AB10359

Anti-RPA32/RPA2 antibody

Name: Anti-RPA32/RPA2 antibody (ab10359) | Abcam
Brand: Abcam Limited
SKU: AB10359
Price: 420 USD
Availability: InStock

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(24 Publications)

Anti-RPA32/RPA2 antibody (ab10359) is a rabbit polyclonal antibody detecting RPA32/RPA2 in Western Blot, IP, IHC-P. Suitable for Human, Mouse.

- Over 10 publications
- Trusted since 2004

View Alternative Names

REPA2, RPA32, RPA34, RPA2, Replication protein A 32 kDa subunit, RP-A p32, Replication factor A protein 2, Replication protein A 34 kDa subunit, RF-A protein 2, RP-A p34

3 Images

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Immunoprecipitation - Anti-RPA32/RPA2 antibody (AB10359)

HeLa whole cell lysate cells treated with 100 μM etoposide for 4 hours (+) or mock treated (-).

Affinity purified rabbit anti-RPA32 antibody (3 μg) and rabbit anti-Phospho RPA32 antibody (1/100 dilution) were used.

Detection : Chemiluminescence

All lanes:

Immunoprecipitation - Anti-RPA32/RPA2 antibody (ab10359)

Predicted band size: 29 kDa

false

Exposure time: 3min

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPA32/RPA2 antibody (AB10359)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPA32/RPA2 antibody (AB10359)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling RPA32/RPA2 with ab10359 at 1/5000 (0.2μg/ml). Detection : DAB.

Unknown

Western blot - Anti-RPA32/RPA2 antibody (AB10359)

All lanes:

Western blot - Anti-RPA32/RPA2 antibody (ab10359) at 0.1 µg/mL

Lane 1:

HeLa whole cell lysate at 5 µg

Lane 2:

HEK293T whole cell lysate at 5 µg

Lane 3:

Jurkat whole cell lysate at 5 µg

Lane 4:

Mouse TCMK-1 whole cell lysate at 5 µg

Lane 5:

Mouse NIH 3T3 whole cell lysate at 5 µg

Predicted band size: 29 kDa

false

Exposure time: 10s

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

IP, IHC-P, WB

applications

Immunogen

Synthetic Peptide within Human RPA2 aa 200 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P15927

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Reactivity data

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Product details

What is this antibody validated in?
Anti-RPA32/RPA2 antibody (ab10359) is a rabbit polyclonal antibody and is validated for use in Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC-P) in Human, Mouse samples.

What is the molecular weight of RPA32/RPA2?
Anti-RPA32/RPA2 (ab10359) specifically detects a band for RPA32/RPA2 (UniProt: P15927) at a molecular weight of 32kDa.

Trusted by the scientific community
Anti-RPA32/RPA2 (ab10359) was first used in a scientific publication in 2004 and has been cited over 10 times in peer-reviewed journals.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 - 8 Preservative: 0.1% Sodium azide Constituents: PBS, 1.815% Tris, 1.764% Sodium citrate

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

As part of the heterotrimeric replication protein A complex (RPA/RP-A), binds and stabilizes single-stranded DNA intermediates that form during DNA replication or upon DNA stress. It prevents their reannealing and in parallel, recruits and activates different proteins and complexes involved in DNA metabolism. Thereby, it plays an essential role both in DNA replication and the cellular response to DNA damage. In the cellular response to DNA damage, the RPA complex controls DNA repair and DNA damage checkpoint activation. Through recruitment of ATRIP activates the ATR kinase a master regulator of the DNA damage response. It is required for the recruitment of the DNA double-strand break repair factors RAD51 and RAD52 to chromatin in response to DNA damage. Also recruits to sites of DNA damage proteins like XPA and XPG that are involved in nucleotide excision repair and is required for this mechanism of DNA repair. Also plays a role in base excision repair (BER) probably through interaction with UNG. Also recruits SMARCAL1/HARP, which is involved in replication fork restart, to sites of DNA damage. May also play a role in telomere maintenance. RPA stimulates 5'-3' helicase activity of BRIP1/FANCJ (PubMed : 17596542).

See full target information RPA2

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Publications (24)

Recent publications for all applications. Explore the full list and refine your search

PLoS genetics 21:e1011772 PubMed40758762

2025

Recombination plasticity in response to temperature variation in reptiles.

Applications

Unspecified application

Species

Unspecified reactive species

Laura González-Rodelas,Cristina Marín-García,Clara Romero,Gala Pujol,Laia Marín-Gual,Lukáš Kratochvíl,Aurora Ruiz-Herrera

Nature 640:1093-1102 PubMed40205037

2025

Comprehensive interrogation of synthetic lethality in the DNA damage response.

