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AB248202

Anti-RPE antibody [EPR7464] - BSA and Azide free

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Rabbit Recombinant Monoclonal RPE antibody. Carrier free. Suitable for WB and reacts with Human samples.

View Alternative Names

HUSSY-17, RPE, Ribulose-phosphate 3-epimerase, Ribulose-5-phosphate-3-epimerase

2 Images
Western blot - Anti-RPE antibody [EPR7464] - BSA and Azide free (AB248202)
  • WB

Lab

Western blot - Anti-RPE antibody [EPR7464] - BSA and Azide free (AB248202)

False colour image of Western blot : Anti-RPE antibody [EPR7464] staining at 1/1000 dilution, shown in green; Mouse anti-Alpha Tubulin [DM1A] (ab7291) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab128891 was shown to bind specifically to RPE. A band was observed at 25 kDa in wild-type HEK-293T cell lysates with no signal observed at this size in rpe knockout cell line ab266325 (knockout cell lysate ab258174). To generate this image, wild-type and rpe knockout HEK-293T cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) at 1/20000 dilution.

All lanes:

Western blot - Anti-RPE antibody [EPR7464] (<a href='/en-us/products/primary-antibodies/rpe-antibody-epr7464-ab128891'>ab128891</a>) at 1/1000 dilution

Lane 1:

Wild-type HEK-293T cell lysate at 20 µg

Lane 2:

RPE knockout HEK-293T cell lysate at 20 µg

Lane 2:

Western blot - Human RPE knockout HEK-293T cell line (<a href='/en-us/products/cell-lines/human-rpe-knockout-hek-293t-cell-line-ab266325'>ab266325</a>)

Lane 3:

HepG2 cell lysate at 10 µg

Lane 4:

A431 cell lysate at 20 µg

Predicted band size: 25 kDa

Observed band size: 25 kDa

false

Western blot - Anti-RPE antibody [EPR7464] - BSA and Azide free (AB248202)
  • WB

Unknown

Western blot - Anti-RPE antibody [EPR7464] - BSA and Azide free (AB248202)

This data was developed using ab128891, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-RPE antibody [EPR7464] (<a href='/en-us/products/primary-antibodies/rpe-antibody-epr7464-ab128891'>ab128891</a>) at 1/1000 dilution

Lane 1:

K562 cell lysate at 10 µg

Lane 2:

HeLa cell lysate at 10 µg

Lane 3:

HepG2 cell lysate at 10 µg

Lane 4:

293T cell lysate at 10 µg

Secondary

All lanes:

HRP-conjugated goat anti-rabbit at 1/2000 dilution

Predicted band size: 25 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR7464

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Product details

ab248202 is the carrier-free version of ab128891.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Catalyzes the reversible epimerization of D-ribulose 5-phosphate to D-xylulose 5-phosphate.
See full target information RPE
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com