Anti-RPA70 antibody [EPR3472] - BSA and Azide free

7 product images

• 

  Immunocytochemistry/ Immunofluorescence - Anti-RPA70 antibody [EPR3472] - BSA and Azide free (ab239890)

  Immunocytochemistry/Immunofluorescence analysis of A549 cells labelling RPA70 with purified Anti-RPA70 antibody [EPR3472] ab79398 at 1/200. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077, an Alexa Fluor^® 488-conjugated goat anti-rabbit IgG (1/500) was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain. Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291, a mouse anti-tubulin (1/1000) and Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120, an Alexa Fluor^® 594-conjugated goat anti-mouse IgG (1/500) were also used.

  Control 1: primary antibody (1/200) and secondary antibody, Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120, an Alexa Fluor^® 594-conjugated goat anti-mouse IgG (1/500).

  Control 2: Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 (1/1000) and secondary antibody, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077, an Alexa Fluor^® 488-conjugated goat anti-rabbit IgG (1/500).

  This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-RPA70 antibody [EPR3472] ab79398).

• 

  Immunoprecipitation - Anti-RPA70 antibody [EPR3472] - BSA and Azide free (ab239890)

  Anti-RPA70 antibody [EPR3472] ab79398 (purified) at 1/20 immunoprecipitating RPA70 in HeLa whole cell lysate. 

  Lane 1 (input): HeLa whole cell lysate (10µg)

  Lane 2 (+): Anti-RPA70 antibody [EPR3472] ab79398 + HeLa whole cell lysate (10µg).

  Lane 3 (-): Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-RPA70 antibody [EPR3472] ab79398 in HeLa whole cell lysate.

  For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the secondary antibody (1/1500).

  Blocking buffer and concentration: 5% NFDM/TBST.

  Diluting buffer and concentration: 5% NFDM /TBST.

  This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-RPA70 antibody [EPR3472] ab79398).

  All lanes: Immunoprecipitation - Anti-RPA70 antibody [EPR3472] (Anti-RPA70 antibody [EPR3472] ab79398)

  Predicted band size: 68 kDa

  Observed band size: 70 kDa

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPA70 antibody [EPR3472] - BSA and Azide free (ab239890)

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human cervix carcinoma tissue labelling RPA70 with purified Anti-RPA70 antibody [EPR3472] ab79398 at 1/100. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. Goat Anti-Rabbit IgG H&L (HRP) ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

  This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-RPA70 antibody [EPR3472] ab79398).

• 

  Flow Cytometry (Intracellular) - Anti-RPA70 antibody [EPR3472] - BSA and Azide free (ab239890)

  Intracellular Flow Cytometry analysis of HeLa cells labelling RPA70 with purified Anti-RPA70 antibody [EPR3472] ab79398 at 1/80 (red). Cells were fixed with 2% paraformaldehyde. A FITC-conjugated goat anti-rabbit IgG (1/150) was used as the secondary antibody. Black - Isotype control, rabbit monoclonal IgG. Blue - Unlabelled control, cells without incubation with primary and secondary antibodies.
  

  This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-RPA70 antibody [EPR3472] ab79398).

• 

  This image is courtesy of a customer review submitted by Remi Buisson.

  Immunocytochemistry/ Immunofluorescence - Anti-RPA70 antibody [EPR3472] - BSA and Azide free (ab239890)

  Unpurified Anti-RPA70 antibody [EPR3472] ab79398 staining RPA70 in U2OS cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.5% Triton X-100 in PBS and blocked with 2% BSA for 1 hour at 25°C. Samples were incubated with primary antibody (1/500 in PBS + 0.5% Tween-20) for 2 hours at 25°C. A Cy3^®-conjugated goat anti-rabbit IgG monoclonal (1/250) was used as the secondary antibody.

  This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-RPA70 antibody [EPR3472] ab79398).

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPA70 antibody [EPR3472] - BSA and Azide free (ab239890)

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human cervical squamous cell carcinoma labelling RPA70 with unpurified Anti-RPA70 antibody [EPR3472] ab79398 at a dilution of 1/100.
  

  This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-RPA70 antibody [EPR3472] ab79398).

  Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

• 

  Flow Cytometry (Intracellular) - Anti-RPA70 antibody [EPR3472] - BSA and Azide free (ab239890)

  Overlay histogram showing HeLa cells stained with unpurified Anti-RPA70 antibody [EPR3472] ab79398 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (unpurified Anti-RPA70 antibody [EPR3472] ab79398, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was DyLight^® 488 goat anti-rabbit IgG (H+L) (Goat Anti-Rabbit IgG H&L (DyLight® 488) preadsorbed ab96899) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1μg/1x10⁶ cells) used under the same conditions. Acquisition of >5,000 events was performed.
  

  This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-RPA70 antibody [EPR3472] ab79398).