AB109394

Anti-RPA32/RPA2 (phospho T21) antibody [EPR2846(2)]

Name: Anti-RPA32/RPA2 (phospho T21) antibody [EPR2846(2)] (ab109394) | Abcam
Brand: Abcam Limited
SKU: AB109394
Price: 520 USD
Availability: InStock
Rating: 4 (1 reviews)

RabMAb
Recombinant
What is this?

(1 Review)

(32 Publications)

Rabbit Recombinant Monoclonal RPA32/RPA2 phospho T21 antibody. Suitable for ELISA, Dot, WB and reacts with Recombinant fragment - Human, Mouse, Rat, Human samples. Cited in 32 publications.

View Alternative Names

REPA2, RPA32, RPA34, RPA2, Replication protein A 32 kDa subunit, RP-A p32, Replication factor A protein 2, Replication protein A 34 kDa subunit, RF-A protein 2, RP-A p34

8 Images

ELISA - Anti-RPA32/RPA2 (phospho T21) antibody [EPR2846(2)] (AB109394)

ELISA

Supplier Data

ELISA - Anti-RPA32/RPA2 (phospho T21) antibody [EPR2846(2)] (AB109394)

Direct ELISA antigen dose-response curve using purified ab109394.

Primary antibody : ab109394 (0-1000nweg/ml).

Antigens : P1 : Human RPA32/RPA2 (phospho T21) at 10ng/ml and 1ng/ml; NP : non-phospho at 10ng/ml and 1 ng/ml.

Secondary antibody : Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at 1/2500 dilution.

Supplier Data

Western blot - Anti-RPA32/RPA2 (phospho T21) antibody [EPR2846(2)] (AB109394)

Blocking/Diluting buffer and concentration : 5% NFDM/TBST

All lanes:

Western blot - Anti-RPA32/RPA2 (phospho T21) antibody [EPR2846(2)] (ab109394) at 1/5000 dilution

Lane 1:

MDA-MB-231 (Human breast adenocarcinoma epithelial cell) whole cell lysates at 15 µg

Lane 2:

MDA-MB-231 (Human breast adenocarcinoma epithelial cell) whole cell lysates. Then the membrane was incubated with phosphatase. at 15 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 29 kDa

Observed band size: 32 kDa,36 kDa

false

Exposure time: 30s

Lab

Western blot - Anti-RPA32/RPA2 (phospho T21) antibody [EPR2846(2)] (AB109394)

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-RPA32/RPA2 (phospho T21) antibody [EPR2846(2)] (ab109394) at 1/50000 dilution

Lane 1:

HeLa whole cell lysate - untreated at 10 µg

Lane 2:

HeLa whole cell lysate - treated with Calyculin A at 10 µg

Lane 3:

HeLa whole cell lysate - treated with Calyculin A and Alkaline Phosphatase at 10 µg

Secondary

All lanes:

Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

Predicted band size: 29 kDa

Observed band size: 32 kDa

false

Exposure time: 30s

Unknown

Western blot - Anti-RPA32/RPA2 (phospho T21) antibody [EPR2846(2)] (AB109394)

All lanes:

Western blot - Anti-RPA32/RPA2 (phospho T21) antibody [EPR2846(2)] (ab109394) at 1/50000 dilution

Lane 1:

HeLa cell lysates, untreated at 10 µg

Lane 2:

HeLa cell lysates, treated with Camptothecin at 10 µg

Predicted band size: 29 kDa

Observed band size: 32 kDa

false

Supplier Data

Dot Blot - Anti-RPA32/RPA2 (phospho T21) antibody [EPR2846(2)] (AB109394)

Dot blot analysis of human c-Myb RPA32/RPA2 (pT21) phospho peptide (Lane 1) and RPA32/RPA2 non-phospho peptide (Lane 2) labeling RPA32/RPA2 (phospho T21) with purified ab109394 at a dilution of 1/5000. ab97051 (Peroxidase conjugated goat anti-rabbit IgG (H+L)) was used as the secondary antibody at a dilution of 1/2500.

Blocking and dilution buffer : 5% NFDM/TBST.

Exposure time : 3 minutes.

Lab

Dot Blot - Anti-RPA32/RPA2 (phospho T21) antibody [EPR2846(2)] (AB109394)

Dot blot analysis of RPA32/RPA2 (pT21) phospho peptide (lane 1) and RPA32/RPA2 non-phospho peptide (lane 2) labelling RPA32/RPA2 (phospho T21) with ab109394 at a dilution of 1/1000. A peroxidase-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/2500).

Blocking and dilution buffer : 5% NFDM/TBST.

Exposure time : 3 minutes.

Supplier Data

Western blot - Anti-RPA32/RPA2 (phospho T21) antibody [EPR2846(2)] (AB109394)

Blocking/Diluting buffer and concentration : 5% NFDM/TBST.

Exposure time : 110 seconds.

