AB243866

Anti-RPA32/RPA2 (phospho S4 + S8) antibody [BL-165-5F1]

Recombinant
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(6 Publications)

Rabbit Recombinant Monoclonal RPA32/RPA2 phospho S4 + S8 antibody. Suitable for ICC, IP, WB and reacts with Human samples. Cited in 6 publications. Immunogen corresponding to Synthetic Peptide within Human RPA2 phospho S4 + S8.

View Alternative Names

REPA2, RPA32, RPA34, RPA2, Replication protein A 32 kDa subunit, RP-A p32, Replication factor A protein 2, Replication protein A 34 kDa subunit, RF-A protein 2, RP-A p34

3 Images

Supplier Data

Immunocytochemistry - Anti-RPA32/RPA2 (phospho S4 + S8) antibody [BL-165-5F1] (AB243866)

NBF-fixed HeLa cells treated with etoposide (right) or mock treated (left) stained for RPA32/RPA2 (phospho S4 + S8) using ab243866 at 1/5000 dilution. Goat Anti-Rabbit IgG H&L (DyLight^® 594) (ab96885) was used as a secondary antibody. Counterstained with DAPI (blue).

Supplier Data

Immunoprecipitation - Anti-RPA32/RPA2 (phospho S4 + S8) antibody [BL-165-5F1] (AB243866)

RPA32/RPA2 was immunoprecipitated from HeLa whole cell lysate (0.5 mg per IP reaction, 20% loaded) with ab243866 at 5 μg per reaction. Western blot was performed on the immunoprecipitate using ab243866 at 1/1000 dilution.

Lane 1 : ab243866 IP in HeLa whole cell lysate treated with etoposide

Lane 2 : ab243866 IP in HeLa whole cell lysate mock-treated

Lane 3 : rabbit anti-RPA32 antibody IP in HeLa whole cell lysate treated with etoposide

Lane 4 : rabbit anti-RPA32 antibody IP in HeLa whole cell lysate mock-treated

Lane 5 : Control IgG in HeLa whole cell lysate treated with etoposide

Detection : Chemiluminescence with an exposure time of 3 minutes

All lanes:

Immunoprecipitation - Anti-RPA32/RPA2 (phospho S4 + S8) antibody [BL-165-5F1] (ab243866)

Predicted band size: 29 kDa

false

Exposure time: 3min

Unknown

Western blot - Anti-RPA32/RPA2 (phospho S4 + S8) antibody [BL-165-5F1] (AB243866)

Western blot analysis of pRPA32 (S4/S8) using ab243866 at 1/1000 dilution.

Lane 1 : HeLa cell lysate treated with 100 µM etoposide for 4 hours.

Lane 2 : HeLa cell lysate negetive treatment.

A HRP-conjugated goat anti-rabbit IgG was used as the secondary antibody. Detection : Chemiluminescence with an exposure time of 10 seconds.

All lanes:

Western blot - Anti-RPA32/RPA2 (phospho S4 + S8) antibody [BL-165-5F1] (ab243866)

Predicted band size: 29 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

BL-165-5F1

Isotype

IgG

Carrier free

Reacts with

Human

Applications

ICC, IP, WB

applications

Immunogen

Synthetic Peptide within Human RPA2 phospho S4 + S8. The exact immunogen used to generate this antibody is proprietary information.

P15927

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICC" : {"fullname" : "Immunocytochemistry", "shortname":"ICC"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICC-species-checked": "testedAndGuaranteed", "ICC-species-dilution-info": "1/5000", "ICC-species-notes": "", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "Use 5μl/0.5mg lysate.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "" } } }

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Product details

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

This product is sold under License from Bethyl Laboratories, Inc.

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Properties and storage information

Form

Liquid

Purification notes

Recombinant antibody was purified from cell culture supernatant.

