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AB211877

Anti-RPA32/RPA2 (phospho S33) antibody

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(15 Publications)

Rabbit Polyclonal RPA32/RPA2 phospho S33 antibody. Suitable for WB and reacts with Human samples. Cited in 15 publications. Immunogen corresponding to Synthetic Peptide within Human RPA2 phospho S33.

View Alternative Names

REPA2, RPA32, RPA34, RPA2, Replication protein A 32 kDa subunit, RP-A p32, Replication factor A protein 2, Replication protein A 34 kDa subunit, RF-A protein 2, RP-A p34

1 Images
Western blot - Anti-RPA32/RPA2 (phospho S33) antibody (AB211877)
  • WB

Supplier Data

Western blot - Anti-RPA32/RPA2 (phospho S33) antibody (AB211877)

Lower panel shows Western Blotting for total RPA32/RPA2 using an affinity purified rabbit anti-RPA32/RPA2 antibody at 0.1 μg/ml with an exposure time of 10 seconds.

All lanes:

Western blot - Anti-RPA32/RPA2 (phospho S33) antibody (ab211877) at 0.4 µg/mL

Lane 1:

HeLa whole cell lysate treated with 100 µM etoposide for 4 hours at 50 µg

Lane 2:

HeLa whole cell lysate mock treated at 50 µg

Predicted band size: 29 kDa

true

Exposure time: 10s

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB

applications

Immunogen

Synthetic Peptide within Human RPA2 phospho S33. The exact immunogen used to generate this antibody is proprietary information.

P15927

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "<p>Numerous bands representing proteins that migrate more slowly than 60 to 70 kDa have been observed during WB. Under some conditions, at least two of these may be more intense than the band that represents phospho RPA32/RPA2 (S33).</p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chimpanzee": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Gorilla": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rhesus monkey": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: 99% Tris citrate/phosphate
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Target data

As part of the heterotrimeric replication protein A complex (RPA/RP-A), binds and stabilizes single-stranded DNA intermediates that form during DNA replication or upon DNA stress. It prevents their reannealing and in parallel, recruits and activates different proteins and complexes involved in DNA metabolism. Thereby, it plays an essential role both in DNA replication and the cellular response to DNA damage. In the cellular response to DNA damage, the RPA complex controls DNA repair and DNA damage checkpoint activation. Through recruitment of ATRIP activates the ATR kinase a master regulator of the DNA damage response. It is required for the recruitment of the DNA double-strand break repair factors RAD51 and RAD52 to chromatin in response to DNA damage. Also recruits to sites of DNA damage proteins like XPA and XPG that are involved in nucleotide excision repair and is required for this mechanism of DNA repair. Also plays a role in base excision repair (BER) probably through interaction with UNG. Also recruits SMARCAL1/HARP, which is involved in replication fork restart, to sites of DNA damage. May also play a role in telomere maintenance. RPA stimulates 5'-3' helicase activity of BRIP1/FANCJ (PubMed : 17596542).
See full target information RPA2 phospho S33

Publications (15)

Recent publications for all applications. Explore the full list and refine your search

Communications biology 8:1011 PubMed40617868

2025

TTK activates ATR through RPA2 phosphorylation to promote olaparib resistance in ovarian cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Gonghua Qi,Hanlin Ma,Jingying Chen,Panpan Gai,Kai Teng

Nature communications 16:4931 PubMed40436896

2025

BH3 mimetics targeting BCL-XL have efficacy in solid tumors with RB1 loss and replication stress.

Applications

Unspecified application

Species

Unspecified reactive species

Andreas Varkaris,Keshan Wang,Mannan Nouri,Nina Kozlova,Daniel R Schmidt,Anastasia Stavridi,Seiji Arai,Nicholas Ambrosio,Larysa Poluben,Juan M Jimenez-Vacas,Daniel Westaby,Juliet Carmichael,Fang Xie,Ines Figueiredo,Lorenzo Buroni,Antje Neeb,Bora Gurel,Nicholas Chevalier,Lisha Brown,Olga Voznesensky,Shao-Yong Chen,Joshua W Russo,Xin Yuan,Dejan Juric,Himisha Beltran,Johann S De Bono,Matthew G Vander Heiden,David J Einstein,Taru Muranen,Eva Corey,Adam Sharp,Steven P Balk

Aging and disease : PubMed40423641

2025

Anillin Recedes in p53-Dependent Senescence of Tumor Cells and Reappears in Cells Escaping from Senescence.