All lanes:

Western blot - Anti-RPA32/RPA2 (phospho T21) antibody [EPR2846(2)] (ab109394) at 1/2000 dilution

Lane 1:

C6 (Rat glial tumor glial cell) whole cell lysates at 15 µg

Lane 2:

C6 (Rat glial tumor glial cell) treated with 100nM calyculin A for 60 minutes whole cell lysates at 15 µg

Lane 3:

C6 (Rat glial tumor glial cell) treated with 100nM calyculin A for 60 minutes whole cell lysates. Then the membrane was incubated with phosphatase at 15 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 29 kDa

Observed band size: 32 kDa,36 kDa

false

Supplier Data

Western blot - Anti-RPA32/RPA2 (phospho T21) antibody [EPR2846(2)] (AB109394)

Blocking/Diluting buffer and concentration : 5% NFDM/TBST.

Exposure time : 180 seconds.

All lanes:

Western blot - Anti-RPA32/RPA2 (phospho T21) antibody [EPR2846(2)] (ab109394) at 1/2000 dilution

Lane 1:

NIH/3T3 (Mouse embryonic fibroblast) whole cell lysates at 15 µg

Lane 2:

NIH/3T3 (Mouse embryonic fibroblast) treated with 100ng/ml calyculin A for 30 minutes whole cell lysates at 15 µg

Lane 3:

NIH/3T3 (Mouse embryonic fibroblast) treated with 100ng/ml calyculin A for 60 3minutes whole cell lysates.Then the membrane was incubated with phosphatase at 15 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 29 kDa

Observed band size: 32 kDa,36 kDa

false

Carrier free

Anti-RPA32/RPA2 (phospho T21) antibody [EPR2846(2)] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR2846(2)

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

ELISA, WB, Dot

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

ab109394 only detects RPA32/RPA2 phosphorylated at threonine 21. The antibody has been tested with milk (5%) and BSA (2%) as blocking reagents, but it provided better results with 5% milk.

This antibody cross-reactivates with non-phospho substrate in specific situations.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"}, "Dot" : {"fullname" : "Dot Blot", "shortname":"Dot"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "Dot-species-checked": "guaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/50000 - 1/200000", "WB-species-notes": "The antibody has been tested with milk (5%) and BSA (2%) as blocking reagents, but it provided better results with 5%milk.", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "1/500 - 1/1000", "ICCIF-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "1/250 - 1/500", "IHCP-species-notes": "Heat up to 98 °C, below boiling, and then let cool for 10-20 minutes." }, "Mouse": { "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "Dot-species-checked": "guaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/50000 - 1/200000", "WB-species-notes": "The antibody has been tested with milk (5%) and BSA (2%) as blocking reagents, but it provided better results with 5%milk.", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "1/500 - 1/1000", "ICCIF-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "1/250 - 1/500", "IHCP-species-notes": "Heat up to 98 °C, below boiling, and then let cool for 10-20 minutes." }, "Rat": { "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "Dot-species-checked": "guaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/50000 - 1/200000", "WB-species-notes": "The antibody has been tested with milk (5%) and BSA (2%) as blocking reagents, but it provided better results with 5%milk.", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "1/500 - 1/1000", "ICCIF-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "1/250 - 1/500", "IHCP-species-notes": "Heat up to 98 °C, below boiling, and then let cool for 10-20 minutes." }, "Recombinant fragment - Human": { "ELISA-species-checked": "testedAndGuaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "Dot-species-checked": "testedAndGuaranteed", "Dot-species-dilution-info": "1/1000", "Dot-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.05% Sodium azide Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 0.1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Stable for 12 months at -20°C

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

As part of the heterotrimeric replication protein A complex (RPA/RP-A), binds and stabilizes single-stranded DNA intermediates that form during DNA replication or upon DNA stress. It prevents their reannealing and in parallel, recruits and activates different proteins and complexes involved in DNA metabolism. Thereby, it plays an essential role both in DNA replication and the cellular response to DNA damage. In the cellular response to DNA damage, the RPA complex controls DNA repair and DNA damage checkpoint activation. Through recruitment of ATRIP activates the ATR kinase a master regulator of the DNA damage response. It is required for the recruitment of the DNA double-strand break repair factors RAD51 and RAD52 to chromatin in response to DNA damage. Also recruits to sites of DNA damage proteins like XPA and XPG that are involved in nucleotide excision repair and is required for this mechanism of DNA repair. Also plays a role in base excision repair (BER) probably through interaction with UNG. Also recruits SMARCAL1/HARP, which is involved in replication fork restart, to sites of DNA damage. May also play a role in telomere maintenance. RPA stimulates 5'-3' helicase activity of BRIP1/FANCJ (PubMed : 17596542).

See full target information RPA2 phospho T21

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Publications (32)

Recent publications for all applications. Explore the full list and refine your search

The Journal of clinical investigation 135: PubMed40794452

2025

Epigenetic targeting of PGBD5-dependent DNA damage in SMARCB1-deficient sarcomas.

Applications

Unspecified application

Species

Unspecified reactive species

Yaniv Kazansky,Helen S Mueller,Daniel Cameron,Phillip Demarest,Nadia Zaffaroni,Noemi Arrighetti,Valentina Zuco,Prabhjot S Mundi,Yasumichi Kuwahara,Romel Somwar,Rui Qu,Andrea Califano,Elisa de Stanchina,Filemon S Dela Cruz,Andrew L Kung,Mrinal M Gounder,Alex Kentsis

The Journal of biological chemistry 299:105385 PubMed37890780

2023

Atypical genotoxicity of carcinogenic nickel(II): Linkage to dNTP biosynthesis, DNA-incorporated rNMPs, and impaired repair of TOP1-DNA crosslinks.

