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AB157130

Anti-RPL12 antibody

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(3 Publications)

Rabbit Polyclonal RPL12 antibody. Suitable for WB and reacts with Human, Mouse samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human 60S ribosomal protein L12 aa 100-200.

View Alternative Names

Large ribosomal subunit protein uL11, 60S ribosomal protein L12, RPL12

1 Images
Western blot - Anti-RPL12 antibody (AB157130)
  • WB

Unknown

Western blot - Anti-RPL12 antibody (AB157130)

All lanes:

Western blot - Anti-RPL12 antibody (ab157130) at 0.4 µg/mL

Lane 1:

Jurkat whole cell lysate at 50 µg

Lane 2:

Jurkat whole cell lysate at 15 µg

Lane 3:

HeLa whole cell lysate at 50 µg

Lane 4:

293T whole cell lysate at 50 µg

Lane 5:

NIH3T3 whole cell lysate at 50 µg

Predicted band size: 18 kDa

true

Exposure time: 3min

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

WB

applications

Immunogen

Recombinant Fragment Protein within Human 60S ribosomal protein L12 aa 100-200. The exact immunogen used to generate this antibody is proprietary information.

P30050

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Alternative Products

Primary Antibodies

AB127533

Anti-RPL12 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPL12 antibody (AB127533)

  • 0
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Complementary Products

Primary Antibodies

AB215724

Anti-RPL21 antibody - N-terminal

Western blot - Anti-RPL21 antibody - N-terminal (AB215724)

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Primary Antibodies

AB175213

Anti-Ribosomal protein S11/RPS11 antibody [EPR11487]

RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Anti-Ribosomal protein S11/RPS11 antibody [EPR11487] (AB175213)

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Primary Antibodies

AB176333

Anti-KDEL antibody [EPR12668]

RabMAb|Recombinant|20ul selling size

Immunocytochemistry/ Immunofluorescence - Anti-KDEL antibody [EPR12668] (AB176333)

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Primary Antibodies

AB157108

Anti-hUPF2/RENT2 antibody

Immunoprecipitation - Anti-hUPF2/RENT2 antibody (AB157108)

  • 1
  • 1
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chimpanzee": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chinese hamster": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Dog": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Gorilla": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Lizard": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Pig": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rabbit": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rhesus monkey": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Xenopus laevis": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Xenopus tropicalis": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: 99% Tris citrate/phosphate
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Component of the large ribosomal subunit (PubMed : 25901680). The ribosome is a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell (PubMed : 25901680). Binds directly to 26S ribosomal RNA (PubMed : 25901680).
See full target information 60S ribosomal protein L12
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 12:4358 PubMed34272367

2021

A small molecule that induces translational readthrough of CFTR nonsense mutations by eRF1 depletion.

Applications

Unspecified application

Species

Unspecified reactive species

Jyoti Sharma,Ming Du,Eric Wong,Venkateshwar Mutyam,Yao Li,Jianguo Chen,Jamie Wangen,Kari Thrasher,Lianwu Fu,Ning Peng,Liping Tang,Kaimao Liu,Bini Mathew,Robert J Bostwick,Corinne E Augelli-Szafran,Hermann Bihler,Feng Liang,Jerome Mahiou,Josef Saltz,Andras Rab,Jeong Hong,Eric J Sorscher,Eric M Mendenhall,Candice J Coppola,Kim M Keeling,Rachel Green,Martin Mense,Mark J Suto,Steven M Rowe,David M Bedwell

The Journal of clinical investigation 129:5236-5253 PubMed31657788

2019

Slowing ribosome velocity restores folding and function of mutant CFTR.

Applications

Unspecified application

Species

Unspecified reactive species

Kathryn E Oliver,Robert Rauscher,Marjolein Mijnders,Wei Wang,Matthew J Wolpert,Jessica Maya,Carleen M Sabusap,Robert A Kesterson,Kevin L Kirk,Andras Rab,Ineke Braakman,Jeong S Hong,John L Hartman,Zoya Ignatova,Eric J Sorscher

PLoS biology 14:e1002462 PubMed27168400

2016

Ribosomal Stalk Protein Silencing Partially Corrects the ΔF508-CFTR Functional Expression Defect.

Applications

WB

Species

Human

Guido Veit,Kathryn Oliver,Pirjo M Apaja,Doranda Perdomo,Aurélien Bidaud-Meynard,Sheng-Ting Lin,Jingyu Guo,Mert Icyuz,Eric J Sorscher,John L Hartman,Gergely L Lukacs
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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