Anti-RPA32/RPA2 antibody (ab10359) is a rabbit polyclonal antibody that is used to detect RPA32/RPA2 in Western Blot, IP, IHC-P. Suitable for Human, Mouse samples.
- Over 10 publications
- Trusted since 2004
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- Genome research 33:2018-20272023Characterization of meiotic recombination intermediates through gene knockouts in founder hybrid mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBenjamin Davies,Gang Zhang,Daniela Moralli,Samy Alghadban,Daniel Biggs,Chris Preece,Peter Donnelly,Anjali Gupta HinchPubMed 37977820
- Frontiers in cell and developmental biology 11:11476102023Divergent patterns of meiotic double strand breaks and synapsis initiation dynamics suggest an evolutionary shift in the meiosis program between American and Australian marsupials.Applications:Unspecified applicationReactive species:Unspecified reactive speciesF Javier Valero-Regalón,Mireia Solé,Pablo López-Jiménez,María Valerio-de Arana,Marta Martín-Ruiz,Roberto de la Fuente,Laia Marín-Gual,Marilyn B Renfree,Geoff Shaw,Soledad Berríos,Raúl Fernández-Donoso,Paul D Waters,Aurora Ruiz-Herrera,Rocío Gómez,Jesús PagePubMed 37181752
- Cell reports 42:1120622023GEN1 promotes common fragile site expression.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAnaid Benitez,Marie Sebald,Radhakrishnan Kanagaraj,Monica C Rodrigo-Brenni,Ying Wai Chan,Chih-Chao Liang,Stephen C WestPubMed 36729836
- eLife 11:2022DNA-PK promotes DNA end resection at DNA double strand breaks in G cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesFaith C Fowler,Bo-Ruei Chen,Nicholas Zolnerowich,Wei Wu,Raphael Pavani,Jacob Paiano,Chelsea Peart,Zulong Chen,André Nussenzweig,Barry P Sleckman,Jessica K TylerPubMed 35575473
- Genes & development 35:1356-13672021Role of 53BP1 in end protection and DNA synthesis at DNA breaks.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJacob Paiano,Nicholas Zolnerowich,Wei Wu,Raphael Pavani,Chen Wang,Hongzhi Li,Li Zheng,Binghui Shen,Barry P Sleckman,Bo-Ruei Chen,André NussenzweigPubMed 34503990
- Molecular human reproduction 27:2021Integrated stress response control of granulosa cell translation and proliferation during normal ovarian follicle development.Applications:Unspecified applicationReactive species:Unspecified reactive speciesEvelyn Llerena Cari,Synneva Hagen-Lillevik,Asma Giornazi,Miriam Post,Anton A Komar,Leslie Appiah,Benjamin Bitler,Alex J Polotsky,Nanette Santoro,Jeffrey Kieft,Kent Lai,Joshua JohnsonPubMed 34314477
- American journal of translational research 13:601-6162021LACTB induced apoptosis of oxaliplatin-resistant gastric cancer through regulating autophagy-mediated mitochondrial apoptosis pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesFang Yang,Zhiqiang Yan,Wei Nie,Xingzhen Cheng,Zeying Liu,Wei Wang,Chunyan Shao,Gui Fu,Yanni YuPubMed 33594312
- Nature communications 12:5852021The Bloom syndrome complex senses RPA-coated single-stranded DNA to restart stalled replication forks.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAnn-Marie K Shorrocks et. al.PubMed 33500419
- eLife 9:2020ZCWPW1 is recruited to recombination hotspots by PRDM9 and is essential for meiotic double strand break repair.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDaniel Wells,Emmanuelle Bitoun,Daniela Moralli,Gang Zhang,Anjali Hinch,Julia Jankowska,Peter Donnelly,Catherine Green,Simon R MyersPubMed 32744506
- Molecular cell 79:689-701.e102020The Configuration of RPA, RAD51, and DMC1 Binding in Meiosis Reveals the Nature of Critical Recombination Intermediates.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAnjali Gupta Hinch et. al.PubMed 32610038
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7 - 8
Preservative: 0.1% Sodium azide
Constituents: 1.815% Tris, 1.764% Sodium citrate, 0.021% PBS- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human RPA2 aa 200 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link P15927
Reactivity data
Select an application| IP | WB | IHC-P | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
| Mouse | Expected | Tested | Expected |
