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Rabbit Recombinant Monoclonal RPA32/RPA2 phospho T21 antibody. Suitable for ELISA, Dot, WB and reacts with Recombinant fragment - Human, Mouse, Rat, Human samples. Cited in 28 publications.


Images

Western blot - Anti-RPA32/RPA2 (phospho T21) antibody [EPR2846(2)] (AB109394), expandable thumbnail
  • ELISA - Anti-RPA32/RPA2 (phospho T21) antibody [EPR2846(2)] (AB109394), expandable thumbnail
  • Dot Blot - Anti-RPA32/RPA2 (phospho T21) antibody [EPR2846(2)] (AB109394), expandable thumbnail
  • Western blot - Anti-RPA32/RPA2 (phospho T21) antibody [EPR2846(2)] (AB109394), expandable thumbnail
  • Western blot - Anti-RPA32/RPA2 (phospho T21) antibody [EPR2846(2)] (AB109394), expandable thumbnail

Publications

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Preservative: 0.05% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 0.1% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
ELISADotWBICC/IFFlow CytIHC-P
Human
Expected
Expected
Tested
Not recommended
Not recommended
Not recommended
Mouse
Expected
Expected
Tested
Not recommended
Not recommended
Not recommended
Rat
Expected
Expected
Tested
Not recommended
Not recommended
Not recommended
Recombinant fragment - Human
Tested
Tested
Not recommended
Not recommended
Not recommended
Not recommended

Tested
Tested

Species

Recombinant fragment - Human

Dilution info

-

Notes

-

Expected
Expected

Species

Mouse, Rat, Human

Dilution info

Use at an assay dependent concentration.

Notes

-

Tested
Tested

Species

Recombinant fragment - Human

Dilution info

1/1000

Notes

-

Expected
Expected

Species

Mouse, Rat, Human

Dilution info

Use at an assay dependent concentration.

Notes

-

Tested
Tested

Species

Mouse

Dilution info

1/50000 - 1/200000

Notes

The antibody has been tested with milk (5%) and BSA (2%) as blocking reagents, but it provided better results with 5%milk.

Species

Rat

Dilution info

1/50000 - 1/200000

Notes

The antibody has been tested with milk (5%) and BSA (2%) as blocking reagents, but it provided better results with 5%milk.

Species

Human

Dilution info

1/50000 - 1/200000

Notes

The antibody has been tested with milk (5%) and BSA (2%) as blocking reagents, but it provided better results with 5%milk.

Not recommended
Not recommended

Species

Recombinant fragment - Human

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Human, Mouse, Rat, Recombinant fragment - Human

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Human, Mouse, Rat, Recombinant fragment - Human

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Human

Dilution info

-

Notes

Heat up to 98 °C, below boiling, and then let cool for 10-20 minutes.

Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.

Species

Mouse

Dilution info

-

Notes

Heat up to 98 °C, below boiling, and then let cool for 10-20 minutes.

Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.

Species

Rat

Dilution info

-

Notes

Heat up to 98 °C, below boiling, and then let cool for 10-20 minutes.

Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.

Species

Recombinant fragment - Human

Dilution info

-

Notes

-

Associated Products

Select an associated product type

15 products for Alternative Product

Target data

Function

As part of the heterotrimeric replication protein A complex (RPA/RP-A), binds and stabilizes single-stranded DNA intermediates that form during DNA replication or upon DNA stress. It prevents their reannealing and in parallel, recruits and activates different proteins and complexes involved in DNA metabolism. Thereby, it plays an essential role both in DNA replication and the cellular response to DNA damage. In the cellular response to DNA damage, the RPA complex controls DNA repair and DNA damage checkpoint activation. Through recruitment of ATRIP activates the ATR kinase a master regulator of the DNA damage response. It is required for the recruitment of the DNA double-strand break repair factors RAD51 and RAD52 to chromatin in response to DNA damage. Also recruits to sites of DNA damage proteins like XPA and XPG that are involved in nucleotide excision repair and is required for this mechanism of DNA repair. Also plays a role in base excision repair (BER) probably through interaction with UNG. Also recruits SMARCAL1/HARP, which is involved in replication fork restart, to sites of DNA damage. May also play a role in telomere maintenance. RPA stimulates 5'-3' helicase activity of BRIP1/FANCJ (PubMed:17596542).

