Rabbit Recombinant Monoclonal RPA32/RPA2 phospho T21 antibody. Suitable for ELISA, Dot, WB and reacts with Recombinant fragment - Human, Mouse, Rat, Human samples. Cited in 28 publications.
IgG
Rabbit
pH: 7.2 - 7.4
Preservative: 0.05% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 0.1% BSA
Liquid
Monoclonal
ELISA | Dot | WB | ICC/IF | Flow Cyt | IHC-P | |
---|---|---|---|---|---|---|
Human | Expected | Expected | Tested | Not recommended | Not recommended | Not recommended |
Mouse | Expected | Expected | Tested | Not recommended | Not recommended | Not recommended |
Rat | Expected | Expected | Tested | Not recommended | Not recommended | Not recommended |
Recombinant fragment - Human | Tested | Tested | Not recommended | Not recommended | Not recommended | Not recommended |
Species | Dilution info | Notes |
---|---|---|
Species Recombinant fragment - Human | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse, Rat, Human | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Recombinant fragment - Human | Dilution info 1/1000 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse, Rat, Human | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info 1/50000 - 1/200000 | Notes The antibody has been tested with milk (5%) and BSA (2%) as blocking reagents, but it provided better results with 5%milk. |
Species Rat | Dilution info 1/50000 - 1/200000 | Notes The antibody has been tested with milk (5%) and BSA (2%) as blocking reagents, but it provided better results with 5%milk. |
Species Human | Dilution info 1/50000 - 1/200000 | Notes The antibody has been tested with milk (5%) and BSA (2%) as blocking reagents, but it provided better results with 5%milk. |
Species | Dilution info | Notes |
---|---|---|
Species Recombinant fragment - Human | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human, Mouse, Rat, Recombinant fragment - Human | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human, Mouse, Rat, Recombinant fragment - Human | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info - | Notes Heat up to 98 °C, below boiling, and then let cool for 10-20 minutes. Perform heat-mediated antigen retrieval before commencing with IHC staining protocol. |
Species Mouse | Dilution info - | Notes Heat up to 98 °C, below boiling, and then let cool for 10-20 minutes. Perform heat-mediated antigen retrieval before commencing with IHC staining protocol. |
Species Rat | Dilution info - | Notes Heat up to 98 °C, below boiling, and then let cool for 10-20 minutes. Perform heat-mediated antigen retrieval before commencing with IHC staining protocol. |
Species Recombinant fragment - Human | Dilution info - | Notes - |
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As part of the heterotrimeric replication protein A complex (RPA/RP-A), binds and stabilizes single-stranded DNA intermediates that form during DNA replication or upon DNA stress. It prevents their reannealing and in parallel, recruits and activates different proteins and complexes involved in DNA metabolism. Thereby, it plays an essential role both in DNA replication and the cellular response to DNA damage. In the cellular response to DNA damage, the RPA complex controls DNA repair and DNA damage checkpoint activation. Through recruitment of ATRIP activates the ATR kinase a master regulator of the DNA damage response. It is required for the recruitment of the DNA double-strand break repair factors RAD51 and RAD52 to chromatin in response to DNA damage. Also recruits to sites of DNA damage proteins like XPA and XPG that are involved in nucleotide excision repair and is required for this mechanism of DNA repair. Also plays a role in base excision repair (BER) probably through interaction with UNG. Also recruits SMARCAL1/HARP, which is involved in replication fork restart, to sites of DNA damage. May also play a role in telomere maintenance. RPA stimulates 5'-3' helicase activity of BRIP1/FANCJ (PubMed:17596542).
REPA2, RPA32, RPA34, RPA2, Replication protein A 32 kDa subunit, RP-A p32, Replication factor A protein 2, Replication protein A 34 kDa subunit, RF-A protein 2, RP-A p34
Rabbit Recombinant Monoclonal RPA32/RPA2 phospho T21 antibody. Suitable for ELISA, Dot, WB and reacts with Recombinant fragment - Human, Mouse, Rat, Human samples. Cited in 28 publications.
IgG
Rabbit
pH: 7.2 - 7.4
Preservative: 0.05% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 0.1% BSA
Liquid
Monoclonal
EPR2846(2)
Affinity purification Protein A
ab109394 only detects RPA32/RPA2 phosphorylated at threonine 21. The antibody has been tested with milk (5%) and BSA (2%) as blocking reagents, but it provided better results with 5% milk.
This antibody cross-reactivates with non-phospho substrate in specific situations.
Blue Ice
1-2 weeks
+4°C
-20°C
Upon delivery aliquot
Stable for 12 months at -20°C
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
For more information, read more on recombinant antibodies.
