Rabbit Polyclonal RPA32/RPA2 phospho S33 antibody. Suitable for WB and reacts with Human samples. Cited in 7 publications. Immunogen corresponding to Synthetic Peptide within Human RPA2 phospho S33.
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- Signal transduction and targeted therapy 7:3542022A small molecule inhibitor of the UBE2F-CRL5 axis induces apoptosis and radiosensitization in lung cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTiantian Xu,Qisheng Ma,Yanan Li,Qing Yu,Peichen Pan,Yawen Zheng,Zhijian Li,Xiufang Xiong,Tingjun Hou,Bin Yu,Hongmin Liu,Yi SunPubMed 36253371
- iScience 25:1045912022A small-molecule Skp1 inhibitor elicits cell death by p53-dependent mechanism.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMuzammal Hussain,Yongzhi Lu,Muqddas Tariq,Hao Jiang,Yahai Shu,Shuang Luo,Qiang Zhu,Jiancun Zhang,Jinsong LiuPubMed 35789855
- NAR cancer 4:zcac0122022High replication stress and limited Rad51-mediated DNA repair capacity, but not oxidative stress, underlie oligodendrocyte precursor cell radiosensitivity.Applications:Unspecified applicationReactive species:Unspecified reactive speciesN Daniel Berger,Peter M Brownlee,Myra J Chen,Hali Morrison,Katalin Osz,Nicolas P Ploquin,Jennifer A Chan,Aaron A GoodarziPubMed 35425901
- PLoS biology 18:e30009912020Phosphorylation of seryl-tRNA synthetase by ATM/ATR is essential for hypoxia-induced angiogenesis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYi Shi et. al.PubMed 33351793
- Cell reports 32:1081662020BRD4 Prevents R-Loop Formation and Transcription-Replication Conflicts by Ensuring Efficient Transcription Elongation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDrake S Edwards,Rohin Maganti,Jarred P Tanksley,Jie Luo,James J H Park,Elena Balkanska-Sinclair,Jinjie Ling,Scott R FloydPubMed 32966794
- Genes 11:2020G-quadruplex Stabilization Fuels the ALT Pathway in ALT-positive Osteosarcoma Cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRoberta Amato et. al.PubMed 32183119
- Cancer research 78:5561-55732018Replication Protein A Availability during DNA Replication Stress Is a Major Determinant of Cisplatin Resistance in Ovarian Cancer Cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesFrançois Bélanger et. al.PubMed 30072396
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7 - 8
Preservative: 0.09% Sodium azide
Constituents: 99% Tris citrate/phosphate- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human RPA2 phospho S33. The exact immunogen used to generate this antibody is proprietary information. Database link P15927
Reactivity data
Select an application| WB | |
|---|---|
| Human | Tested |
| Mouse | Predicted |
| Chimpanzee | Predicted |
| Gorilla | Predicted |
| Orangutan | Predicted |
| Rhesus monkey | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/1000.00000 - 1/5000.00000 | Notes Numerous bands representing proteins that migrate more slowly than 60 to 70 kDa have been observed during WB. Under some conditions, at least two of these may be more intense than the band that represents phospho RPA32/RPA2 (S33). |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Chimpanzee, Rhesus monkey, Gorilla, Orangutan | Dilution info - | Notes - |
Associated Products
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Target data
Function
As part of the heterotrimeric replication protein A complex (RPA/RP-A), binds and stabilizes single-stranded DNA intermediates that form during DNA replication or upon DNA stress. It prevents their reannealing and in parallel, recruits and activates different proteins and complexes involved in DNA metabolism. Thereby, it plays an essential role both in DNA replication and the cellular response to DNA damage. In the cellular response to DNA damage, the RPA complex controls DNA repair and DNA damage checkpoint activation. Through recruitment of ATRIP activates the ATR kinase a master regulator of the DNA damage response. It is required for the recruitment of the DNA double-strand break repair factors RAD51 and RAD52 to chromatin in response to DNA damage. Also recruits to sites of DNA damage proteins like XPA and XPG that are involved in nucleotide excision repair and is required for this mechanism of DNA repair. Also plays a role in base excision repair (BER) probably through interaction with UNG. Also recruits SMARCAL1/HARP, which is involved in replication fork restart, to sites of DNA damage. May also play a role in telomere maintenance. RPA stimulates 5'-3' helicase activity of BRIP1/FANCJ (PubMed:17596542).
Alternative names
REPA2, RPA32, RPA34, RPA2, Replication protein A 32 kDa subunit, RP-A p32, Replication factor A protein 2, Replication protein A 34 kDa subunit, RF-A protein 2, RP-A p34
Recommended products
Rabbit Polyclonal RPA32/RPA2 phospho S33 antibody. Suitable for WB and reacts with Human samples. Cited in 7 publications. Immunogen corresponding to Synthetic Peptide within Human RPA2 phospho S33.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7 - 8
Preservative: 0.09% Sodium azide
Constituents: 99% Tris citrate/phosphate- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human RPA2 phospho S33. The exact immunogen used to generate this antibody is proprietary information. Database link P15927
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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In the unlikely event of one of our products not working as expected, you are covered by our product promise.
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1 product image
Western blot - Anti-RPA32/RPA2 (phospho S33) antibody (ab211877)
Lower panel shows Western Blotting for total RPA32/RPA2 using an affinity purified rabbit anti-RPA32/RPA2 antibody at 0.1 μg/ml with an exposure time of 10 seconds.
All lanes: Western blot - Anti-RPA32/RPA2 (phospho S33) antibody (ab211877) at 0.4 µg/mL
Lane 1: HeLa whole cell lysate treated with 100 µM etoposide for 4 hours at 50 µg
Lane 2: HeLa whole cell lysate mock treated at 50 µg
Developed using the ECL technique.
Predicted band size: 29 kDa
Exposure time: 10s
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