Applications

Unspecified application

Species

Unspecified reactive species

Casey Krawic,Michal W Luczak,Sophia Valiente,Anatoly Zhitkovich

EMBO molecular medicine 15:e17313 PubMed37491889

2023

ATR inhibition augments the efficacy of lurbinectedin in small-cell lung cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Christopher W Schultz,Yang Zhang,Rajaa Elmeskini,Astrid Zimmermann,Haiqing Fu,Yasuhisa Murai,Darawalee Wangsa,Suresh Kumar,Nobuyuki Takahashi,Devon Atkinson,Liton Kumar Saha,Chien-Fei Lee,Brian Elenbaas,Parth Desai,Robin Sebastian,Ajit Kumar Sharma,Melissa Abel,Brett Schroeder,Manan Krishnamurthy,Rajesh Kumar,Nitin Roper,Mirit Aladjem,Frank T Zenke,Zoe Weaver Ohler,Yves Pommier,Anish Thomas

Nature communications 14:3618 PubMed37336885

2023

ATR kinase supports normal proliferation in the early S phase by preventing replication resource exhaustion.

Applications

Unspecified application

Species

Unspecified reactive species

Demis Menolfi,Brian J Lee,Hanwen Zhang,Wenxia Jiang,Nicole E Bowen,Yunyue Wang,Junfei Zhao,Antony Holmes,Steven Gershik,Raul Rabadan,Baek Kim,Shan Zha

The Journal of cell biology 222: PubMed37036693

2023

RFWD3 promotes ZRANB3 recruitment to regulate the remodeling of stalled replication forks.

Applications

Unspecified application

Species

Unspecified reactive species

Chandler E Moore,Selin E Yalcindag,Hanna Czeladko,Ramya Ravindranathan,Yodhara Wijesekara Hanthi,Juliana C Levy,Vincenzo Sannino,Detlev Schindler,Alberto Ciccia,Vincenzo Costanzo,Andrew E H Elia

Applied biochemistry and biotechnology 195:3477-3490 PubMed36607481

2023

miR-195-5p Targets CDK1 To Regulate New DNA Synthesis and Inhibit the Proliferation of Hepatocellular Carcinoma Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Chunhui Zhou,Sujuan Zhu,Haiping Li

Nature cell biology 23:1095-1104 PubMed34616022

2021

POLθ-mediated end joining is restricted by RAD52 and BRCA2 until the onset of mitosis.

Applications

Unspecified application

Species

Unspecified reactive species

Marta Llorens-Agost,Michael Ensminger,Hang Phuong Le,Anugrah Gawai,Jie Liu,Andrés Cruz-García,Sarita Bhetawal,Richard D Wood,Wolf-Dietrich Heyer,Markus Löbrich

Translational oncology 14:101167 PubMed34280886

2021

MTA2 sensitizes gastric cancer cells to PARP inhibition by induction of DNA replication stress.

Applications

Unspecified application

Species

Unspecified reactive species

Jinwen Shi,Xiaofeng Zhang,Jin'e Li,Wenwen Huang,Yini Wang,Yi Wang,Jun Qin

Molecular cell 81:724-738.e9 PubMed33476576

2021

ER-directed TREX1 limits cGAS activation at micronuclei.

Applications

Unspecified application

Species

Unspecified reactive species

Lisa Mohr,Eléonore Toufektchan,Patrick von Morgen,Kevan Chu,Aakanksha Kapoor,John Maciejowski

Proceedings of the National Academy of Sciences of the United States of America 118: PubMed33431668

2021

ATRX and RECQ5 define distinct homologous recombination subpathways.

Applications

Unspecified application

Species

Unspecified reactive species

Amira Elbakry,Szilvia Juhász,Ki Choi Chan,Markus Löbrich

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-RPA32/RPA2 (phospho T21) antibody [EPR2846(2)]

ELISA - Anti-RPA32/RPA2 (phospho T21) antibody [EPR2846(2)] (AB109394)

Western blot - Anti-RPA32/RPA2 (phospho T21) antibody [EPR2846(2)] (AB109394)

Western blot - Anti-RPA32/RPA2 (phospho T21) antibody [EPR2846(2)] (AB109394)

Western blot - Anti-RPA32/RPA2 (phospho T21) antibody [EPR2846(2)] (AB109394)

Dot Blot - Anti-RPA32/RPA2 (phospho T21) antibody [EPR2846(2)] (AB109394)

Dot Blot - Anti-RPA32/RPA2 (phospho T21) antibody [EPR2846(2)] (AB109394)

Western blot - Anti-RPA32/RPA2 (phospho T21) antibody [EPR2846(2)] (AB109394)

Western blot - Anti-RPA32/RPA2 (phospho T21) antibody [EPR2846(2)] (AB109394)

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Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (32)

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