AB240943

Anti-RPA32/RPA2 (phospho T21) antibody [EPR2846(2)] - BSA and Azide free

RabMAb
Recombinant
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(1 Publication)

Rabbit Recombinant Monoclonal RPA32/RPA2 phospho T21 antibody. Carrier free. Suitable for ELISA, Dot, WB and reacts with Synthetic peptide - Human, Human, Rat, Mouse samples. Cited in 1 publication.

View Alternative Names

REPA2, RPA32, RPA34, RPA2, Replication protein A 32 kDa subunit, RP-A p32, Replication factor A protein 2, Replication protein A 34 kDa subunit, RF-A protein 2, RP-A p34

4 Images

ELISA - Anti-RPA32/RPA2 (phospho T21) antibody [EPR2846(2)] - BSA and Azide free (AB240943)

ELISA

Supplier Data

ELISA - Anti-RPA32/RPA2 (phospho T21) antibody [EPR2846(2)] - BSA and Azide free (AB240943)

Direct ELISA antigen dose-response curve using purified ab109394.

Primary antibody : ab109394 (0-1000nweg/ml).

Antigens : P1 : Human RPA32/RPA2 (phospho T21) at 10ng/ml and 1ng/ml; NP : non-phospho at 10ng/ml and 1 ng/ml.

Secondary antibody : Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at 1/2500 dilution.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab109394).

Lab

Western blot - Anti-RPA32/RPA2 (phospho T21) antibody [EPR2846(2)] - BSA and Azide free (AB240943)

Blocking and dilution buffer : 5% NFDM/TBST.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab109394).

All lanes:

Western blot - Anti-RPA32/RPA2 (phospho T21) antibody [EPR2846(2)] (<a href='/en-us/products/primary-antibodies/rpa32-rpa2-phospho-t21-antibody-epr28462-ab109394'>ab109394</a>) at 1/50000 dilution

Lane 1:

HeLa whole cell lysate - untreated at 10 µg

Lane 2:

HeLa whole cell lysate - treated with Calyculin A at 10 µg

Lane 3:

HeLa whole cell lysate - treated with Calyculin A and Alkaline Phosphatase at 10 µg

Secondary

All lanes:

Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

Predicted band size: 29 kDa

Observed band size: 32 kDa

false

Exposure time: 30s

Supplier Data

Dot Blot - Anti-RPA32/RPA2 (phospho T21) antibody [EPR2846(2)] - BSA and Azide free (AB240943)

Dot blot analysis of human c-Myb RPA32/RPA2 (pT21) phospho peptide (Lane 1) and human RPA32/RPA2 non-phospho peptide (Lane 2) labeling RPA32/RPA2 (phospho T21) with purified ab109394 at a dilution of 1/5000. ab97051 (Peroxidase conjugated goat anti-rabbit IgG (H+L)) was used as the secondary antibody at a dilution of 1/2500.

Blocking and dilution buffer : 5% NFDM/TBST.

Exposure time : 3 minutes.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab109394).

Lab

Dot Blot - Anti-RPA32/RPA2 (phospho T21) antibody [EPR2846(2)] - BSA and Azide free (AB240943)

Dot blot analysis of human RPA32/RPA2 (pT21) phospho peptide (lane 1) and human RPA32/RPA2 non-phospho peptide (lane 2) labelling RPA32/RPA2 (phospho T21) with ab109394 at a dilution of 1/1000. A peroxidase-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/2500).

Blocking and dilution buffer : 5% NFDM/TBST.

Exposure time : 3 minutes.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab109394).

Unconjugated

Anti-RPA32/RPA2 (phospho T21) antibody [EPR2846(2)]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR2846(2)

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Rat, Human

Applications

ELISA, WB, Dot

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

ab109394 only detects RPA32/RPA2 phosphorylated at threonine 21. The antibody has been tested with milk (5%) and BSA (2%) as blocking reagents, but it provided better results with 5% milk. This antibody cross-reactivates with non-phospho substrate in specific situations.

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Reactivity data

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Product details

ab240943 is the carrier-free version of ab109394.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

As part of the heterotrimeric replication protein A complex (RPA/RP-A), binds and stabilizes single-stranded DNA intermediates that form during DNA replication or upon DNA stress. It prevents their reannealing and in parallel, recruits and activates different proteins and complexes involved in DNA metabolism. Thereby, it plays an essential role both in DNA replication and the cellular response to DNA damage. In the cellular response to DNA damage, the RPA complex controls DNA repair and DNA damage checkpoint activation. Through recruitment of ATRIP activates the ATR kinase a master regulator of the DNA damage response. It is required for the recruitment of the DNA double-strand break repair factors RAD51 and RAD52 to chromatin in response to DNA damage. Also recruits to sites of DNA damage proteins like XPA and XPG that are involved in nucleotide excision repair and is required for this mechanism of DNA repair. Also plays a role in base excision repair (BER) probably through interaction with UNG. Also recruits SMARCAL1/HARP, which is involved in replication fork restart, to sites of DNA damage. May also play a role in telomere maintenance. RPA stimulates 5'-3' helicase activity of BRIP1/FANCJ (PubMed : 17596542).

See full target information RPA2 pT21

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Oncology letters 21:486 PubMed33968202

2021

Circular RNA UBAP2 promotes the proliferation of prostate cancer cells via the miR-1244/MAP3K2 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaodong Li,Baihetiya Azhati,Wenguang Wang,Mulati Rexiati,Chen Xing,Yujie Wang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-RPA32/RPA2 (phospho T21) antibody [EPR2846(2)] - BSA and Azide free

ELISA - Anti-RPA32/RPA2 (phospho T21) antibody [EPR2846(2)] - BSA and Azide free (AB240943)

Western blot - Anti-RPA32/RPA2 (phospho T21) antibody [EPR2846(2)] - BSA and Azide free (AB240943)

Dot Blot - Anti-RPA32/RPA2 (phospho T21) antibody [EPR2846(2)] - BSA and Azide free (AB240943)

Dot Blot - Anti-RPA32/RPA2 (phospho T21) antibody [EPR2846(2)] - BSA and Azide free (AB240943)

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Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Product protocols

Target data

Publications (1)

Product promise