175)

    Anti-RPA/32/RPA2 antibody [9H8] (ab2175) is used to measure DNA Double-Stranded Breaks resection in U2OS cells +/- Camptotechin (CPT).

    ab2175 is used at 1/200 dilution in 1xPBS + 0.2% Triton X-100 for 1 hour at 25°C.

    Secondary antibody: anti-mouse Molecular Probes Alexa Fluor® 488 Conjugated at 1/200 dilution.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPA32/RPA2 antibody [9H8] (ab2175), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPA32/RPA2 antibody [9H8] (ab2175)

    Formalin-fixed, paraffin-embeded human tonsil tissue stained for RPA32/RPA2 using ab2175 at 1/50 dilution in immunohistochemical analysis. Antigen retrieval with citrate buffer pH 6.0

  • Flow Cytometry - Anti-RPA32/RPA2 antibody [9H8] (ab2175), expandable thumbnail

    Flow Cytometry - Anti-RPA32/RPA2 antibody [9H8] (ab2175)

    Overlay histogram showing HeLa cells stained with ab2175 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab2175, 1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (Goat Anti-Mouse IgG H&L (DyLight® 488) preadsorbed ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (Mouse IgG1, Kappa Monoclonal [B11/6] - Isotype Control ab91353, 2μg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPA32/RPA2 antibody [9H8] (ab2175), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPA32/RPA2 antibody [9H8] (ab2175)

    Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human tonsil tissue, staining RPA32/RPA2 with ab2175. Staining was detected using DAB.

  • Western blot - Anti-RPA32/RPA2 antibody [9H8] (ab2175), expandable thumbnail

    Western blot - Anti-RPA32/RPA2 antibody [9H8] (ab2175)

    All lanes: Western blot - Anti-RPA32/RPA2 antibody [9H8] (ab2175) at 5 µg/mL

    Lane 1: TE 671 (Human Rhabdomyosarcoma) Whole Cell Lysate at 10 µg

    Lane 2: SK N BE (Human neuroblastoma) Whole Cell Lysate at 10 µg

    Lane 3: HeLa (Human epithelial carcinoma cell line) Nuclear Lysate at 10 µg

    Secondary

    All lanes: Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Predicted band size: 29 kDa

    Observed band size: 32 kDa

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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