Rabbit Recombinant Monoclonal RPE65 antibody. Carrier free. Suitable for IP, WB and reacts with Mouse, Human, Rat samples.
Images
![Immunoprecipitation - Anti-RPE65 antibody [EPR7024(N)] - BSA and Azide free (AB249901), expandable thumbnail](https://content.abcam.com/products/images/rpe65-antibody-epr7024n-bsa-and-azide-free-ab249901--immunoprecipitation-img77729.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-RPE65 antibody [EPR7024(N)] - BSA and Azide free (AB249901)")
![Western blot - Anti-RPE65 antibody [EPR7024(N)] - BSA and Azide free (AB249901), expandable thumbnail](https://content.abcam.com/products/images/rpe65-antibody-epr7024n-bsa-and-azide-free-ab249901--western-blot-img66074.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-RPE65 antibody [EPR7024(N)] - BSA and Azide free (AB249901)")
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
Liquid
- Clonality
Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | WB | IHC-P | ICC/IF | |
|---|---|---|---|---|
| Human | Expected | Tested | Not recommended | Not recommended |
| Mouse | Tested | Tested | Not recommended | Not recommended |
| Rat | Expected | Tested | Not recommended | Not recommended |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Human, Rat | Dilution info - | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
Target data
Function
Critical isomerohydrolase in the retinoid cycle involved in regeneration of 11-cis-retinal, the chromophore of rod and cone opsins. Catalyzes the cleavage and isomerization of all-trans-retinyl fatty acid esters to 11-cis-retinol which is further oxidized by 11-cis retinol dehydrogenase to 11-cis-retinal for use as visual chromophore (PubMed:16116091). Essential for the production of 11-cis retinal for both rod and cone photoreceptors (PubMed:17848510). Also capable of catalyzing the isomerization of lutein to meso-zeaxanthin an eye-specific carotenoid (PubMed:28874556). The soluble form binds vitamin A (all-trans-retinol), making it available for LRAT processing to all-trans-retinyl ester. The membrane form, palmitoylated by LRAT, binds all-trans-retinyl esters, making them available for IMH (isomerohydrolase) processing to all-cis-retinol. The soluble form is regenerated by transferring its palmitoyl groups onto 11-cis-retinol, a reaction catalyzed by LRAT (By similarity).
Alternative names
Retinoid isomerohydrolase, All-trans-retinyl-palmitate hydrolase, Lutein isomerase, Meso-zeaxanthin isomerase, Retinal pigment epithelium-specific 65 kDa protein, Retinol isomerase, RPE65
Recommended products
Rabbit Recombinant Monoclonal RPE65 antibody. Carrier free. Suitable for IP, WB and reacts with Mouse, Human, Rat samples.
Alternative names
Retinoid isomerohydrolase, All-trans-retinyl-palmitate hydrolase, Lutein isomerase, Meso-zeaxanthin isomerase, Retinal pigment epithelium-specific 65 kDa protein, Retinol isomerase, RPE65
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
Liquid
- Clonality
Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
Yes
- Clone number
EPR7024(N)
- Purification technique
Affinity purification
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate long-term storage conditions
+4°C
- Storage information
Do Not Freeze
Notes
ab249901 is the carrier-free version of Anti-RPE65 antibody [EPR7024(N)] - C-terminal ab175936.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
![Immunoprecipitation - Anti-RPE65 antibody [EPR7024(N)] - BSA and Azide free (ab249901), expandable thumbnail](https://content.abcam.com/products/images/rpe65-antibody-epr7024n-bsa-and-azide-free-ab249901--immunoprecipitation-img77729.jpg "Immunoprecipitation - Anti-RPE65 antibody [EPR7024(N)] - BSA and Azide free (ab249901)")
Immunoprecipitation - Anti-RPE65 antibody [EPR7024(N)] - BSA and Azide free (ab249901)
This data was developed using Anti-RPE65 antibody [EPR7024(N)] - C-terminal ab175936, the same antibody clone in a different buffer formulation.Western blot analysis on immunoprecipitation pellet from mouse eyeball lysate labeling RPE65 with Anti-RPE65 antibody [EPR7024(N)] - C-terminal ab175936 at 1/10 dilution
All lanes: Immunoprecipitation - Anti-RPE65 antibody [EPR7024(N)] - C-terminal (Anti-RPE65 antibody [EPR7024(N)] - C-terminal ab175936)
Predicted band size: 60 kDa
![Western blot - Anti-RPE65 antibody [EPR7024(N)] - BSA and Azide free (ab249901), expandable thumbnail](https://content.abcam.com/products/images/rpe65-antibody-epr7024n-bsa-and-azide-free-ab249901--western-blot-img66074.jpg "Western blot - Anti-RPE65 antibody [EPR7024(N)] - BSA and Azide free (ab249901)")
Western blot - Anti-RPE65 antibody [EPR7024(N)] - BSA and Azide free (ab249901)
This data was developed using Anti-RPE65 antibody [EPR7024(N)] - C-terminal ab175936, the same antibody clone in a different buffer formulation.
All lanes: Western blot - Anti-RPE65 antibody [EPR7024(N)] - C-terminal (Anti-RPE65 antibody [EPR7024(N)] - C-terminal ab175936) at 1/1000 dilution
Lane 1: Mouse eyeball lysate at 10 µg
Lane 2: Rat eyeball lysate at 10 µg
Lane 3: Y79 lysate at 10 µg
Predicted band size: 60 kDa
Downloads
Product protocols
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