Rabbit Polyclonal RPA32/RPA2 phospho S4 + S8 antibody. Suitable for IHC-P and reacts with Mouse, Human samples. Cited in 19 publications. Immunogen corresponding to Synthetic Peptide within Human RPA2 phospho S4 + S8.
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- Nature communications 13:51332022Crosstalk between SUMOylation and ubiquitylation controls DNA end resection by maintaining MRE11 homeostasis on chromatin.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTao Zhang,Han Yang,Zenan Zhou,Yongtai Bai,Jiadong Wang,Weibin WangPubMed 36050397
- Nature communications 13:42852022DNA nicks induce mutational signatures associated with BRCA1 deficiency.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYi-Li Feng,Qian Liu,Ruo-Dan Chen,Si-Cheng Liu,Zhi-Cheng Huang,Kun-Ming Liu,Xiao-Ying Yang,An-Yong XiePubMed 35879372
- Cancer research 82:2156-21702022Tumor Intrinsic PD-L1 Promotes DNA Repair in Distinct Cancers and Suppresses PARP Inhibitor-Induced Synthetic Lethality.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAnand V R Kornepati,Jacob T Boyd,Clare E Murray,Julia Saifetiarova,Bárbara de la Peña Avalos,Cody M Rogers,Haiyan Bai,Alvaro S Padron,Yiji Liao,Carlos Ontiveros,Robert S Svatek,Robert Hromas,Rong Li,Yanfen Hu,Jose R Conejo-Garcia,Ratna K Vadlamudi,Weixing Zhao,Eloïse Dray,Patrick Sung,Tyler J CurielPubMed 35247877
- Nature 601:268-2732021HELQ is a dual-function DSB repair enzyme modulated by RPA and RAD51.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRoopesh Anand,Erika Buechelmaier,Ondrej Belan,Matthew Newton,Aleksandra Vancevska,Artur Kaczmarczyk,Tohru Takaki,David S Rueda,Simon N Powell,Simon J BoultonPubMed 34937945
- Molecular and cellular biology 42:e00524212021Replication Protein A Phosphorylation Facilitates RAD52-Dependent Homologous Recombination in BRCA-Deficient Cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAlison C Carley,Manisha Jalan,Shyamal Subramanyam,Rohini Roy,Gloria E O Borgstahl,Simon N PowellPubMed 34928169
- Molecular cell 81:4243-4257.e62021A basal-level activity of ATR links replication fork surveillance and stress response.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYandong Yin,Wei Ting Chelsea Lee,Dipika Gupta,Huijun Xue,Peter Tonzi,James A Borowiec,Tony T Huang,Mauro Modesti,Eli RothenbergPubMed 34473946
- Frontiers in cell and developmental biology 9:7148682021RECQ1 Promotes Stress Resistance and DNA Replication Progression Through PARP1 Signaling Pathway in Glioblastoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJing Zhang,Hao Lian,Kui Chen,Ying Pang,Mu Chen,Bingsong Huang,Lei Zhu,Siyi Xu,Min Liu,Chunlong ZhongPubMed 34381789
- Cell reports 35:1091532021p97/VCP inhibition causes excessive MRE11-dependent DNA end resection promoting cell killing after ionizing radiation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSusan Kilgas,Abhay Narayan Singh,Salome Paillas,Chee-Kin Then,Ignacio Torrecilla,Judith Nicholson,Lisa Browning,Iolanda Vendrell,Rebecca Konietzny,Benedikt M Kessler,Anne E Kiltie,Kristijan RamadanPubMed 34038735
- Cancer discovery 11:1754-17732021A Functional Taxonomy of Tumor Suppression in Oncogenic KRAS-Driven Lung Cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHongchen Cai,Su Kit Chew,Chuan Li,Min K Tsai,Laura Andrejka,Christopher W Murray,Nicholas W Hughes,Emily G Shuldiner,Emily L Ashkin,Rui Tang,King L Hung,Leo C Chen,Shi Ya C Lee,Maryam Yousefi,Wen-Yang Lin,Christian A Kunder,Le Cong,Christopher D McFarland,Dmitri A Petrov,Charles Swanton,Monte M WinslowPubMed 33608386
- Molecular cell 81:426-441.e82021Human DDK rescues stalled forks and counteracts checkpoint inhibition at unfired origins to complete DNA replication.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMathew J K Jones,Camille Gelot,Stephanie Munk,Amnon Koren,Yoshitaka Kawasoe,Kelly A George,Ruth E Santos,Jesper V Olsen,Steven A McCarroll,Mark G Frattini,Tatsuro S Takahashi,Prasad V JallepalliPubMed 33545059
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 6.8 - 7.4
Preservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human RPA2 phospho S4 + S8. The exact immunogen used to generate this antibody is proprietary information. Database link P15927
Reactivity data
Select an application| IHC-P | |
|---|---|
| Human | Tested |
| Mouse | Tested |
| Bat | Predicted |
| Gorilla | Predicted |
| Orangutan | Predicted |
| Rhesus monkey | Predicted |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/100.00000 - 1/500.00000 | Notes Epitope exposure with tris-EDTA pH9.0 buffer will enhance staining. |
Species Human | Dilution info 1/100.00000 - 1/500.00000 | Notes Epitope exposure with tris-EDTA pH9.0 buffer will enhance staining. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rhesus monkey, Gorilla, Bat, Orangutan | Dilution info - | Notes - |
Associated Products
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Target data
Function
As part of the heterotrimeric replication protein A complex (RPA/RP-A), binds and stabilizes single-stranded DNA intermediates that form during DNA replication or upon DNA stress. It prevents their reannealing and in parallel, recruits and activates different proteins and complexes involved in DNA metabolism. Thereby, it plays an essential role both in DNA replication and the cellular response to DNA damage. In the cellular response to DNA damage, the RPA complex controls DNA repair and DNA damage checkpoint activation. Through recruitment of ATRIP activates the ATR kinase a master regulator of the DNA damage response. It is required for the recruitment of the DNA double-strand break repair factors RAD51 and RAD52 to chromatin in response to DNA damage. Also recruits to sites of DNA damage proteins like XPA and XPG that are involved in nucleotide excision repair and is required for this mechanism of DNA repair. Also plays a role in base excision repair (BER) probably through interaction with UNG. Also recruits SMARCAL1/HARP, which is involved in replication fork restart, to sites of DNA damage. May also play a role in telomere maintenance. RPA stimulates 5'-3' helicase activity of BRIP1/FANCJ (PubMed:17596542).
Alternative names
REPA2, RPA32, RPA34, RPA2, Replication protein A 32 kDa subunit, RP-A p32, Replication factor A protein 2, Replication protein A 34 kDa subunit, RF-A protein 2, RP-A p34
Recommended products
Rabbit Polyclonal RPA32/RPA2 phospho S4 + S8 antibody. Suitable for IHC-P and reacts with Mouse, Human samples. Cited in 19 publications. Immunogen corresponding to Synthetic Peptide within Human RPA2 phospho S4 + S8.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 6.8 - 7.4
Preservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human RPA2 phospho S4 + S8. The exact immunogen used to generate this antibody is proprietary information. Database link P15927
- Purification technique
- Affinity purification Immunogen
- Concentration
- Purification notes
Affinity purified using the peptide immobilized on solid support.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Concentration is optimized for IHC and not determined
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
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3 product images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPA32/RPA2 (phospho S4 + S8) antibody (ab87277)
Immunohistochemical analysis of formalin-fixed, paraffin-embedded human ovarian cancer tissue, labeling RPA32 with ab87277 at a 1/250 dilution. Detection: DAB.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPA32/RPA2 (phospho S4 + S8) antibody (ab87277)
Immunohistochemical analysis of formalin-fixed, paraffin-embedded mouse renal cancer tissue, labeling RPA32 with ab87277 at a 1/250 dilution. Detection: DAB.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPA32/RPA2 (phospho S4 + S8) antibody (ab87277)
Immunohistochemistry analysis of Human breast carcinoma using ab87277 at 1/100 dilution. Detection: Red-fluorescent goat anti-rabbit IgG at 1/100 dilution.
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