Alternative names

Recommended products

Rabbit Recombinant Monoclonal RPA32/RPA2 phospho T21 antibody. Suitable for ELISA, Dot, WB and reacts with Recombinant fragment - Human, Mouse, Rat, Human samples. Cited in 28 publications.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

EPR2846(2)

Purification technique

Affinity purification Protein A

Specificity

ab109394 only detects RPA32/RPA2 phosphorylated at threonine 21. The antibody has been tested with milk (5%) and BSA (2%) as blocking reagents, but it provided better results with 5% milk.

This antibody cross-reactivates with non-phospho substrate in specific situations.

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Stable for 12 months at -20°C

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

8 product images

  • Western blot - Anti-RPA32/RPA2 (phospho T21) antibody [EPR2846(2)] (ab109394), expandable thumbnail

    Western blot - Anti-RPA32/RPA2 (phospho T21) antibody [EPR2846(2)] (ab109394)

    Blocking/Diluting buffer and concentration: 5% NFDM/TBST

    All lanes: Western blot - Anti-RPA32/RPA2 (phospho T21) antibody [EPR2846(2)] (ab109394) at 1/5000 dilution

    Lane 1: MDA-MB-231 (Human breast adenocarcinoma epithelial cell) whole cell lysates at 15 µg

    Lane 2: MDA-MB-231 (Human breast adenocarcinoma epithelial cell) whole cell lysates. Then the membrane was incubated with phosphatase. at 15 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Predicted band size: 29 kDa

    Observed band size: 32 kDa, 36 kDa

    Exposure time: 30s

  • ELISA - Anti-RPA32/RPA2 (phospho T21) antibody [EPR2846(2)] (ab109394), expandable thumbnail

    ELISA - Anti-RPA32/RPA2 (phospho T21) antibody [EPR2846(2)] (ab109394)

    Direct ELISA antigen dose-response curve using purified ab109394.

    Primary antibody: ab109394 (0-1000nweg/ml).

    Antigens: P1: Human RPA32/RPA2 (phospho T21) at 10ng/ml and 1ng/ml; NP: non-phospho at 10ng/ml and 1 ng/ml.

    Secondary antibody: Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at 1/2500 dilution.

  • Dot Blot - Anti-RPA32/RPA2 (phospho T21) antibody [EPR2846(2)] (ab109394), expandable thumbnail

    Dot Blot - Anti-RPA32/RPA2 (phospho T21) antibody [EPR2846(2)] (ab109394)

    Dot blot analysis of human c-Myb RPA32/RPA2 (pT21) phospho peptide (Lane 1) and RPA32/RPA2 non-phospho peptide (Lane 2) labeling RPA32/RPA2 (phospho T21) with purified ab109394 at a dilution of 1/5000. Goat Anti-Rabbit IgG H&L (HRP) ab97051 (Peroxidase conjugated goat anti-rabbit IgG (H+L)) was used as the secondary antibody at a dilution of 1/2500.

    Blocking and dilution buffer: 5% NFDM/TBST.

    Exposure time: 3 minutes.

  • Western blot - Anti-RPA32/RPA2 (phospho T21) antibody [EPR2846(2)] (ab109394), expandable thumbnail

    Western blot - Anti-RPA32/RPA2 (phospho T21) antibody [EPR2846(2)] (ab109394)

    Blocking/Diluting buffer and concentration:5% NFDM/TBST.

    Exposure time: 110 seconds.

