Rabbit Polyclonal RPA32/RPA2 phospho S4 + S8 antibody. Suitable for IHC-P and reacts with Mouse, Human samples. Cited in 19 publications. Immunogen corresponding to Synthetic Peptide within Human RPA2 phospho S4 + S8.
Images
Publications
- Nature communications 13:51332022Crosstalk between SUMOylation and ubiquitylation controls DNA end resection by maintaining MRE11 homeostasis on chromatin.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTao Zhang,Han Yang,Zenan Zhou,Yongtai Bai,Jiadong Wang,Weibin WangPubMed 36050397
- Nature communications 13:42852022DNA nicks induce mutational signatures associated with BRCA1 deficiency.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYi-Li Feng,Qian Liu,Ruo-Dan Chen,Si-Cheng Liu,Zhi-Cheng Huang,Kun-Ming Liu,Xiao-Ying Yang,An-Yong XiePubMed 35879372
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 6.8 - 7.4
Preservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA- Form
Liquid
- Clonality
Polyclonal
Immunogen
- Synthetic Peptide within Human RPA2 phospho S4 + S8. The exact immunogen used to generate this antibody is proprietary information. Database link P15927
Reactivity data
Select an application| IHC-P | |
|---|---|
| Human | Tested |
| Mouse | Tested |
| Bat | Predicted |
| Gorilla | Predicted |
| Orangutan | Predicted |
| Rhesus monkey | Predicted |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/100.00000 - 1/500.00000 | Notes Epitope exposure with tris-EDTA pH9.0 buffer will enhance staining. |
Species Human | Dilution info 1/100.00000 - 1/500.00000 | Notes Epitope exposure with tris-EDTA pH9.0 buffer will enhance staining. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rhesus monkey, Gorilla, Bat, Orangutan | Dilution info - | Notes - |
Associated Products
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Target data
Function
As part of the heterotrimeric replication protein A complex (RPA/RP-A), binds and stabilizes single-stranded DNA intermediates, that form during DNA replication or upon DNA stress. It prevents their reannealing and in parallel, recruits and activates different proteins and complexes involved in DNA metabolism. Thereby, it plays an essential role both in DNA replication and the cellular response to DNA damage. In the cellular response to DNA damage, the RPA complex controls DNA repair and DNA damage checkpoint activation. Through recruitment of ATRIP activates the ATR kinase a master regulator of the DNA damage response. It is required for the recruitment of the DNA double-strand break repair factors RAD51 and RAD52 to chromatin in response to DNA damage. Also recruits to sites of DNA damage proteins like XPA and XPG that are involved in nucleotide excision repair and is required for this mechanism of DNA repair. Plays also a role in base excision repair (BER) probably through interaction with UNG. Also recruits SMARCAL1/HARP, which is involved in replication fork restart, to sites of DNA damage. May also play a role in telomere maintenance.
Alternative names
Replication protein A 32 kDa subunit, RP-A p32, Replication factor A protein 2, Replication protein A 34 kDa subunit, RF-A protein 2, RP-A p34, RPA34, RPA32, REPA2, RPA2
Recommended products
Rabbit Polyclonal RPA32/RPA2 phospho S4 + S8 antibody. Suitable for IHC-P and reacts with Mouse, Human samples. Cited in 19 publications. Immunogen corresponding to Synthetic Peptide within Human RPA2 phospho S4 + S8.
Alternative names
Replication protein A 32 kDa subunit, RP-A p32, Replication factor A protein 2, Replication protein A 34 kDa subunit, RF-A protein 2, RP-A p34, RPA34, RPA32, REPA2, RPA2
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 6.8 - 7.4
Preservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA- Form
Liquid
- Clonality
Polyclonal
- Immunogen
- Synthetic Peptide within Human RPA2 phospho S4 + S8. The exact immunogen used to generate this antibody is proprietary information. Database link P15927
- Purification technique
Affinity purification Immunogen
- Concentration
- Purification notes
Affinity purified using the peptide immobilized on solid support.
Storage
- Shipped at conditions
Blue Ice
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
Concentration is optimized for IHC and not determined
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Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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3 product images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPA32/RPA2 (phospho S4 + S8) antibody (ab87277)
Immunohistochemical analysis of formalin-fixed, paraffin-embedded human ovarian cancer tissue, labeling RPA32 with ab87277 at a 1/250 dilution. Detection: DAB.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPA32/RPA2 (phospho S4 + S8) antibody (ab87277)
Immunohistochemical analysis of formalin-fixed, paraffin-embedded mouse renal cancer tissue, labeling RPA32 with ab87277 at a 1/250 dilution. Detection: DAB.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPA32/RPA2 (phospho S4 + S8) antibody (ab87277)
Immunohistochemistry analysis of Human breast carcinoma using ab87277 at 1/100 dilution. Detection: Red-fluorescent goat anti-rabbit IgG at 1/100 dilution.
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