We have tested this species and application combination and it works. It is covered by our product promise.
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
Blocking/Diluting buffer and concentration: 5% NFDM/TBST
All lanes: Western blot - Anti-RPA32/RPA2 (phospho T21) antibody [EPR2846(2)] (ab109394) at 1/5000 dilution
Lane 1: MDA-MB-231 (Human breast adenocarcinoma epithelial cell) whole cell lysates at 15 µg
Lane 2: MDA-MB-231 (Human breast adenocarcinoma epithelial cell) whole cell lysates. Then the membrane was incubated with phosphatase. at 15 µg
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Predicted band size: 29 kDa
Observed band size: 32 kDa, 36 kDa
Exposure time: 30s
Direct ELISA antigen dose-response curve using purified ab109394.
Primary antibody: ab109394 (0-1000nweg/ml).
Antigens: P1: Human RPA32/RPA2 (phospho T21) at 10ng/ml and 1ng/ml; NP: non-phospho at 10ng/ml and 1 ng/ml.
Secondary antibody: Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at 1/2500 dilution.
Dot blot analysis of human c-Myb RPA32/RPA2 (pT21) phospho peptide (Lane 1) and RPA32/RPA2 non-phospho peptide (Lane 2) labeling RPA32/RPA2 (phospho T21) with purified ab109394 at a dilution of 1/5000. Goat Anti-Rabbit IgG H&L (HRP) ab97051 (Peroxidase conjugated goat anti-rabbit IgG (H+L)) was used as the secondary antibody at a dilution of 1/2500.
Blocking and dilution buffer: 5% NFDM/TBST.
Exposure time: 3 minutes.
Blocking/Diluting buffer and concentration:5% NFDM/TBST.
Exposure time: 110 seconds.
All lanes: Western blot - Anti-RPA32/RPA2 (phospho T21) antibody [EPR2846(2)] (ab109394) at 1/2000 dilution
Lane 1: C6 (Rat glial tumor glial cell) whole cell lysates at 15 µg
Lane 2: C6 (Rat glial tumor glial cell) treated with 100nM calyculin A for 60 minutes whole cell lysates at 15 µg
Lane 3: C6 (Rat glial tumor glial cell) treated with 100nM calyculin A for 60 minutes whole cell lysates.Then the membrane was incubated with phosphatase. at 15 µg
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Predicted band size: 29 kDa
Observed band size: 32 kDa, 36 kDa
Blocking/Diluting buffer and concentration: 5% NFDM/TBST.
Exposure time: 180 seconds.
All lanes: Western blot - Anti-RPA32/RPA2 (phospho T21) antibody [EPR2846(2)] (ab109394) at 1/2000 dilution
Lane 1: NIH/3T3 (Mouse embryonic fibroblast) whole cell lysates at 15 µg
Lane 2: NIH/3T3 (Mouse embryonic fibroblast) treated with 100ng/ml calyculin A for 30 minutes whole cell lysates at 15 µg
Lane 3: NIH/3T3 (Mouse embryonic fibroblast) treated with 100ng/ml calyculin A for 60 3minutes whole cell lysates.Then the membrane was incubated with phosphatase at 15 µg
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Predicted band size: 29 kDa
Observed band size: 32 kDa, 36 kDa
Blocking and dilution buffer: 5% NFDM/TBST.
All lanes: Western blot - Anti-RPA32/RPA2 (phospho T21) antibody [EPR2846(2)] (ab109394) at 1/50000 dilution
Lane 1: HeLa whole cell lysate - untreated at 10 µg
Lane 2: HeLa whole cell lysate - treated with Calyculin A at 10 µg
Lane 3: HeLa whole cell lysate - treated with Calyculin A and Alkaline Phosphatase at 10 µg
All lanes: Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution
Predicted band size: 29 kDa
Observed band size: 32 kDa
Exposure time: 30s
Dot blot analysis of RPA32/RPA2 (pT21) phospho peptide (lane 1) and RPA32/RPA2 non-phospho peptide (lane 2) labelling RPA32/RPA2 (phospho T21) with ab109394 at a dilution of 1/1000. A peroxidase-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/2500).
Blocking and dilution buffer: 5% NFDM/TBST.
Exposure time: 3 minutes.
All lanes: Western blot - Anti-RPA32/RPA2 (phospho T21) antibody [EPR2846(2)] (ab109394) at 1/50000 dilution
Lane 1: HeLa cell lysates, untreated at 10 µg
Lane 2: HeLa cell lysates, treated with Camptothecin at 10 µg
Predicted band size: 29 kDa
Observed band size: 32 kDa
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