AB182150

Anti-RPB3 antibody [EPR13294(B)]

RabMAb
Recombinant
What is this?

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(5 Publications)

Rabbit Recombinant Monoclonal RPB3 antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 5 publications.

View Alternative Names

A-152E5.7, POLR2C, DNA-directed RNA polymerase II subunit RPB3, RNA polymerase II subunit 3, RNA polymerase II subunit B3, DNA-directed RNA polymerase II 33 kDa polypeptide, DNA-directed RNA polymerase II subunit C, RPB31, RPB33

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPB3 antibody [EPR13294(B)] (AB182150)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPB3 antibody [EPR13294(B)] (AB182150)

Immunohistochemical analysis of paraffin-embedded Human ovarian carcinoma tissue labeling RPB3 with ab182150 at 1/100 dilution followed by pre-diluted HRP-conjugated secondary antibody and counter-stained with Hematoxylin.

Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

Supplier Data

Western blot - Anti-RPB3 antibody [EPR13294(B)] (AB182150)

All lanes:

Western blot - Anti-RPB3 antibody [EPR13294(B)] (ab182150) at 1/50000 dilution

Lane 1:

Jurkat cell lysate at 20 µg

Lane 2:

HeLa cell lysate at 20 µg

Lane 3:

293T cell lysate at 20 µg

Lane 4:

Human fetal kidney lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

Predicted band size: 31 kDa

false

Carrier free

Anti-RPB3 antibody [EPR13294(B)] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR13294(B)

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IHC-P, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000 - 1/50000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purity

Tissue culture supernatant

Storage buffer

pH: 7.2 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Core component of RNA polymerase II (Pol II), a DNA-dependent RNA polymerase which synthesizes mRNA precursors and many functional non-coding RNAs using the four ribonucleoside triphosphates as substrates.

See full target information POLR2C

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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Cells 14: PubMed39791720

2025

Induction of SUSD2 by STAT3 Activation Is Associated with Tumor Recurrence in HER2-Positive Breast Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Yisun Jeong,Hyungjoo Kim,Daeun You,Soo Youn Cho,Sun Young Yoon,Seok Won Kim,Seok Jin Nam,Jeong Eon Lee,Sangmin Kim

The Korean journal of physiology & pharmacology : official journal of the Korean Physiological Society and the Korean Society of Pharmacology 26:183-193 PubMed35477546

2022

Differentially expressed mRNAs and their upstream miR-491-5p in patients with coronary atherosclerosis as well as the function of miR-491-5p in vascular smooth muscle cells.

Applications

Unspecified application

Species

Unspecified reactive species

Hui Ding,Quanhua Pan,Long Qian,Chuanxian Hu

Molecular cell 81:4413-4424.e5 PubMed34480849

2021

SPT5 stabilization of promoter-proximal RNA polymerase II.

Applications

Unspecified application

Species

Unspecified reactive species

Yuki Aoi,Yoh-Hei Takahashi,Avani P Shah,Marta Iwanaszko,Emily J Rendleman,Nabiha H Khan,Byoung-Kyu Cho,Young Ah Goo,Sheetal Ganesan,Neil L Kelleher,Ali Shilatifard

eLife 10: PubMed34085923

2021

Cytoplasmic mRNA decay represses RNA polymerase II transcription during early apoptosis.

Applications

Unspecified application

Species

Unspecified reactive species

Christopher Duncan-Lewis,Ella Hartenian,Valeria King,Britt A Glaunsinger

eLife 8: PubMed31385803

2019

Tyr1 phosphorylation promotes phosphorylation of Ser2 on the C-terminal domain of eukaryotic RNA polymerase II by P-TEFb.

Applications

Unspecified application

Species

Unspecified reactive species

Joshua E Mayfield,Seema Irani,Edwin E Escobar,Zhao Zhang,Nathaniel T Burkholder,Michelle R Robinson,M Rachel Mehaffey,Sarah N Sipe,Wanjie Yang,Nicholas A Prescott,Karan R Kathuria,Zhijie Liu,Jennifer S Brodbelt,Yan Zhang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-RPB3 antibody [EPR13294(B)]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPB3 antibody [EPR13294(B)] (AB182150)

Western blot - Anti-RPB3 antibody [EPR13294(B)] (AB182150)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purity

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (5)

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