Applications

Unspecified application

Species

Unspecified reactive species

Tomasz Buko,Karolina Staniak,Magdalena Dudkowska,Dorota Janiszewska,Dominika Dębowska,Agnieszka Gadecka,Anna Bielak-Zmijewska

Nature 637:402-411 PubMed39633051

2024

Fetal hepatocytes protect the HSPC genome via fetuin-A.

Applications

Unspecified application

Species

Unspecified reactive species

Xiao-Lin Guo,Yi-Ding Wang,Yan-Jun Liu,Lei Chu,Hua Zhu,Ye Hu,Ren-Yan Wu,Hong-Yu Xie,Juan Yu,Shui-Ping Li,Chao-Yang Xiong,Ruo-Yan Li,Fang Ke,Lei Chen,Guo-Qiang Chen,Liang Chen,Fan Bai,Tariq Enver,Guo-Hong Li,Huai-Fang Li,Deng-Li Hong

Nature communications 15:5237 PubMed38898005

2024

Therapy-induced secretion of spliceosomal components mediates pro-survival crosstalk between ovarian cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Victoria O Shender,Ksenia S Anufrieva,Polina V Shnaider,Georgij P Arapidi,Marat S Pavlyukov,Olga M Ivanova,Irina K Malyants,Grigory A Stepanov,Evgenii Zhuravlev,Rustam H Ziganshin,Ivan O Butenko,Olga N Bukato,Ksenia M Klimina,Vladimir A Veselovsky,Tatiana V Grigorieva,Sergey Y Malanin,Olga I Aleshikova,Andrey V Slonov,Nataliya A Babaeva,Lev A Ashrafyan,Elena Khomyakova,Evgeniy G Evtushenko,Maria M Lukina,Zixiang Wang,Artemiy S Silantiev,Anna A Nushtaeva,Daria D Kharlampieva,Vassili N Lazarev,Arseniy I Lashkin,Lorine K Arzumanyan,Irina Yu Petrushanko,Alexander A Makarov,Olga S Lebedeva,Alexandra N Bogomazova,Maria A Lagarkova,Vadim M Govorun

Molecular cell 84:1475-1495.e18 PubMed38521065

2024

Transcription elongation defects link oncogenic SF3B1 mutations to targetable alterations in chromatin landscape.

Applications

Unspecified application

Species

Unspecified reactive species

Prajwal C Boddu,Abhishek K Gupta,Rahul Roy,Bárbara De La Peña Avalos,Anne Olazabal-Herrero,Nils Neuenkirchen,Joshua T Zimmer,Namrata S Chandhok,Darren King,Yasuhito Nannya,Seishi Ogawa,Haifan Lin,Matthew D Simon,Eloise Dray,Gary M Kupfer,Amit Verma,Karla M Neugebauer,Manoj M Pillai

Nucleic acids research 51:12242-12260 PubMed37930826

2023

The RNA-binding motif protein 14 regulates telomere integrity at the interface of TERRA and telomeric R-loops.

Applications

Unspecified application

Species

Unspecified reactive species

Yajun Wang,Wei Zhu,Yumi Jang,Joshua A Sommers,Gong Yi,Chandrakala Puligilla,Deborah L Croteau,Yibin Yang,Mihoko Kai,Yie Liu

Cancer research 83:2750-2762 PubMed37306706

2023

Temozolomide Sensitizes ARID1A-Mutated Cancers to PARP Inhibitors.

Applications

Unspecified application

Species

Unspecified reactive species

Zheng-Cheng Yu,Tianhe Li,Ellen Tully,Peng Huang,Chih-Ning Chen,Philipp Oberdoerffer,Stephanie Gaillard,Ie-Ming Shih,Tian-Li Wang

Signal transduction and targeted therapy 7:354 PubMed36253371

2022

A small molecule inhibitor of the UBE2F-CRL5 axis induces apoptosis and radiosensitization in lung cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Tiantian Xu,Qisheng Ma,Yanan Li,Qing Yu,Peichen Pan,Yawen Zheng,Zhijian Li,Xiufang Xiong,Tingjun Hou,Bin Yu,Hongmin Liu,Yi Sun

iScience 25:104591 PubMed35789855

2022

A small-molecule Skp1 inhibitor elicits cell death by p53-dependent mechanism.

Applications

Unspecified application

Species

Unspecified reactive species

Muzammal Hussain,Yongzhi Lu,Muqddas Tariq,Hao Jiang,Yahai Shu,Shuang Luo,Qiang Zhu,Jiancun Zhang,Jinsong Liu
View all publications

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