Applications

Unspecified application

Species

Unspecified reactive species

John Fielden,Sebastian M Siegner,Danielle N Gallagher,Markus S Schröder,Maria Rosaria Dello Stritto,Simon Lam,Lena Kobel,Moritz F Schlapansky,Stephen P Jackson,Petr Cejka,Marco Jost,Jacob E Corn

Animals : an open access journal from MDPI 14: PubMed39595299

2024

The Germline-Restricted Chromosome of Male Zebra Finches in Meiotic Prophase I: A Proteinaceous Scaffold and Chromatin Modifications.

Applications

Unspecified application

Species

Unspecified reactive species

Sergey Matveevsky

Nucleic acids research 52:9463-9480 PubMed38989615

2024

BOD1L mediates chromatin binding and non-canonical function of H3K4 methyltransferase SETD1A.

Applications

Unspecified application

Species

Unspecified reactive species

Takayuki Hoshii,Sota Kikuchi,Tomoya Kujirai,Takeshi Masuda,Tomoko Ito,Satoshi Yasuda,Makoto Matsumoto,Bahityar Rahmutulla,Masaki Fukuyo,Takeshi Murata,Hitoshi Kurumizaka,Atsushi Kaneda

Science (New York, N.Y.) 385:eado3867 PubMed38900911

2024

Structure and repair of replication-coupled DNA breaks.

Applications

Unspecified application

Species

Unspecified reactive species

Raphael Pavani,Veenu Tripathi,Kyle B Vrtis,Dali Zong,Raj Chari,Elsa Callen,Ajith V Pankajam,Gang Zhen,Gabriel Matos-Rodrigues,Jiajie Yang,Shuheng Wu,Giordano Reginato,Wei Wu,Petr Cejka,Johannes C Walter,André Nussenzweig

Genome research 33:2018-2027 PubMed37977820

2023

Characterization of meiotic recombination intermediates through gene knockouts in founder hybrid mice.

Applications

Unspecified application

Species

Unspecified reactive species

Benjamin Davies,Gang Zhang,Daniela Moralli,Samy Alghadban,Daniel Biggs,Chris Preece,Peter Donnelly,Anjali Gupta Hinch

Frontiers in cell and developmental biology 11:1147610 PubMed37181752

2023

Divergent patterns of meiotic double strand breaks and synapsis initiation dynamics suggest an evolutionary shift in the meiosis program between American and Australian marsupials.

Applications

Unspecified application

Species

Unspecified reactive species

F Javier Valero-Regalón,Mireia Solé,Pablo López-Jiménez,María Valerio-de Arana,Marta Martín-Ruiz,Roberto de la Fuente,Laia Marín-Gual,Marilyn B Renfree,Geoff Shaw,Soledad Berríos,Raúl Fernández-Donoso,Paul D Waters,Aurora Ruiz-Herrera,Rocío Gómez,Jesús Page

Cell reports 42:112062 PubMed36729836

2023

GEN1 promotes common fragile site expression.

Applications

Unspecified application

Species

Unspecified reactive species

Anaid Benitez,Marie Sebald,Radhakrishnan Kanagaraj,Monica C Rodrigo-Brenni,Ying Wai Chan,Chih-Chao Liang,Stephen C West

eLife 11: PubMed35575473

2022

DNA-PK promotes DNA end resection at DNA double strand breaks in G cells.

Applications

Unspecified application

Species

Unspecified reactive species

Faith C Fowler,Bo-Ruei Chen,Nicholas Zolnerowich,Wei Wu,Raphael Pavani,Jacob Paiano,Chelsea Peart,Zulong Chen,André Nussenzweig,Barry P Sleckman,Jessica K Tyler

Genes & development 35:1356-1367 PubMed34503990

2021

Role of 53BP1 in end protection and DNA synthesis at DNA breaks.

Applications

Unspecified application

Species

Unspecified reactive species

Jacob Paiano,Nicholas Zolnerowich,Wei Wu,Raphael Pavani,Chen Wang,Hongzhi Li,Li Zheng,Binghui Shen,Barry P Sleckman,Bo-Ruei Chen,André Nussenzweig

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-RPA32/RPA2 antibody

Immunoprecipitation - Anti-RPA32/RPA2 antibody (AB10359)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPA32/RPA2 antibody (AB10359)

Western blot - Anti-RPA32/RPA2 antibody (AB10359)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (24)

Product promise