JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB138436

Anti-RPB3 antibody

Be the first to review this product! Submit a review

|

(2 Publications)

Rabbit Polyclonal RPB3 antibody. Suitable for IHC-P, IP, WB and reacts with Mouse, Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human POLR2C aa 200 to C-terminus.

View Alternative Names

A-152E5.7, POLR2C, DNA-directed RNA polymerase II subunit RPB3, RNA polymerase II subunit 3, RNA polymerase II subunit B3, DNA-directed RNA polymerase II 33 kDa polypeptide, DNA-directed RNA polymerase II subunit C, RPB31, RPB33

4 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPB3 antibody (AB138436)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPB3 antibody (AB138436)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded human breast carcinoma tissue labelling RPB3 with ab138436 at a 1/1000 dilution.

Immunoprecipitation - Anti-RPB3 antibody (AB138436)
  • IP

Unknown

Immunoprecipitation - Anti-RPB3 antibody (AB138436)

Immunoprecipitation detection of RPB3 in HeLa whole cell lysate (1 mg/IP; 20% of IP loaded/lane) using ab138436 at 6μg/mg lysate. Subsequent Western blot detection step was performed using ab138436 at 1μg/ml. Chemiluminescence with an exposure time of 10 seconds.

All lanes:

Immunoprecipitation - Anti-RPB3 antibody (ab138436)

Predicted band size: 14 kDa,31 kDa,57 kDa

Observed band size: 14 kDa,57 kDa

false

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPB3 antibody (AB138436)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPB3 antibody (AB138436)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded mouse plasmacytoma tissue labelling RPB3 with ab138436 at a 1/1000 dilution.

Western blot - Anti-RPB3 antibody (AB138436)
  • WB

Unknown

Western blot - Anti-RPB3 antibody (AB138436)

All lanes:

Western blot - Anti-RPB3 antibody (ab138436) at 1/2000 dilution

Lane 1:

HeLa cell lysate at 50 µg

Lane 2:

HeLa cell lysate at 15 µg

Lane 3:

293T cell lysate at 50 µg

Lane 4:

Jurkat cell lysate at 50 µg

Predicted band size: 31 kDa

true

Exposure time: 3min

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

IHC-P, IP, WB

applications

Immunogen

Synthetic Peptide within Human POLR2C aa 200 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P19387

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/500 - 1/2000", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-10 µg/mg of lysate", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/500 - 1/2000", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chicken": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chimpanzee": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Dog": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Gorilla": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Guinea pig": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Horse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Pig": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rhesus monkey": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Xenopus laevis": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Xenopus tropicalis": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Zebrafish": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
style
left-column
style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: 99.01% Tris citrate/phosphate
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
style
left-column
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Core component of RNA polymerase II (Pol II), a DNA-dependent RNA polymerase which synthesizes mRNA precursors and many functional non-coding RNAs using the four ribonucleoside triphosphates as substrates.
See full target information POLR2C
style
left-column

Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Cell discovery 4:4 PubMed29423272

2018

Loss of ASXL1 in the bone marrow niche dysregulates hematopoietic stem and progenitor cell fates.

Applications

Unspecified application

Species

Unspecified reactive species

Peng Zhang,Zizhen Chen,Rong Li,Ying Guo,Hui Shi,Jie Bai,Hui Yang,Mengyao Sheng,Zhaomin Li,Zhuo Li,Jianping Li,Shi Chen,Weiping Yuan,Tao Cheng,Mingjiang Xu,Yuan Zhou,Feng-Chun Yang

NPJ breast cancer 2: PubMed27583302

2016

A microchip platform for structural oncology applications.

Applications

IP

Species

Unspecified reactive species

Carly E Winton,Brian L Gilmore,Andrew C Demmert,Vasilea Karageorge,Zhi Sheng,Deborah F Kelly
View all publications
style
left-column
style
left-column
style
right-column

Alternative Products

Primary Antibodies

AB202893

Anti-RPB3 antibody [1Y26]

Western blot - Anti-RPB3 antibody [1Y26] (AB202893)

  • 0
  • 0
Primary Antibodies

AB181193

Anti-RPB3 antibody [EPR13300(B)] - C-terminal

RabMAb|Recombinant

Western blot - Anti-RPB3 antibody [EPR13300(B)] - C-terminal (AB181193)

  • 0
  • 0
Primary Antibodies

AB182150

Anti-RPB3 antibody [EPR13294(B)]

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPB3 antibody [EPR13294(B)] (AB182150)

  • 0
  • 0

Complementary Products

Primary Antibodies

AB26721

Anti-RNA polymerase II CTD repeat YSPTSPS antibody - ChIP Grade

BOND RX™ Validated

ChIP - Anti-RNA polymerase II CTD repeat YSPTSPS antibody - ChIP Grade (AB26721)

  • 4
  • 13
Primary Antibodies

AB202893

Anti-RPB3 antibody [1Y26]

Western blot - Anti-RPB3 antibody [1Y26] (AB202893)

  • 0
  • 0
Primary Antibodies

AB157099

Anti-RPL5 antibody

Immunoprecipitation - Anti-RPL5 antibody (AB157099)

  • 0
  • 0
Primary Antibodies

AB128995

Anti-RPS3 antibody [EPR7808] - Ribosome Marker

RabMAb|Recombinant|Lab Essentials

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPS3 antibody [EPR7808] - Ribosome Marker (AB128995)

  • 0
  • 0

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com