AB240637

Anti-RPA32/RPA2 antibody [EPR22950-81]

BOND RX™ Validated
RabMAb
Recombinant
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(1 Publication)

Rabbit Recombinant Monoclonal RPA32/RPA2 antibody. Suitable for IP, WB, ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Human samples. Cited in 1 publication.

View Alternative Names

REPA2, RPA32, RPA34, RPA2, Replication protein A 32 kDa subunit, RP-A p32, Replication factor A protein 2, Replication protein A 34 kDa subunit, RF-A protein 2, RP-A p34

12 Images

Flow Cytometry (Intracellular) - Anti-RPA32/RPA2 antibody [EPR22950-81] (AB240637)

Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-RPA32/RPA2 antibody [EPR22950-81] (AB240637)

Intracellular flow cytometric analysis of 4% paraformaldehyde fixed. 90% methanol permeabilized HeLa (human cervix adenocarcinoma epithelial cell) cells labeling RPA32/RPA2 with ab240637 at 1/600 dilution (Red) compared with a Rabbit monoclonal IgG (ab172730) isotype control (black)and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPA32/RPA2 antibody [EPR22950-81] (AB240637)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPA32/RPA2 antibody [EPR22950-81] (AB240637)

Immunohistochemical analysis of paraffin-embedded human glioma tissue labeling RPA32/RPA2 with ab240637 at 1/5000 dilution (0.103 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Nuclear staining on human glioma (PMID : 20154705) is observed. The section was incubated with ab240637 for 10 mins at RT. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Immunocytochemistry/ Immunofluorescence - Anti-RPA32/RPA2 antibody [EPR22950-81] (AB240637)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-RPA32/RPA2 antibody [EPR22950-81] (AB240637)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (human cervix adenocarcinoma epithelial cell) cells labeling RPA32/RPA2 with ab240637 at 1/100 dilution, followed by anti-RPA32/RPA2 ab150077 AlexaFluor^®488 Goat anti-Rabbit secondary antibody at 1/1000 dilution (Green). Confocal image showing nuclear staining in HeLa cell line is observed. ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) was used to counterstain tubulin at 1/200 dilution (Red). The nuclear counterstain was DAPI (Blue).

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is AlexaFluor^®488 Goat anti-Rabbit secondary (ab150077) at 1/1000 dilution.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPA32/RPA2 antibody [EPR22950-81] (AB240637)

Immunohistochemical analysis of paraffin-embedded human colon tissue labeling RPA32/RPA2 with ab240637 at 1/5000 dilution (0.103 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Nuclear staining on human colon (PMID : 20154705) is observed. The section was incubated with ab240637 for 10 mins at RT. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Unknown

Immunoprecipitation - Anti-RPA32/RPA2 antibody [EPR22950-81] (AB240637)

RPA32/RPA2 was immunoprecipitated from 0.35 mg HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate with ab240637 at 1/30 dilution (2μg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab240637 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1/5000 dilution.

Lane 1 : HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate 10μg.

Lane 2 : ab240637 IP in HeLa whole cell lysate.

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab240637 in HeLa whole cell lysate.

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 8 seconds.

All lanes:

Immunoprecipitation - Anti-RPA32/RPA2 antibody [EPR22950-81] (ab240637)

Predicted band size: 29 kDa

Observed band size: 32 kDa

false

Lab

Western blot - Anti-RPA32/RPA2 antibody [EPR22950-81] (AB240637)

Exposure time : 5.5 seconds.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

All lanes:

Western blot - Anti-RPA32/RPA2 antibody [EPR22950-81] (ab240637) at 1/1000 dilution

All lanes:

Human brain tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 29 kDa

Observed band size: 32 kDa

false

Lab

Western blot - Anti-RPA32/RPA2 antibody [EPR22950-81] (AB240637)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

The molecular weight of RPA32/RPA2 is upshifted in response to camptothecin treatment (PMID : 17928296).

To minimize protein degradation, cells were lysed immediately after harvest and then applied to a gel and transfer membrane for Western blotting as soon as possible.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) (1 : 200000) (36KDa).

