AB128891

Anti-RPE antibody [EPR7464]

RabMAb
Recombinant
KO Validated
What is this?

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(2 Publications)

Rabbit Recombinant Monoclonal RPE antibody. Suitable for WB and reacts with Human samples. Cited in 2 publications.

View Alternative Names

HUSSY-17, RPE, Ribulose-phosphate 3-epimerase, Ribulose-5-phosphate-3-epimerase

2 Images

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Western blot - Anti-RPE antibody [EPR7464] (AB128891)

All lanes:

Western blot - Anti-RPE antibody [EPR7464] (ab128891) at 1/1000 dilution

Lane 1:

K562 cell lysate at 10 µg

Lane 2:

HeLa cell lysate at 10 µg

Lane 3:

HepG2 cell lysate at 10 µg

Lane 4:

293T cell lysate at 10 µg

Secondary

All lanes:

HRP-conjugated goat anti-rabbit at 1/2000 dilution

Predicted band size: 25 kDa

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Lab

Western blot - Anti-RPE antibody [EPR7464] (AB128891)

False colour image of Western blot : Anti-RPE antibody [EPR7464] staining at 1/1000 dilution, shown in green; Mouse anti-Alpha Tubulin [DM1A] (ab7291) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab128891 was shown to bind specifically to RPE. A band was observed at 25 kDa in wild-type HEK-293T cell lysates with no signal observed at this size in rpe knockout cell line ab266325 (knockout cell lysate ab258174). To generate this image, wild-type and rpe knockout HEK-293T cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3% milk in TBS-0.1 % Tween^® 20 (TBS-T) before incubation with primary antibodies overnight at 4°C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preabsorbed (ab216776) at 1/20000 dilution.

All lanes:

Western blot - Anti-RPE antibody [EPR7464] (ab128891) at 1/1000 dilution

Lane 1:

Wild-type HEK-293T cell lysate at 20 µg

Lane 2:

RPE knockout HEK-293T cell lysate at 20 µg

Lane 2:

Western blot - Human RPE knockout HEK-293T cell line (<a href='/en-us/products/cell-lines/human-rpe-knockout-hek-293t-cell-line-ab266325'>ab266325</a>)

Lane 3:

HepG2 cell lysate at 10 µg

Lane 4:

A431 cell lysate at 20 µg

Predicted band size: 25 kDa

Observed band size: 25 kDa

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Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR7464

Isotype

IgG

Carrier free

Reacts with

Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Stable for 12 months at -20°C

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Catalyzes the reversible epimerization of D-ribulose 5-phosphate to D-xylulose 5-phosphate.

See full target information RPE

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

The EMBO journal 43:2636-2660 PubMed38778156

2024

The immune response to RNA suppresses nucleic acid synthesis by limiting ribose 5-phosphate.

Applications

Unspecified application

Species

Unspecified reactive species

Pushpak Bhattacharjee,Die Wang,Dovile Anderson,Joshua N Buckler,Eveline de Geus,Feng Yan,Galina Polekhina,Ralf Schittenhelm,Darren J Creek,Lawrence D Harris,Anthony J Sadler

Oncology letters 17:555-563 PubMed30655801

2019

NANOGP8 expression regulates gastric cancer cell progression by transactivating DBC1 in gastric cancer MKN-45 cells.

Applications

Unspecified application

Species

Unspecified reactive species

Li Li,Ru Feng,Sujuan Fei,Jiang Cao,Qinqin Zhu,Guozhong Ji,Jianwei Zhou

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-RPE antibody [EPR7464]

Western blot - Anti-RPE antibody [EPR7464] (AB128891)

Western blot - Anti-RPE antibody [EPR7464] (AB128891)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Product protocols

Target data

Publications (2)

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Product promise