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Rabbit Recombinant Monoclonal RPL11 antibody. Suitable for WB, IP and reacts with Human, Mouse, Rat samples.

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Images

Western blot - Anti-RPL11 antibody [EPR28901-64] (AB318976), expandable thumbnail
  • Western blot - Anti-RPL11 antibody [EPR28901-64] (AB318976), expandable thumbnail
  • Immunoprecipitation - Anti-RPL11 antibody [EPR28901-64] (AB318976), expandable thumbnail
  • Western blot - Anti-RPL11 antibody [EPR28901-64] (AB318976), expandable thumbnail
  • Immunoprecipitation - Anti-RPL11 antibody [EPR28901-64] (AB318976), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBIPIHC-P
Human
Tested
Tested
Not recommended
Mouse
Tested
Tested
Not recommended
Rat
Tested
Expected
Not recommended

Tested
Tested

Species
Human
Dilution info
1/1000
Notes

-

Species
Mouse
Dilution info
1/1000
Notes

-

Species
Rat
Dilution info
1/1000
Notes

-

Tested
Tested

Species
Human
Dilution info
1/30
Notes

-

Species
Mouse
Dilution info
1/30
Notes

-

Expected
Expected

Species
Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Target data

Function

Component of the ribosome, a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell (PubMed:19191325, PubMed:32669547). The small ribosomal subunit (SSU) binds messenger RNAs (mRNAs) and translates the encoded message by selecting cognate aminoacyl-transfer RNA (tRNA) molecules (PubMed:19191325, PubMed:32669547). The large subunit (LSU) contains the ribosomal catalytic site termed the peptidyl transferase center (PTC), which catalyzes the formation of peptide bonds, thereby polymerizing the amino acids delivered by tRNAs into a polypeptide chain (PubMed:19191325, PubMed:32669547). The nascent polypeptides leave the ribosome through a tunnel in the LSU and interact with protein factors that function in enzymatic processing, targeting, and the membrane insertion of nascent chains at the exit of the ribosomal tunnel (PubMed:19191325, PubMed:32669547). As part of the 5S RNP/5S ribonucleoprotein particle it is an essential component of the LSU, required for its formation and the maturation of rRNAs (PubMed:12962325, PubMed:19061985, PubMed:24120868). It also couples ribosome biogenesis to p53/TP53 activation. As part of the 5S RNP it accumulates in the nucleoplasm and inhibits MDM2, when ribosome biogenesis is perturbed, mediating the stabilization and the activation of TP53 (PubMed:24120868). Promotes nucleolar location of PML (By similarity).

Alternative names

Large ribosomal subunit protein uL5, 60S ribosomal protein L11, CLL-associated antigen KW-12, RPL11

Recommended products

Rabbit Recombinant Monoclonal RPL11 antibody. Suitable for WB, IP and reacts with Human, Mouse, Rat samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR28901-64
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

5 product images

  • Western blot - Anti-RPL11 antibody [EPR28901-64] (ab318976), expandable thumbnail

    Western blot - Anti-RPL11 antibody [EPR28901-64] (ab318976)

    Low expression: brain, cerebellum.

    Lanes 1-3 are applied with Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 and lanes 4-8 are applied with Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000.

    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

    Exposure time: Lanes 1-3: 37 seconds; Lanes 4-8: 26 seconds.

    All lanes: Western blot - Anti-RPL11 antibody [EPR28901-64] (ab318976) at 1/1000 dilution

    Lane 1: Human liver cancer tissue lysate at 20 µg with NFDM/TBST

    Lane 2: Human cerebellum tissue lysate at 20 µg with NFDM/TBST

    Lane 3: Human pancreas tissue lysate at 20 µg with NFDM/TBST

    Lane 4: Mouse liver tissue lysate at 20 µg with NFDM/TBST

    Lane 5: Mouse brain tissue lysate at 20 µg with NFDM/TBST

    Lane 6: Mouse pancreas tissue lysate at 20 µg with NFDM/TBST

    Lane 7: Rat liver tissue lysate at 20 µg with NFDM/TBST

    Lane 8: Rat pancreas tissue lysate at 20 µg with NFDM/TBST

    Secondary

    Lanes 1 - 3: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Lanes 4 - 8: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Observed band size: 18 kDa, 36 kDa

  • Western blot - Anti-RPL11 antibody [EPR28901-64] (ab318976), expandable thumbnail

    Western blot - Anti-RPL11 antibody [EPR28901-64] (ab318976)

    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

    All lanes: Western blot - Anti-RPL11 antibody [EPR28901-64] (ab318976) at 1/1000 dilution

    Lane 1: HeLa (human cervical adenocarcinoma epithelial cell) transfected with scrambled siRNA control whole cell lysate at 10 µg with NFDM/TBST

    Lane 2: HeLa transfected with siRNA specifically targeting RPL11 whole cell lysate at 10 µg with NFDM/TBST

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Observed band size: 18 kDa, 36 kDa

    Exposure time: 15s

  • Immunoprecipitation - Anti-RPL11 antibody [EPR28901-64] (ab318976), expandable thumbnail

    Immunoprecipitation - Anti-RPL11 antibody [EPR28901-64] (ab318976)

    RPL11 was immunoprecipitated from 0.35 mg HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate with ab318976 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab318976 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.

    Lane 1: HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate

    Lane 2: ab318976 IP in HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate

    Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab318976 in HeLa whole cell lysate

    All lanes: Immunoprecipitation - Anti-RPL11 antibody [EPR28901-64] (ab318976) at 1/30 dilution

    All lanes: HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate with NFDM/TBST

    Secondary

    All lanes: Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/5000 dilution

    Exposure time: 10s

  • Western blot - Anti-RPL11 antibody [EPR28901-64] (ab318976), expandable thumbnail

    Western blot - Anti-RPL11 antibody [EPR28901-64] (ab318976)

    All lanes: Western blot - Anti-RPL11 antibody [EPR28901-64] (ab318976) at 1/1000 dilution

    Lane 1: HepG2 (human hepatocellar carcinoma epithelial cell) whole cell lysate at 20 µg with NFDM/TBST

    Lane 2: Jurkat (human T cell leukemia T lymphocyte from peripheral blood) whole cell lysate at 20 µg with NFDM/TBST

    Lane 3: BxPC-3 (human pancreas adenocarcinoma epithelial cell) whole cell lysate at 20 µg with NFDM/TBST

    Lane 4: A549 (human lung carcinoma epithelial cell) whole cell lysate at 20 µg with NFDM/TBST

    Lane 5: NIH/3T3 (mouse embryonic fibroblast) whole cell lysate at 20 µg with NFDM/TBST

    Lane 6: C6 (rat glial tumor glial cell) whole cell lysate at 20 µg with NFDM/TBST

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Observed band size: 18 kDa

    Exposure time: 26s

  • Immunoprecipitation - Anti-RPL11 antibody [EPR28901-64] (ab318976), expandable thumbnail

    Immunoprecipitation - Anti-RPL11 antibody [EPR28901-64] (ab318976)

    RPL11 was immunoprecipitated from 0.35 mg Mouse liver tissue lysate with ab318976 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab318976 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.

    Lane 1: Mouse liver tissue lysate 10ug

    Lane 2: ab318976 IP in Mouse liver tissue lysate

    Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab318976 in mouse liver tissue lysate

    All lanes: Immunoprecipitation - Anti-RPL11 antibody [EPR28901-64] (ab318976) at 1/30 dilution

    All lanes: Mouse liver tissue lysate 10ug with NFDM/TBST

    Secondary

    All lanes: Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/5000 dilution

    Exposure time: 10s

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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