All lanes:

Western blot - Anti-RPA32/RPA2 (phospho S33) antibody [EPR29612-551] (<a href='/en-us/products/primary-antibodies/rpa32-rpa2-phospho-s33-antibody-epr29612-551-ab325750'>ab325750</a>) at 1/1000 dilution

Lane 1:

Untreated 293T (human embryonic kidney epithelial cell) fresh whole cell lysate (untreated membrane) at 50 µg

Lane 2:

293T treated with 1 μM camptothecin for 3 h fresh whole cell lysate (untreated membrane) at 50 µg

Lane 3:

Untreated 293T (human embryonic kidney epithelial cell) fresh whole cell lysate (alkaline phosphatase treated membrane) at 50 µg

Lane 4:

293T treated with 1 μM camptothecin for 3 h fresh whole cell lysate (alkaline phosphatase treated membrane) at 50 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 32 kDa,36 kDa,30 kDa

false

Exposure time: 180s

Lab

Western blot - Anti-RPA32/RPA2 antibody [EPR22950-81] (AB240637)

Exposure time : 5.5 seconds.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

All lanes:

Western blot - Anti-RPA32/RPA2 antibody [EPR22950-81] (ab240637) at 1/1000 dilution

Lane 1:

HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate at 20 µg

Lane 2:

HUVEC (human umbilical vein endothelial cell), whole cell lysate at 20 µg

Lane 3:

U-2 OS (human bone osteosarcoma epithelial cell), whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 29 kDa

Observed band size: 32 kDa

false

Supplier Data

Western blot - Anti-RPA32/RPA2 antibody [EPR22950-81] (AB240637)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

The molecular weight of RPA32/RPA2 is upshifted in response to Etoposide treatment (PMID : 21731742).

To minimize protein degradation, cells were lysed immediately after harvest and then applied to a gel and transfer membrane for Western blotting as soon as possible.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

In Western blot, Anti-RPA32/RPA2 antibody (ab240637) staining at 1/1000 dilution.

All lanes:

Western blot - Anti-RPA32/RPA2 (phospho S4) antibody [EPR29611-59] (<a href='/en-us/products/primary-antibodies/rpa32-rpa2-phospho-s4-antibody-epr29611-59-ab325317'>ab325317</a>) at 1/1000 dilution

Lane 1:

Untreated HeLa (human cervical adenocarcinoma epithelial cell) fresh whole cell lysate (untreated membrane) at 50 µg

Lane 2:

HeLa treated with 100µM Etoposide for 4h fresh whole cell lysate (untreated membrane) at 50 µg

Lane 3:

Untreated HeLa fresh whole cell lysate (alkaline phosphatase treated membrane) at 50 µg

Lane 4:

HeLa treated with 100µM Etoposide for 4h fresh whole cell lysate (alkaline phosphatase treated membrane) at 50 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 32 kDa,30 kDa,36 kDa

false

Exposure time: 26s

Supplier Data

Western blot - Anti-RPA32/RPA2 antibody [EPR22950-81] (AB240637)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

The molecular weight of RPA32/RPA2 is upshifted in response to camptothecin treatment (PMID : 21731742).

To minimize protein degradation, cells were lysed immediately after harvest and then applied to a gel and transfer membrane for Western blotting as soon as possible.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

In Western blot, Anti-RPA32/RPA2 antibody (ab240637) staining at 1/1000 dilution.

All lanes:

Western blot - Anti-RPA32/RPA2 (phospho S4) antibody [EPR29611-59] (<a href='/en-us/products/primary-antibodies/rpa32-rpa2-phospho-s4-antibody-epr29611-59-ab325317'>ab325317</a>) at 1/1000 dilution

Lane 1:

Untreated 293T (human embryonic kidney epithelial cell) fresh whole cell lysate (untreated membrane) at 50 µg

Lane 2:

293T treated with 1µM camptothecin for 3h fresh whole cell lysate (untreated membrane) at 50 µg

Lane 3:

Untreated 293T (human embryonic kidney epithelial cell) fresh whole cell lysate (alkaline phosphatase treated membrane) at 50 µg

Lane 4:

293T treated with 1µM camptothecin for 3h fresh whole cell lysate (alkaline phosphatase treated membrane) at 50 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 32 kDa,30 kDa,36 kDa

false

Exposure time: 15s

Lab

Western blot - Anti-RPA32/RPA2 antibody [EPR22950-81] (AB240637)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

The molecular weight of RPA32/RPA2 is upshifted in response to Etoposide treatment (PMID : 17928296).

To minimize protein degradation, cells were lysed immediately after harvest and then applied to a gel and transfer membrane for Western blotting as soon as possible.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) (1 : 200000) (36KDa).

All lanes:

Lane 1:

Untreated HeLa (human cervical adenocarcinoma epithelial cell) fresh whole cell lysate at 50 µg

Lane 2:

HeLa treated with 100 μM Etoposide for 16 hours fresh whole cell lysate at 50 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 32 kDa,36 kDa,30 kDa

false

Exposure time: 180s

Supplier Data

Western blot - Anti-RPA32/RPA2 antibody [EPR22950-81] (AB240637)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

The molecular weight of RPA32/RPA2 is upshifted in response to camptothecin treatment (PMID : 21731742).

