Rabbit Recombinant Monoclonal RPA32/RPA2 antibody. Carrier free. Suitable for ChIC/CUT&RUN-seq, IHC-P, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human, Rat samples.
Images
![Immunocytochemistry/ Immunofluorescence - Anti-RPA32/RPA2 antibody [EPR2876(2)] - BSA and Azide free (AB247754), expandable thumbnail](https://content.abcam.com/products/images/rpa32-rpa2-antibody-epr28762-bsa-and-azide-free-ab247754--immunocytochemistry-immunofluorescence-img19371.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-RPA32/RPA2 antibody [EPR2876(2)] - BSA and Azide free (AB247754)")
![Immunocytochemistry/ Immunofluorescence - Anti-RPA32/RPA2 antibody [EPR2876(2)] - BSA and Azide free (AB247754), expandable thumbnail](https://content.abcam.com/products/images/rpa32-rpa2-antibody-epr28762-bsa-and-azide-free-ab247754--immunocytochemistry-immunofluorescence-img69045.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-RPA32/RPA2 antibody [EPR2876(2)] - BSA and Azide free (AB247754)")
![Flow Cytometry (Intracellular) - Anti-RPA32/RPA2 antibody [EPR2876(2)] - BSA and Azide free (AB247754), expandable thumbnail](https://content.abcam.com/products/images/rpa32-rpa2-antibody-epr28762-bsa-and-azide-free-ab247754--flow-cytometry-intracellular-img78315.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-RPA32/RPA2 antibody [EPR2876(2)] - BSA and Azide free (AB247754)")
![Flow Cytometry (Intracellular) - Anti-RPA32/RPA2 antibody [EPR2876(2)] - BSA and Azide free (AB247754), expandable thumbnail](https://content.abcam.com/products/images/rpa32-rpa2-antibody-epr28762-bsa-and-azide-free-ab247754--flow-cytometry-intracellular-img136357.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-RPA32/RPA2 antibody [EPR2876(2)] - BSA and Azide free (AB247754)")
![Western blot - Anti-RPA32/RPA2 antibody [EPR2876(2)] - BSA and Azide free (AB247754), expandable thumbnail](https://content.abcam.com/products/images/rpa32-rpa2-antibody-epr28762-bsa-and-azide-free-ab247754--western-blot-img111786.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-RPA32/RPA2 antibody [EPR2876(2)] - BSA and Azide free (AB247754)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| ChIC/CUT&RUN-seq | IHC-P | IP | WB | ICC/IF | Flow Cyt (Intra) | |
|---|---|---|---|---|---|---|
| Human | Tested | Tested | Not recommended | Tested | Tested | Tested |
| Rat | Expected | Expected | Not recommended | Expected | Tested | Tested |
ChIC/CUT&RUN-seq
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info Use at an assay dependent concentration. | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Antigen retrieval is recommended. Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info Use at an assay dependent concentration. | Notes - |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Human | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info Use at an assay dependent concentration. | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Human | Dilution info - | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Human | Dilution info - | Notes - |
Associated Products
Select an associated product type
2 products for Alternative Product
Target data
Function
As part of the heterotrimeric replication protein A complex (RPA/RP-A), binds and stabilizes single-stranded DNA intermediates that form during DNA replication or upon DNA stress. It prevents their reannealing and in parallel, recruits and activates different proteins and complexes involved in DNA metabolism. Thereby, it plays an essential role both in DNA replication and the cellular response to DNA damage. In the cellular response to DNA damage, the RPA complex controls DNA repair and DNA damage checkpoint activation. Through recruitment of ATRIP activates the ATR kinase a master regulator of the DNA damage response. It is required for the recruitment of the DNA double-strand break repair factors RAD51 and RAD52 to chromatin in response to DNA damage. Also recruits to sites of DNA damage proteins like XPA and XPG that are involved in nucleotide excision repair and is required for this mechanism of DNA repair. Also plays a role in base excision repair (BER) probably through interaction with UNG. Also recruits SMARCAL1/HARP, which is involved in replication fork restart, to sites of DNA damage. May also play a role in telomere maintenance. RPA stimulates 5'-3' helicase activity of BRIP1/FANCJ (PubMed:17596542).
Alternative names
REPA2, RPA32, RPA34, RPA2, Replication protein A 32 kDa subunit, RP-A p32, Replication factor A protein 2, Replication protein A 34 kDa subunit, RF-A protein 2, RP-A p34
Recommended products
Rabbit Recombinant Monoclonal RPA32/RPA2 antibody. Carrier free. Suitable for ChIC/CUT&RUN-seq, IHC-P, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human, Rat samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR2876(2)
- Purification technique
- Affinity purification
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab247754 is the carrier-free version of Anti-RPA32/RPA2 antibody [EPR2876(2)] ab109084.
Species reactivity
Mouse: We have preliminary internal testing data to indicate this antibody may not react with this species.
Please contact us for more information.
Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
8 product images
![Immunocytochemistry/ Immunofluorescence - Anti-RPA32/RPA2 antibody [EPR2876(2)] - BSA and Azide free (ab247754), expandable thumbnail](https://content.abcam.com/products/images/rpa32-rpa2-antibody-epr28762-bsa-and-azide-free-ab247754--immunocytochemistry-immunofluorescence-img19371.jpg "Immunocytochemistry/ Immunofluorescence - Anti-RPA32/RPA2 antibody [EPR2876(2)] - BSA and Azide free (ab247754)")
Immunocytochemistry/ Immunofluorescence - Anti-RPA32/RPA2 antibody [EPR2876(2)] - BSA and Azide free (ab247754)
This data was developed using Anti-RPA32/RPA2 antibody [EPR2876(2)] ab109084, the same antibody clone in a different buffer formulation.
Immunocytochemistry/ Immunofluorescence analysis of C6 (rat glial tumor glial cell) cells labeling RPA32/RPA2 with purified Anti-RPA32/RPA2 antibody [EPR2876(2)] ab109084 at 1:50 (9.34 μg/ml). Cells were fixed in 100% Methanol and permeabilized with None. Cells were counterstained with Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 μg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) was used as the secondary antibody at 1:1000 (2 μg/ml) dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
![Immunocytochemistry/ Immunofluorescence - Anti-RPA32/RPA2 antibody [EPR2876(2)] - BSA and Azide free (ab247754), expandable thumbnail](https://content.abcam.com/products/images/rpa32-rpa2-antibody-epr28762-bsa-and-azide-free-ab247754--immunocytochemistry-immunofluorescence-img69045.jpg "Immunocytochemistry/ Immunofluorescence - Anti-RPA32/RPA2 antibody [EPR2876(2)] - BSA and Azide free (ab247754)")
Immunocytochemistry/ Immunofluorescence - Anti-RPA32/RPA2 antibody [EPR2876(2)] - BSA and Azide free (ab247754)
This data was developed using Anti-RPA32/RPA2 antibody [EPR2876(2)] ab109084, the same antibody clone in a different buffer formulation.
Immunocytochemistry/ Immunofluorescence analysis of HeLa (human cervix adenocarcinoma epithelial cell) cells labeling RPA32/RPA2 with purified Anti-RPA32/RPA2 antibody [EPR2876(2)] ab109084 at 1:50 (9.34 μg/ml). Cells were fixed in 100% Methanol and permeabilized with None. Cells were counterstained with Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 μg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) was used as the secondary antibody at 1:1000 (2 μg/ml) dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
![Flow Cytometry (Intracellular) - Anti-RPA32/RPA2 antibody [EPR2876(2)] - BSA and Azide free (ab247754), expandable thumbnail](https://content.abcam.com/products/images/rpa32-rpa2-antibody-epr28762-bsa-and-azide-free-ab247754--flow-cytometry-intracellular-img78315.jpg "Flow Cytometry (Intracellular) - Anti-RPA32/RPA2 antibody [EPR2876(2)] - BSA and Azide free (ab247754)")
Flow Cytometry (Intracellular) - Anti-RPA32/RPA2 antibody [EPR2876(2)] - BSA and Azide free (ab247754)
This data was developed using Anti-RPA32/RPA2 antibody [EPR2876(2)] ab109084, the same antibody clone in a different buffer formulation.
Intracellular Flow Cytometry analysis of C6 (rat glial tumor glial cell) cells labeling RPA32/RPA2 with purified Anti-RPA32/RPA2 antibody [EPR2876(2)] ab109084 at 1/460 dilution (1.01 μg/ml) - Red. Cells were fixed with 4% paraformaldehyde . A Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody was used at 1/2000 dilution. Isotype control - Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) - Black. Unlabeled control - Blue. Untreated cells - Green
![Flow Cytometry (Intracellular) - Anti-RPA32/RPA2 antibody [EPR2876(2)] - BSA and Azide free (ab247754), expandable thumbnail](https://content.abcam.com/products/images/rpa32-rpa2-antibody-epr28762-bsa-and-azide-free-ab247754--flow-cytometry-intracellular-img136357.jpg "Flow Cytometry (Intracellular) - Anti-RPA32/RPA2 antibody [EPR2876(2)] - BSA and Azide free (ab247754)")
Flow Cytometry (Intracellular) - Anti-RPA32/RPA2 antibody [EPR2876(2)] - BSA and Azide free (ab247754)
This data was developed using Anti-RPA32/RPA2 antibody [EPR2876(2)] ab109084, the same antibody clone in a different buffer formulation.