Storage buffer

pH: 7.8 - 8.6 Preservative: 0.09% Sodium azide Constituents: 99% Borate buffered saline

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

As part of the heterotrimeric replication protein A complex (RPA/RP-A), binds and stabilizes single-stranded DNA intermediates that form during DNA replication or upon DNA stress. It prevents their reannealing and in parallel, recruits and activates different proteins and complexes involved in DNA metabolism. Thereby, it plays an essential role both in DNA replication and the cellular response to DNA damage. In the cellular response to DNA damage, the RPA complex controls DNA repair and DNA damage checkpoint activation. Through recruitment of ATRIP activates the ATR kinase a master regulator of the DNA damage response. It is required for the recruitment of the DNA double-strand break repair factors RAD51 and RAD52 to chromatin in response to DNA damage. Also recruits to sites of DNA damage proteins like XPA and XPG that are involved in nucleotide excision repair and is required for this mechanism of DNA repair. Also plays a role in base excision repair (BER) probably through interaction with UNG. Also recruits SMARCAL1/HARP, which is involved in replication fork restart, to sites of DNA damage. May also play a role in telomere maintenance. RPA stimulates 5'-3' helicase activity of BRIP1/FANCJ (PubMed : 17596542).

See full target information RPA2 phospho S4 + S8

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Publications (6)

Recent publications for all applications. Explore the full list and refine your search

Communications biology 8:1011 PubMed40617868

2025

TTK activates ATR through RPA2 phosphorylation to promote olaparib resistance in ovarian cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Gonghua Qi,Hanlin Ma,Jingying Chen,Panpan Gai,Kai Teng

British journal of cancer 133:381-393 PubMed40442403

2025

Sequential ATR and PARP inhibition overcomes acquired DNA damaging agent resistance in pancreatic ductal adenocarcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Katharine J Herbert,Rosie Upstill-Goddard,Stephan B Dreyer,Selma Rebus,Christian Pilarsky,Mukhopadhyay Debabrata,Christopher J Lord,Andrew V Biankin,Fieke E M Froeling,David K Chang

Cell discovery 9:47 PubMed37156794

2023

BRD4-targeting PROTAC as a unique tool to study biomolecular condensates.

Applications

Unspecified application

Species

Unspecified reactive species

Yi Shi,Yuan Liao,Qianlong Liu,Zhihao Ni,Zhenzhen Zhang,Minglei Shi,Pilong Li,Haitao Li,Yu Rao

Cell biology international 46:1196-1203 PubMed35661479

2022

MCL-1 interacts with MOF and BID to regulate H4K16 acetylation and homologous recombination repair.

Applications

Unspecified application

Species

Unspecified reactive species

Abid R Mattoo,John M Jessup

Nucleic acids research 49:7492-7506 PubMed34197599

2021

Replication catastrophe induced by cyclic hypoxia leads to increased APOBEC3B activity.

Applications

Unspecified application

Species

Unspecified reactive species

Samuel B Bader,Tiffany S Ma,Charlotte J Simpson,Jiachen Liang,Sakura Eri B Maezono,Monica M Olcina,Francesca M Buffa,Ester M Hammond

American journal of translational research 13:601-616 PubMed33594312

2021

LACTB induced apoptosis of oxaliplatin-resistant gastric cancer through regulating autophagy-mediated mitochondrial apoptosis pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Fang Yang,Zhiqiang Yan,Wei Nie,Xingzhen Cheng,Zeying Liu,Wei Wang,Chunyan Shao,Gui Fu,Yanni Yu

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-RPA32/RPA2 (phospho S4 + S8) antibody [BL-165-5F1]

Immunocytochemistry - Anti-RPA32/RPA2 (phospho S4 + S8) antibody [BL-165-5F1] (AB243866)

Immunoprecipitation - Anti-RPA32/RPA2 (phospho S4 + S8) antibody [BL-165-5F1] (AB243866)

Western blot - Anti-RPA32/RPA2 (phospho S4 + S8) antibody [BL-165-5F1] (AB243866)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (6)

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