    All lanes: Western blot - Anti-RPA32/RPA2 (phospho T21) antibody [EPR2846(2)] (ab109394) at 1/2000 dilution

    Lane 1: C6 (Rat glial tumor glial cell) whole cell lysates at 15 µg

    Lane 2: C6 (Rat glial tumor glial cell) treated with 100nM calyculin A for 60 minutes whole cell lysates at 15 µg

    Lane 3: C6 (Rat glial tumor glial cell) treated with 100nM calyculin A for 60 minutes whole cell lysates.Then the membrane was incubated with phosphatase. at 15 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Predicted band size: 29 kDa

    Observed band size: 32 kDa, 36 kDa

  • Western blot - Anti-RPA32/RPA2 (phospho T21) antibody [EPR2846(2)] (ab109394), expandable thumbnail

    Western blot - Anti-RPA32/RPA2 (phospho T21) antibody [EPR2846(2)] (ab109394)

    Blocking/Diluting buffer and concentration: 5% NFDM/TBST.

    Exposure time: 180 seconds.

    All lanes: Western blot - Anti-RPA32/RPA2 (phospho T21) antibody [EPR2846(2)] (ab109394) at 1/2000 dilution

    Lane 1: NIH/3T3 (Mouse embryonic fibroblast) whole cell lysates at 15 µg

    Lane 2: NIH/3T3 (Mouse embryonic fibroblast) treated with 100ng/ml calyculin A for 30 minutes whole cell lysates at 15 µg

    Lane 3: NIH/3T3 (Mouse embryonic fibroblast) treated with 100ng/ml calyculin A for 60 3minutes whole cell lysates.Then the membrane was incubated with phosphatase at 15 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Predicted band size: 29 kDa

    Observed band size: 32 kDa, 36 kDa

  • Western blot - Anti-RPA32/RPA2 (phospho T21) antibody [EPR2846(2)] (ab109394), expandable thumbnail

    Western blot - Anti-RPA32/RPA2 (phospho T21) antibody [EPR2846(2)] (ab109394)

    Blocking and dilution buffer: 5% NFDM/TBST.

    All lanes: Western blot - Anti-RPA32/RPA2 (phospho T21) antibody [EPR2846(2)] (ab109394) at 1/50000 dilution

    Lane 1: HeLa whole cell lysate - untreated at 10 µg

    Lane 2: HeLa whole cell lysate - treated with Calyculin A at 10 µg

    Lane 3: HeLa whole cell lysate - treated with Calyculin A and Alkaline Phosphatase at 10 µg

    Secondary

    All lanes: Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

    Predicted band size: 29 kDa

    Observed band size: 32 kDa

    Exposure time: 30s

  • Dot Blot - Anti-RPA32/RPA2 (phospho T21) antibody [EPR2846(2)] (ab109394), expandable thumbnail

    Dot Blot - Anti-RPA32/RPA2 (phospho T21) antibody [EPR2846(2)] (ab109394)

    Dot blot analysis of RPA32/RPA2 (pT21) phospho peptide (lane 1) and RPA32/RPA2 non-phospho peptide (lane 2) labelling RPA32/RPA2 (phospho T21) with ab109394 at a dilution of 1/1000. A peroxidase-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/2500).

    Blocking and dilution buffer: 5% NFDM/TBST.

    Exposure time: 3 minutes.

  • Western blot - Anti-RPA32/RPA2 (phospho T21) antibody [EPR2846(2)] (ab109394), expandable thumbnail

    Western blot - Anti-RPA32/RPA2 (phospho T21) antibody [EPR2846(2)] (ab109394)

    All lanes: Western blot - Anti-RPA32/RPA2 (phospho T21) antibody [EPR2846(2)] (ab109394) at 1/50000 dilution

    Lane 1: HeLa cell lysates, untreated at 10 µg

    Lane 2: HeLa cell lysates, treated with Camptothecin at 10 µg

    Predicted band size: 29 kDa

    Observed band size: 32 kDa

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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