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Rabbit Recombinant Monoclonal RPA32/RPA2 phospho S4 + S8 antibody. Suitable for ICC, IP, WB and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human RPA2 phospho S4 + S8.

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Images

Immunoprecipitation - Anti-RPA32/RPA2 (phospho S4 + S8) antibody [BL-165-5F1] (AB243866), expandable thumbnail
  • Immunocytochemistry - Anti-RPA32/RPA2 (phospho S4 + S8) antibody [BL-165-5F1] (AB243866), expandable thumbnail
  • Western blot - Anti-RPA32/RPA2 (phospho S4 + S8) antibody [BL-165-5F1] (AB243866), expandable thumbnail

Publications

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.8 - 8.6
Preservative: 0.09% Sodium azide
Constituents: 99% Borate buffered saline

Form

Liquid

Clonality

Monoclonal

Immunogen

  • Synthetic Peptide within Human RPA2 phospho S4 + S8. The exact immunogen used to generate this antibody is proprietary information. Database link P15927

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
ICCIPWB
Human
Tested
Tested
Tested

Tested
Tested

Species

Human

Dilution info

1/5000

Notes

-

Tested
Tested

Species

Human

Dilution info

-

Notes

Use 5μl/0.5mg lysate.

Tested
Tested

Species

Human

Dilution info

1/1000

Notes

-

Associated Products

Select an associated product type

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Target data

Function

As part of the heterotrimeric replication protein A complex (RPA/RP-A), binds and stabilizes single-stranded DNA intermediates that form during DNA replication or upon DNA stress. It prevents their reannealing and in parallel, recruits and activates different proteins and complexes involved in DNA metabolism. Thereby, it plays an essential role both in DNA replication and the cellular response to DNA damage. In the cellular response to DNA damage, the RPA complex controls DNA repair and DNA damage checkpoint activation. Through recruitment of ATRIP activates the ATR kinase a master regulator of the DNA damage response. It is required for the recruitment of the DNA double-strand break repair factors RAD51 and RAD52 to chromatin in response to DNA damage. Also recruits to sites of DNA damage proteins like XPA and XPG that are involved in nucleotide excision repair and is required for this mechanism of DNA repair. Also plays a role in base excision repair (BER) probably through interaction with UNG. Also recruits SMARCAL1/HARP, which is involved in replication fork restart, to sites of DNA damage. May also play a role in telomere maintenance. RPA stimulates 5'-3' helicase activity of BRIP1/FANCJ (PubMed:17596542).

Alternative names

Recommended products

Rabbit Recombinant Monoclonal RPA32/RPA2 phospho S4 + S8 antibody. Suitable for ICC, IP, WB and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human RPA2 phospho S4 + S8.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • Synthetic Peptide within Human RPA2 phospho S4 + S8. The exact immunogen used to generate this antibody is proprietary information. Database link P15927
Clone number

BL-165-5F1

Concentration
Loading...
Purification notes

Recombinant antibody was purified from cell culture supernatant.

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

This product is sold under License from Bethyl Laboratories, Inc.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

3 product images

  • Immunoprecipitation - Anti-RPA32/RPA2 (phospho S4 + S8) antibody [BL-165-5F1] (ab243866), expandable thumbnail

    Immunoprecipitation - Anti-RPA32/RPA2 (phospho S4 + S8) antibody [BL-165-5F1] (ab243866)

    RPA32/RPA2 was immunoprecipitated from HeLa whole cell lysate (0.5 mg per IP reaction, 20% loaded) with ab243866 at 5 μg per reaction. Western blot was performed on the immunoprecipitate using ab243866 at 1/1000 dilution.

    Lane 1: ab243866 IP in HeLa whole cell lysate treated with etoposide

    Lane 2: ab243866 IP in HeLa whole cell lysate mock-treated

    Lane 3: rabbit anti-RPA32 antibody IP in HeLa whole cell lysate treated with etoposide

    Lane 4: rabbit anti-RPA32 antibody IP in HeLa whole cell lysate mock-treated

    Lane 5: Control IgG in HeLa whole cell lysate treated with etoposide

    Detection: Chemiluminescence with an exposure time of 3 minutes

    All lanes: Immunoprecipitation - Anti-RPA32/RPA2 (phospho S4 + S8) antibody [BL-165-5F1] (ab243866)

    Predicted band size: 29 kDa

    Exposure time: 3min

  • Immunocytochemistry - Anti-RPA32/RPA2 (phospho S4 + S8) antibody [BL-165-5F1] (ab243866), expandable thumbnail

    Immunocytochemistry - Anti-RPA32/RPA2 (phospho S4 + S8) antibody [BL-165-5F1] (ab243866)

    NBF-fixed HeLa cells treated with etoposide (right) or mock treated (left) stained for RPA32/RPA2 (phospho S4 + S8) using ab243866 at 1/5000 dilution. Goat Anti-Rabbit IgG H&L (DyLight® 594) (Goat Anti-Rabbit IgG H&L (DyLight® 594) ab96885) was used as a secondary antibody. Counterstained with DAPI (blue).

  • Western blot - Anti-RPA32/RPA2 (phospho S4 + S8) antibody [BL-165-5F1] (ab243866), expandable thumbnail

    Western blot - Anti-RPA32/RPA2 (phospho S4 + S8) antibody [BL-165-5F1] (ab243866)

    Western blot analysis of pRPA32 (S4/S8) using ab243866 at 1/1000 dilution.

    Lane 1: HeLa cell lysate treated with 100 µM etoposide for 4 hours.

    Lane 2: HeLa cell lysate negetive treatment.

    A HRP-conjugated goat anti-rabbit IgG was used as the secondary antibody. Detection: Chemiluminescence with an exposure time of 10 seconds.

    All lanes: Western blot - Anti-RPA32/RPA2 (phospho S4 + S8) antibody [BL-165-5F1] (ab243866)

    Predicted band size: 29 kDa

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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