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Rabbit Polyclonal RPA32/RPA2 phospho T21 antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 23 publications. Immunogen corresponding to Synthetic Peptide within Human RPA2 phospho T21 aa 1-100.


Images

Western blot - Anti-RPA32/RPA2 (phospho T21) antibody (AB61065), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPA32/RPA2 (phospho T21) antibody (AB61065), expandable thumbnail

Publications

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride

Form

Liquid

Clonality

Polyclonal

Immunogen

  • Synthetic Peptide within Human RPA2 phospho T21 aa 1-100. The exact immunogen used to generate this antibody is proprietary information. Database link P15927

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Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBIHC-P
Human
Tested
Tested

Tested
Tested

Species

Human

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

1/50 - 1/100

Notes

-

Associated Products

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Target data

Function

As part of the heterotrimeric replication protein A complex (RPA/RP-A), binds and stabilizes single-stranded DNA intermediates that form during DNA replication or upon DNA stress. It prevents their reannealing and in parallel, recruits and activates different proteins and complexes involved in DNA metabolism. Thereby, it plays an essential role both in DNA replication and the cellular response to DNA damage. In the cellular response to DNA damage, the RPA complex controls DNA repair and DNA damage checkpoint activation. Through recruitment of ATRIP activates the ATR kinase a master regulator of the DNA damage response. It is required for the recruitment of the DNA double-strand break repair factors RAD51 and RAD52 to chromatin in response to DNA damage. Also recruits to sites of DNA damage proteins like XPA and XPG that are involved in nucleotide excision repair and is required for this mechanism of DNA repair. Also plays a role in base excision repair (BER) probably through interaction with UNG. Also recruits SMARCAL1/HARP, which is involved in replication fork restart, to sites of DNA damage. May also play a role in telomere maintenance. RPA stimulates 5'-3' helicase activity of BRIP1/FANCJ (PubMed:17596542).

Alternative names

Recommended products

Rabbit Polyclonal RPA32/RPA2 phospho T21 antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 23 publications. Immunogen corresponding to Synthetic Peptide within Human RPA2 phospho T21 aa 1-100.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Polyclonal

Immunogen
  • Synthetic Peptide within Human RPA2 phospho T21 aa 1-100. The exact immunogen used to generate this antibody is proprietary information. Database link P15927
Purification technique

Affinity purification Immunogen

Specificity

ab61065 detects endogenous levels of RPA32/RFA2 only when phosphorylated at threonine 21.

Concentration
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Purification notes

ab61065 was affinity-purified from rabbit antiserum by affinity chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Product promise

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In the unlikely event of one of our products not working as expected, you are covered by our product promise.

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2 product images

  • Western blot - Anti-RPA32/RPA2 (phospho T21) antibody (ab61065), expandable thumbnail

    Western blot - Anti-RPA32/RPA2 (phospho T21) antibody (ab61065)

    All lanes: Western blot - Anti-RPA32/RPA2 (phospho T21) antibody (ab61065) at 1/500 dilution

    All lanes: HeLa (Human epithelial cell line from cervix adenocarcinoma) cell lysates treated with Adriamycin 0.5 µg/ml 24 hours

    Developed using the ECL technique.

    Predicted band size: 29 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPA32/RPA2 (phospho T21) antibody (ab61065), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPA32/RPA2 (phospho T21) antibody (ab61065)

    Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue using ab61065 at 1/50 dilution.

    Left panel: Without immunizing phosphopeptide.

    Right panel: Plus immunizing phosphopeptide.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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