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AB284831

Anti-RPF2 antibody [EP10252] - BSA and Azide free

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Rabbit Monoclonal RPF2 antibody. Carrier free. Suitable for WB, IHC-P, ICC/IF and reacts with Human samples.

View Alternative Names

BXDC1, RPF2, Ribosome production factor 2 homolog, Brix domain-containing protein 1, Ribosome biogenesis protein RPF2 homolog

3 Images
Immunocytochemistry/ Immunofluorescence - Anti-RPF2 antibody [EP10252] - BSA and Azide free (AB284831)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-RPF2 antibody [EP10252] - BSA and Azide free (AB284831)

This data was developed using ab180604, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of 4% paraformaldehyde-fixed 293T cells labeling RPF2 with ab180604 at 1/100 dilution, followed by Goat anti rabbit IgG (Alexa Fluor® 555) at 1/200 dilution (left panel). Dapi stain (right panel).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPF2 antibody [EP10252] - BSA and Azide free (AB284831)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPF2 antibody [EP10252] - BSA and Azide free (AB284831)

This data was developed using ab180604, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human kidney cell carcinoma labeling RPF2 with ab180604 at 1/50 dilution, followed by prediluted ImmunoHistoprobe (Ready to use) HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.

Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

Western blot - Anti-RPF2 antibody [EP10252] - BSA and Azide free (AB284831)
  • WB

Supplier Data

Western blot - Anti-RPF2 antibody [EP10252] - BSA and Azide free (AB284831)

This data was developed using ab180604, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-RPF2 antibody [EP10252] (<a href='/en-us/products/primary-antibodies/rpf2-antibody-ep10252-ab180604'>ab180604</a>) at 1/20000 dilution

Lane 1:

293T cell lysate at 20 µg

Lane 2:

HeLa cell lysate at 20 µg

Lane 3:

A549 cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

Predicted band size: 36 kDa

Observed band size: 36 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EP10252

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB, ICC/IF, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" } } }
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Product details

ab284831 is the carrier-free version of ab180604

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purity
Tissue culture supernatant
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Involved in ribosomal large subunit assembly. May regulate the localization of the 5S RNP/5S ribonucleoprotein particle to the nucleolus.
See full target information RPF2
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com