Rabbit Polyclonal RPA32/RPA2 phospho T21 antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 23 publications. Immunogen corresponding to Synthetic Peptide within Human RPA2 phospho T21 aa 1-100.
IgG
Rabbit
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
Liquid
Polyclonal
WB | IHC-P | |
---|---|---|
Human | Tested | Tested |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/50 - 1/100 | Notes - |
Select an associated product type
As part of the heterotrimeric replication protein A complex (RPA/RP-A), binds and stabilizes single-stranded DNA intermediates that form during DNA replication or upon DNA stress. It prevents their reannealing and in parallel, recruits and activates different proteins and complexes involved in DNA metabolism. Thereby, it plays an essential role both in DNA replication and the cellular response to DNA damage. In the cellular response to DNA damage, the RPA complex controls DNA repair and DNA damage checkpoint activation. Through recruitment of ATRIP activates the ATR kinase a master regulator of the DNA damage response. It is required for the recruitment of the DNA double-strand break repair factors RAD51 and RAD52 to chromatin in response to DNA damage. Also recruits to sites of DNA damage proteins like XPA and XPG that are involved in nucleotide excision repair and is required for this mechanism of DNA repair. Also plays a role in base excision repair (BER) probably through interaction with UNG. Also recruits SMARCAL1/HARP, which is involved in replication fork restart, to sites of DNA damage. May also play a role in telomere maintenance. RPA stimulates 5'-3' helicase activity of BRIP1/FANCJ (PubMed:17596542).
REPA2, RPA32, RPA34, RPA2, REPA2, RPA32, RPA34, Replication protein A 32 kDa subunit, RP-A p32, Replication factor A protein 2, Replication protein A 34 kDa subunit, RF-A protein 2, RP-A p34
Rabbit Polyclonal RPA32/RPA2 phospho T21 antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 23 publications. Immunogen corresponding to Synthetic Peptide within Human RPA2 phospho T21 aa 1-100.
IgG
Rabbit
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
Liquid
Polyclonal
Affinity purification Immunogen
ab61065 detects endogenous levels of RPA32/RFA2 only when phosphorylated at threonine 21.
ab61065 was affinity-purified from rabbit antiserum by affinity chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Blue Ice
+4°C
-20°C
Upon delivery aliquot
Avoid freeze / thaw cycle
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All lanes: Western blot - Anti-RPA32/RPA2 (phospho T21) antibody (ab61065) at 1/500 dilution
All lanes: HeLa (Human epithelial cell line from cervix adenocarcinoma) cell lysates treated with Adriamycin 0.5 µg/ml 24 hours
Developed using the ECL technique.
Predicted band size: 29 kDa
Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue using ab61065 at 1/50 dilution.
Left panel: Without immunizing phosphopeptide.
Right panel: Plus immunizing phosphopeptide.
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