| Bat | Predicted | Predicted | Predicted |
| Chimpanzee | Predicted | Predicted | Predicted |
| Elephant | Predicted | Predicted | Predicted |
| Gorilla | Predicted | Predicted | Predicted |
| Horse | Predicted | Predicted | Predicted |
| Pig | Predicted | Predicted | Predicted |
| Rabbit | Predicted | Predicted | Predicted |
| Rhesus monkey | Predicted | Predicted | Predicted |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rabbit, Horse, Pig, Chimpanzee, Rhesus monkey, Gorilla, Bat, Elephant | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500.00000 - 1/2500.00000 | Notes - |
Species Mouse | Dilution info 1/500.00000 - 1/2500.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rabbit, Horse, Pig, Chimpanzee, Rhesus monkey, Gorilla, Bat, Elephant | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rabbit, Horse, Pig, Chimpanzee, Rhesus monkey, Gorilla, Bat, Elephant | Dilution info - | Notes - |
Associated Products
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Target data
Function
As part of the heterotrimeric replication protein A complex (RPA/RP-A), binds and stabilizes single-stranded DNA intermediates that form during DNA replication or upon DNA stress. It prevents their reannealing and in parallel, recruits and activates different proteins and complexes involved in DNA metabolism. Thereby, it plays an essential role both in DNA replication and the cellular response to DNA damage. In the cellular response to DNA damage, the RPA complex controls DNA repair and DNA damage checkpoint activation. Through recruitment of ATRIP activates the ATR kinase a master regulator of the DNA damage response. It is required for the recruitment of the DNA double-strand break repair factors RAD51 and RAD52 to chromatin in response to DNA damage. Also recruits to sites of DNA damage proteins like XPA and XPG that are involved in nucleotide excision repair and is required for this mechanism of DNA repair. Also plays a role in base excision repair (BER) probably through interaction with UNG. Also recruits SMARCAL1/HARP, which is involved in replication fork restart, to sites of DNA damage. May also play a role in telomere maintenance. RPA stimulates 5'-3' helicase activity of BRIP1/FANCJ (PubMed:17596542).
Alternative names
REPA2, RPA32, RPA34, RPA2, Replication protein A 32 kDa subunit, RP-A p32, Replication factor A protein 2, Replication protein A 34 kDa subunit, RF-A protein 2, RP-A p34
Recommended products
Anti-RPA32/RPA2 antibody (ab10359) is a rabbit polyclonal antibody that is used to detect RPA32/RPA2 in Western Blot, IP, IHC-P. Suitable for Human, Mouse samples.
- Over 10 publications
- Trusted since 2004
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7 - 8
Preservative: 0.1% Sodium azide
Constituents: 1.815% Tris, 1.764% Sodium citrate, 0.021% PBS- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human RPA2 aa 200 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link P15927
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
Immunoprecipitation - Anti-RPA32/RPA2 antibody (ab10359)
HeLa whole cell lysate cells treated with 100 μM etoposide for 4 hours (+) or mock treated (-).
Affinity purified rabbit anti-RPA32 antibody (3 μg) and rabbit anti-Phospho RPA32 antibody (1/100 dilution) were used.
Detection: Chemiluminescence
All lanes: Immunoprecipitation - Anti-RPA32/RPA2 antibody (ab10359)
Predicted band size: 29 kDa
Exposure time: 3min
Western blot - Anti-RPA32/RPA2 antibody (ab10359)
All lanes: Western blot - Anti-RPA32/RPA2 antibody (ab10359) at 0.1 µg/mL
Lane 1: HeLa whole cell lysate at 5 µg
Lane 2: HEK293T whole cell lysate at 5 µg
Lane 3: Jurkat whole cell lysate at 5 µg
Lane 4: Mouse TCMK-1 whole cell lysate at 5 µg
Lane 5: Mouse NIH 3T3 whole cell lysate at 5 µg
Predicted band size: 29 kDa
Exposure time: 10s
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPA32/RPA2 antibody (ab10359)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling RPA32/RPA2 with ab10359 at 1/5000 (0.2μg/ml). Detection: DAB.
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