Rabbit Recombinant Monoclonal RPA32/RPA2 phospho S4 + S8 antibody. Suitable for ICC, IP, WB and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human RPA2 phospho S4 + S8.
IgG
Rabbit
pH: 7.8 - 8.6
Preservative: 0.09% Sodium azide
Constituents: 99% Borate buffered saline
Liquid
Monoclonal
ICC | IP | WB | |
---|---|---|---|
Human | Tested | Tested | Tested |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/5000 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info - | Notes Use 5μl/0.5mg lysate. |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/1000 | Notes - |
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As part of the heterotrimeric replication protein A complex (RPA/RP-A), binds and stabilizes single-stranded DNA intermediates that form during DNA replication or upon DNA stress. It prevents their reannealing and in parallel, recruits and activates different proteins and complexes involved in DNA metabolism. Thereby, it plays an essential role both in DNA replication and the cellular response to DNA damage. In the cellular response to DNA damage, the RPA complex controls DNA repair and DNA damage checkpoint activation. Through recruitment of ATRIP activates the ATR kinase a master regulator of the DNA damage response. It is required for the recruitment of the DNA double-strand break repair factors RAD51 and RAD52 to chromatin in response to DNA damage. Also recruits to sites of DNA damage proteins like XPA and XPG that are involved in nucleotide excision repair and is required for this mechanism of DNA repair. Also plays a role in base excision repair (BER) probably through interaction with UNG. Also recruits SMARCAL1/HARP, which is involved in replication fork restart, to sites of DNA damage. May also play a role in telomere maintenance. RPA stimulates 5'-3' helicase activity of BRIP1/FANCJ (PubMed:17596542).
REPA2, RPA32, RPA34, RPA2, Replication protein A 32 kDa subunit, RP-A p32, Replication factor A protein 2, Replication protein A 34 kDa subunit, RF-A protein 2, RP-A p34
Rabbit Recombinant Monoclonal RPA32/RPA2 phospho S4 + S8 antibody. Suitable for ICC, IP, WB and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human RPA2 phospho S4 + S8.
IgG
Rabbit
pH: 7.8 - 8.6
Preservative: 0.09% Sodium azide
Constituents: 99% Borate buffered saline
Liquid
Monoclonal
BL-165-5F1
Recombinant antibody was purified from cell culture supernatant.
Blue Ice
1-2 weeks
+4°C
-20°C
Upon delivery aliquot
Avoid freeze / thaw cycle
This product is sold under License from Bethyl Laboratories, Inc.
We have tested this species and application combination and it works. It is covered by our product promise.
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
We do not recommend this combination. It is not covered by our product promise.
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Full details and terms and conditions can be found here:
Terms & Conditions.
RPA32/RPA2 was immunoprecipitated from HeLa whole cell lysate (0.5 mg per IP reaction, 20% loaded) with ab243866 at 5 μg per reaction. Western blot was performed on the immunoprecipitate using ab243866 at 1/1000 dilution.
Lane 1: ab243866 IP in HeLa whole cell lysate treated with etoposide
Lane 2: ab243866 IP in HeLa whole cell lysate mock-treated
Lane 3: rabbit anti-RPA32 antibody IP in HeLa whole cell lysate treated with etoposide
Lane 4: rabbit anti-RPA32 antibody IP in HeLa whole cell lysate mock-treated
Lane 5: Control IgG in HeLa whole cell lysate treated with etoposide
Detection: Chemiluminescence with an exposure time of 3 minutes
All lanes: Immunoprecipitation - Anti-RPA32/RPA2 (phospho S4 + S8) antibody [BL-165-5F1] (ab243866)
Predicted band size: 29 kDa
Exposure time: 3min
NBF-fixed HeLa cells treated with etoposide (right) or mock treated (left) stained for RPA32/RPA2 (phospho S4 + S8) using ab243866 at 1/5000 dilution. Goat Anti-Rabbit IgG H&L (DyLight® 594) (Goat Anti-Rabbit IgG H&L (DyLight® 594) ab96885) was used as a secondary antibody. Counterstained with DAPI (blue).
Western blot analysis of pRPA32 (S4/S8) using ab243866 at 1/1000 dilution.
Lane 1: HeLa cell lysate treated with 100 µM etoposide for 4 hours.
Lane 2: HeLa cell lysate negetive treatment.
A HRP-conjugated goat anti-rabbit IgG was used as the secondary antibody. Detection: Chemiluminescence with an exposure time of 10 seconds.
All lanes: Western blot - Anti-RPA32/RPA2 (phospho S4 + S8) antibody [BL-165-5F1] (ab243866)
Predicted band size: 29 kDa
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