To minimize protein degradation, cells were lysed immediately after harvest and then applied to a gel and transfer membrane for Western blotting as soon as possible.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

In Western blot, Anti-RPA32/RPA2 antibody (ab240637) staining at 1/1000 dilution.

All lanes:

Western blot - Anti-RPA32/RPA2 (phospho S4) antibody [EPR29611-59] (<a href='/en-us/products/primary-antibodies/rpa32-rpa2-phospho-s4-antibody-epr29611-59-ab325317'>ab325317</a>) at 1/1000 dilution

Lane 1:

Untreated NIH/3T3 (mouse embryonic fibroblast) fresh whole cell lysate (untreated membrane) at 50 µg

Lane 2:

NIH/3T3 treated with 1µM camptothecin for 3h fresh whole cell lysate (untreated membrane) at 50 µg

Lane 3:

Untreated NIH/3T3 fresh whole cell lysate (alkaline phosphatase treated membrane) at 50 µg

Lane 4:

NIH/3T3 treated with 1µM camptothecin for 3h fresh whole cell lysate (alkaline phosphatase treated membrane) at 50 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 32 kDa,30 kDa,36 kDa

false

Exposure time: 26s

Carrier free

Anti-RPA32/RPA2 antibody [EPR22950-81] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR22950-81

Isotype

IgG

Carrier free

Reacts with

Human

Applications

Flow Cyt (Intra), IP, IHC-P, WB, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "ChIP" : {"fullname" : "ChIP", "shortname":"ChIP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/30", "IP-species-notes": "", "ChIP-species-checked": "notRecommended", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/600", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/5000", "IHCP-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

As part of the heterotrimeric replication protein A complex (RPA/RP-A), binds and stabilizes single-stranded DNA intermediates that form during DNA replication or upon DNA stress. It prevents their reannealing and in parallel, recruits and activates different proteins and complexes involved in DNA metabolism. Thereby, it plays an essential role both in DNA replication and the cellular response to DNA damage. In the cellular response to DNA damage, the RPA complex controls DNA repair and DNA damage checkpoint activation. Through recruitment of ATRIP activates the ATR kinase a master regulator of the DNA damage response. It is required for the recruitment of the DNA double-strand break repair factors RAD51 and RAD52 to chromatin in response to DNA damage. Also recruits to sites of DNA damage proteins like XPA and XPG that are involved in nucleotide excision repair and is required for this mechanism of DNA repair. Also plays a role in base excision repair (BER) probably through interaction with UNG. Also recruits SMARCAL1/HARP, which is involved in replication fork restart, to sites of DNA damage. May also play a role in telomere maintenance. RPA stimulates 5'-3' helicase activity of BRIP1/FANCJ (PubMed : 17596542).

See full target information RPA2

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 16:3312 PubMed40204713

2025

GRAMD1B is a regulator of lipid homeostasis, autophagic flux and phosphorylated tau.

Applications

Unspecified application

Species

Unspecified reactive species

Diana Acosta Ingram,Emir Turkes,Tae Yeon Kim,Sheeny Vo,Nicholas Sweeney,Marie-Amandine Bonte,Ryan Rutherford,Dominic L Julian,Meixia Pan,Jacob Marsh,Andrea R Argouarch,Min Wu,Douglas W Scharre,Erica H Bell,Lawrence S Honig,Jean Paul Vonsattel,Geidy E Serrano,Thomas G Beach,Celeste M Karch,Aimee W Kao,Mark E Hester,Xianlin Han,Hongjun Fu

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-RPA32/RPA2 antibody [EPR22950-81]

Flow Cytometry (Intracellular) - Anti-RPA32/RPA2 antibody [EPR22950-81] (AB240637)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPA32/RPA2 antibody [EPR22950-81] (AB240637)

Immunocytochemistry/ Immunofluorescence - Anti-RPA32/RPA2 antibody [EPR22950-81] (AB240637)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPA32/RPA2 antibody [EPR22950-81] (AB240637)

Immunoprecipitation - Anti-RPA32/RPA2 antibody [EPR22950-81] (AB240637)

Western blot - Anti-RPA32/RPA2 antibody [EPR22950-81] (AB240637)

Western blot - Anti-RPA32/RPA2 antibody [EPR22950-81] (AB240637)

Western blot - Anti-RPA32/RPA2 antibody [EPR22950-81] (AB240637)

Western blot - Anti-RPA32/RPA2 antibody [EPR22950-81] (AB240637)

Western blot - Anti-RPA32/RPA2 antibody [EPR22950-81] (AB240637)

Western blot - Anti-RPA32/RPA2 antibody [EPR22950-81] (AB240637)

Western blot - Anti-RPA32/RPA2 antibody [EPR22950-81] (AB240637)

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Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (1)

Product promise