Intracellular Flow Cytometry analysis of HeLa (human cervix adenocarcinoma epithelial cell) cells labeling RPA32/RPA2 with purified Anti-RPA32/RPA2 antibody [EPR2876(2)] ab109084 at 1/460 dilution (1.01 μg/ml) - Red. Cells were fixed with 4% paraformaldehyde . A Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody was used at 1/2000 dilution. Isotype control - Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) - Black. Unlabeled control - Blue. Untreated cells - Green
![Western blot - Anti-RPA32/RPA2 antibody [EPR2876(2)] - BSA and Azide free (ab247754), expandable thumbnail](https://content.abcam.com/products/images/rpa32-rpa2-antibody-epr28762-bsa-and-azide-free-ab247754--western-blot-img111786.jpg "Western blot - Anti-RPA32/RPA2 antibody [EPR2876(2)] - BSA and Azide free (ab247754)")
Western blot - Anti-RPA32/RPA2 antibody [EPR2876(2)] - BSA and Azide free (ab247754)
This data was developed using Anti-RPA32/RPA2 antibody [EPR2876(2)] ab109084, the same antibody clone in a different buffer formulation.
All lanes: Western blot - Anti-RPA32/RPA2 antibody [EPR2876(2)] (Anti-RPA32/RPA2 antibody [EPR2876(2)] ab109084) at 1/1000 dilution
Lane 1: HeLa cell lysate at 10 µg
Lane 2: HUVEC cell lysate at 10 µg
Lane 3: HepG2 cell lysate at 10 µg
Lane 4: MCF7 cell lysate at 10 µg
Predicted band size: 29 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPA32/RPA2 antibody [EPR2876(2)] - BSA and Azide free (ab247754), expandable thumbnail](https://content.abcam.com/products/images/rpa32-rpa2-antibody-epr28762-bsa-and-azide-free-ab247754--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img137819.gif "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPA32/RPA2 antibody [EPR2876(2)] - BSA and Azide free (ab247754)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPA32/RPA2 antibody [EPR2876(2)] - BSA and Azide free (ab247754)
This data was developed using Anti-RPA32/RPA2 antibody [EPR2876(2)] ab109084, the same antibody clone in a different buffer formulation.Anti-RPA32/RPA2 antibody [EPR2876(2)] ab109084 at 1/250 dilution staining RPA32/RPA2 in Human colon by Immunohistochemistry, Paraffin-embedded tissue. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPA32/RPA2 antibody [EPR2876(2)] - BSA and Azide free (ab247754), expandable thumbnail](https://content.abcam.com/products/images/rpa32-rpa2-antibody-epr28762-bsa-and-azide-free-ab247754--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img137190.gif "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPA32/RPA2 antibody [EPR2876(2)] - BSA and Azide free (ab247754)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPA32/RPA2 antibody [EPR2876(2)] - BSA and Azide free (ab247754)
This data was developed using Anti-RPA32/RPA2 antibody [EPR2876(2)] ab109084, the same antibody clone in a different buffer formulation.Anti-RPA32/RPA2 antibody [EPR2876(2)] ab109084 at 1/250 dilution staining RPA32/RPA2 in Human ovary carcinoma by Immunohistochemistry, Paraffin-embedded tissue. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
![ChIC/CUT&RUN sequencing - Anti-RPA32/RPA2 antibody [EPR2876(2)] - BSA and Azide free (ab247754), expandable thumbnail](https://content.abcam.com/products/images/rpa32-rpa2-antibody-epr28762-bsa-and-azide-free-ab247754--chic-cut-run-sequencing-img421717.jpg "ChIC/CUT&RUN sequencing - Anti-RPA32/RPA2 antibody [EPR2876(2)] - BSA and Azide free (ab247754)")
ChIC/CUT&RUN sequencing - Anti-RPA32/RPA2 antibody [EPR2876(2)] - BSA and Azide free (ab247754)
ChIC/CUT&RUN was performed using a pAG-MNAse at a final concentration of 700 ng/mL, 2.5 x 10^5 HeLa (Human cervix adenocarcinoma epithelial cell line) cells and 5µg of Anti-RPA32/RPA2 antibody [EPR2876(2)] ab109084 [EPR2876(2)]. The resulting DNA was sequenced on the Illumina NovaSeq 6000 to a depth of 10 million reads. The negative IgG control Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730 is also shown.
Additional screenshots of mapped reads can be found in the Protocol booklet in the Support and downloads section.
The University of Geneva owns patents relevant to ChIC (Chromatin Immuno-Cleavage) methods.
This data was developed using Anti-RPA32/RPA2 antibody [EPR2876(2)] ab109084, the same antibody clone in a different buffer formulation.
![ChIC/CUT&RUN sequencing - Anti-RPA32/RPA2 antibody [EPR2876(2)] - BSA and Azide free (ab247754), expandable thumbnail](https://content.abcam.com/products/images/rpa32-rpa2-antibody-epr28762-bsa-and-azide-free-ab247754--chic-cut-run-sequencing-img421717.jpg/_jcr_content/renditions/webp-475.webp "ChIC/CUT&RUN sequencing - Anti-RPA32/RPA2 antibody [EPR2876(2)] - BSA and Azide free (ab247754)")
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