Rabbit Recombinant Monoclonal RPA70 antibody. Carrier free. Suitable for IHC-P, WB and reacts with Mouse, Rat samples.
Images
![Western blot - Anti-RPA70 antibody [EPR27138-43] - BSA and Azide free (AB317737), expandable thumbnail](https://content.abcam.com/products/images/rpa70-antibody-epr27138-43-bsa-and-azide-free-ab317737--western-blot-img432564.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-RPA70 antibody [EPR27138-43] - BSA and Azide free (AB317737)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPA70 antibody [EPR27138-43] - BSA and Azide free (AB317737), expandable thumbnail](https://content.abcam.com/products/images/rpa70-antibody-epr27138-43-bsa-and-azide-free-ab317737--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img432607.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPA70 antibody [EPR27138-43] - BSA and Azide free (AB317737)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPA70 antibody [EPR27138-43] - BSA and Azide free (AB317737), expandable thumbnail](https://content.abcam.com/products/images/rpa70-antibody-epr27138-43-bsa-and-azide-free-ab317737--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img432608.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPA70 antibody [EPR27138-43] - BSA and Azide free (AB317737)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPA70 antibody [EPR27138-43] - BSA and Azide free (AB317737), expandable thumbnail](https://content.abcam.com/products/images/rpa70-antibody-epr27138-43-bsa-and-azide-free-ab317737--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img432609.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPA70 antibody [EPR27138-43] - BSA and Azide free (AB317737)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IHC-P | WB | IP | |
|---|---|---|---|
| Mouse | Tested | Tested | Not recommended |
| Rat | Tested | Tested | Not recommended |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Rat | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Target data
Function
As part of the heterotrimeric replication protein A complex (RPA/RP-A), binds and stabilizes single-stranded DNA intermediates, that form during DNA replication or upon DNA stress. It prevents their reannealing and in parallel, recruits and activates different proteins and complexes involved in DNA metabolism. Thereby, it plays an essential role both in DNA replication and the cellular response to DNA damage. In the cellular response to DNA damage, the RPA complex controls DNA repair and DNA damage checkpoint activation. Through recruitment of ATRIP activates the ATR kinase a master regulator of the DNA damage response. It is required for the recruitment of the DNA double-strand break repair factors RAD51 and RAD52 to chromatin in response to DNA damage. Also recruits to sites of DNA damage proteins like XPA and XPG that are involved in nucleotide excision repair and is required for this mechanism of DNA repair. Also plays a role in base excision repair (BER) probably through interaction with UNG. Also recruits SMARCAL1/HARP, which is involved in replication fork restart, to sites of DNA damage. May also play a role in telomere maintenance.
Alternative names
Replication protein A 70 kDa DNA-binding subunit, RP-A p70, Replication factor A protein 1, RF-A protein 1
Recommended products
Rabbit Recombinant Monoclonal RPA70 antibody. Carrier free. Suitable for IHC-P, WB and reacts with Mouse, Rat samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR27138-43
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
Notes
ab317737 is the carrier-free version of Anti-RPA70 antibody [EPR27138-43] ab317736.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
4 product images
![Western blot - Anti-RPA70 antibody [EPR27138-43] - BSA and Azide free (ab317737), expandable thumbnail](https://content.abcam.com/products/images/rpa70-antibody-epr27138-43-bsa-and-azide-free-ab317737--western-blot-img432564.jpg "Western blot - Anti-RPA70 antibody [EPR27138-43] - BSA and Azide free (ab317737)")
Western blot - Anti-RPA70 antibody [EPR27138-43] - BSA and Azide free (ab317737)
This data was developed using Anti-RPA70 antibody [EPR27138-43] ab317736, the same antibody clone in a different buffer formulation.
The molecular weight observed is consistent with what has been described in the literature (PMID: 8611550, PMID: 12600993). The ~52 kDa band likely represents a C-terminal cleavage product of RPA70.
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.
Exposure time: Lanes 1-2: 10 seconds; Lanes 3: 59 seconds.
All lanes: Western blot - Anti-RPA70 antibody [EPR27138-43] (Anti-RPA70 antibody [EPR27138-43] ab317736) at 1/1000 dilution
Lane 1: NIH/3T3 (mouse embryonic fibroblast) transfected with scrambled siRNA control whole cell lysate at 20 µg with NFDM/TBST
Lane 2: NIH/3T3 transfected with siRNA specifically targeting RPA70 whole cell lysate at 20 µg with NFDM/TBST
Lane 3: Neuro-2a (mouse neuroblastoma neuroblast) whole cell lysate at 20 µg with NFDM/TBST
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Observed band size: 70 kDa, 52 kDa, 36 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPA70 antibody [EPR27138-43] - BSA and Azide free (ab317737), expandable thumbnail](https://content.abcam.com/products/images/rpa70-antibody-epr27138-43-bsa-and-azide-free-ab317737--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img432607.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPA70 antibody [EPR27138-43] - BSA and Azide free (ab317737)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPA70 antibody [EPR27138-43] - BSA and Azide free (ab317737)
This data was developed using Anti-RPA70 antibody [EPR27138-43] ab317736, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Mouse spleen tissue labeling RPA70 with Anti-RPA70 antibody [EPR27138-43] ab317736 at 1/5000 (0.102 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining in mouse spleen.
The section was incubated with Anti-RPA70 antibody [EPR27138-43] ab317736 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrumentCounterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPA70 antibody [EPR27138-43] - BSA and Azide free (ab317737), expandable thumbnail](https://content.abcam.com/products/images/rpa70-antibody-epr27138-43-bsa-and-azide-free-ab317737--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img432608.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPA70 antibody [EPR27138-43] - BSA and Azide free (ab317737)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPA70 antibody [EPR27138-43] - BSA and Azide free (ab317737)
This data was developed using Anti-RPA70 antibody [EPR27138-43] ab317736, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Rat spleen tissue labeling RPA70 with Anti-RPA70 antibody [EPR27138-43] ab317736 at 1/5000 (0.102 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining in rat spleen.
The section was incubated with Anti-RPA70 antibody [EPR27138-43] ab317736 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrumentCounterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPA70 antibody [EPR27138-43] - BSA and Azide free (ab317737), expandable thumbnail](https://content.abcam.com/products/images/rpa70-antibody-epr27138-43-bsa-and-azide-free-ab317737--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img432609.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPA70 antibody [EPR27138-43] - BSA and Azide free (ab317737)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPA70 antibody [EPR27138-43] - BSA and Azide free (ab317737)
This data was developed using Anti-RPA70 antibody [EPR27138-43] ab317736, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Mouse Burkitt's lymphoma tissue labeling RPA70 with Anti-RPA70 antibody [EPR27138-43] ab317736 at 1/5000 (0.102 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining in mouse Burkitt's lymphoma.
The section was incubated with Anti-RPA70 antibody [EPR27138-43] ab317736 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrumentCounterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
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