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AB235950

Anti-RPE65 antibody

4

(1 Review)

|

(6 Publications)

Rabbit Polyclonal RPE65 antibody. Suitable for WB, ICC/IF and reacts with Rat, Human samples. Cited in 6 publications. Immunogen corresponding to Recombinant Fragment Protein within Human RPE65 aa 300-500.

View Alternative Names

Retinoid isomerohydrolase, All-trans-retinyl-palmitate hydrolase, Lutein isomerase, Meso-zeaxanthin isomerase, Retinal pigment epithelium-specific 65 kDa protein, Retinol isomerase, RPE65

1 Images
Immunocytochemistry/ Immunofluorescence - Anti-RPE65 antibody (AB235950)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-RPE65 antibody (AB235950)

4% formaldehyde-fixed, 0.2% Triton X-100 permeabilized HeLa (human epithelial cell line from cervix adenocarcinoma) cells labeling RPE65 (green) using ab235950 at 1/233 dilution in ICC/IF. Counterstained with DAPI (blue).

Cells were blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor® 488-conjugated Goat Anti-Rabbit IgG (H+L).

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Rat, Human

Applications

WB, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human RPE65 aa 300-500. The exact immunogen used to generate this antibody is proprietary information.

Q16518

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50 - 1/200", "ICCIF-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/5000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "African green monkey": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Dog": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Purification notes
Purity >95%.
Storage buffer
pH: 7.4 Preservative: 0.03% Proclin 300 Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Critical isomerohydrolase in the retinoid cycle involved in regeneration of 11-cis-retinal, the chromophore of rod and cone opsins. Catalyzes the cleavage and isomerization of all-trans-retinyl fatty acid esters to 11-cis-retinol which is further oxidized by 11-cis retinol dehydrogenase to 11-cis-retinal for use as visual chromophore (PubMed : 16116091). Essential for the production of 11-cis retinal for both rod and cone photoreceptors (PubMed : 17848510). Also capable of catalyzing the isomerization of lutein to meso-zeaxanthin an eye-specific carotenoid (PubMed : 28874556). The soluble form binds vitamin A (all-trans-retinol), making it available for LRAT processing to all-trans-retinyl ester. The membrane form, palmitoylated by LRAT, binds all-trans-retinyl esters, making them available for IMH (isomerohydrolase) processing to all-cis-retinol. The soluble form is regenerated by transferring its palmitoyl groups onto 11-cis-retinol, a reaction catalyzed by LRAT (By similarity).
See full target information RPE65
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Publications (6)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 15:27417 PubMed40721855

2025

Unveiling the impact of OVOL1 on prognosis, immune microenvironment, and proliferation in pancreatic cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Jinping Li,Zhenyan Deng,Zhaofang Yan,Shuxian Huang,Jiamin Jin,Chichu Xie,Jinfeng Gan

Eye and vision (London, England) 10:34 PubMed37528478

2023

Ex vivo cultivated retinal pigment epithelial cell transplantation for the treatment of rabbit corneal endothelial dysfunction.

Applications

Unspecified application

Species

Unspecified reactive species

Chunxiao Dong,Dulei Zou,Haoyun Duan,Xiangyue Hu,Qingjun Zhou,Weiyun Shi,Zongyi Li

Nature communications 13:4233 PubMed35882847

2022

Transcriptomic and proteomic retinal pigment epithelium signatures of age-related macular degeneration.

Applications

Unspecified application

Species

Unspecified reactive species

Anne Senabouth,Maciej Daniszewski,Grace E Lidgerwood,Helena H Liang,Damián Hernández,Mehdi Mirzaei,Stacey N Keenan,Ran Zhang,Xikun Han,Drew Neavin,Louise Rooney,Maria Isabel G Lopez Sanchez,Lerna Gulluyan,Joao A Paulo,Linda Clarke,Lisa S Kearns,Vikkitharan Gnanasambandapillai,Chia-Ling Chan,Uyen Nguyen,Angela M Steinmann,Rachael A McCloy,Nona Farbehi,Vivek K Gupta,David A Mackey,Guy Bylsma,Nitin Verma,Stuart MacGregor,Matthew J Watt,Robyn H Guymer,Joseph E Powell,Alex W Hewitt,Alice Pébay

Frontiers in medicine 9:861371 PubMed35492354

2022

Chaperonin-Containing TCP1 Subunit 5 Protects Against the Effect of Mer Receptor Tyrosine Kinase Knockdown in Retinal Pigment Epithelial Cells by Interacting With Filamentous Actin and Activating the LIM-Kinase 1/Cofilin Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Lujia Feng,Haichun Li,Yong Du,Ting Zhang,Yingting Zhu,Zhidong Li,Ling Zhao,Xing Wang,Gongpei Wang,Linbin Zhou,Zhaorong Jiang,Zheng Liu,Zhancong Ou,Yuwen Wen,Yehong Zhuo

Toxicology reports 9:670-678 PubMed35433274

2022

The effect of docetaxel on retinal pigment epithelial cells.

Applications

Unspecified application

Species

Unspecified reactive species

Jialin Li,Xiao Liu,Qiaohui Yang,Jian Huang,Wang Zhou,Zhirong Tan,Zhuo Li,Di Zhou

Annals of translational medicine 9:245 PubMed33708872

2021

Transplantation of GMP-grade human iPSC-derived retinal pigment epithelial cells in rodent model: the first pre-clinical study for safety and efficacy in China.

Applications

Unspecified application

Species

Unspecified reactive species

Hang Zhang,Bingnan Su,Luyan Jiao,Ze-Hua Xu,Chang-Jun Zhang,Jinfu Nie,Mei-Ling Gao,Ying V Zhang,Zi-Bing Jin
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
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