AB134961

Anti-RPL13 antibody [EPR8828]

RabMAb
Recombinant
What is this?

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(5 Publications)

Rabbit Recombinant Monoclonal RPL13 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 5 publications.

View Alternative Names

BBC1, OK/SW-cl.46, RPL13, Large ribosomal subunit protein eL13, 60S ribosomal protein L13, Breast basic conserved protein 1

4 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPL13 antibody [EPR8828] (AB134961)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPL13 antibody [EPR8828] (AB134961)

Immunohistochemical analysis of paraffin-embedded Human kidney tissue labelling RPL13 with ab134961 at 1/250 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPL13 antibody [EPR8828] (AB134961)

Immunohistochemical analysis of paraffin-embedded Human thyroid gland adenocarcinoma tissue labelling RPL13 with ab134961 at 1/250 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunocytochemistry/ Immunofluorescence - Anti-RPL13 antibody [EPR8828] (AB134961)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-RPL13 antibody [EPR8828] (AB134961)

Immunofluorescent analysis of MCF7 cells labelling RPL13 with ab134961 at 1/250 dilution.

Unknown

Western blot - Anti-RPL13 antibody [EPR8828] (AB134961)

All lanes:

Western blot - Anti-RPL13 antibody [EPR8828] (ab134961) at 1/1000 dilution

Lane 1:

HeLa cell lysate at 10 µg

Lane 2:

Jurkat cell lysate at 10 µg

Lane 3:

K562 cell lysate at 10 µg

Lane 4:

MCF7 cell lysate at 10 µg

Secondary

All lanes:

HRP labeled goat anti-rabbit at 1/2000 dilution

Predicted band size: 24 kDa

false

Carrier free

Anti-RPL13 antibody [EPR8828] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR8828

Isotype

IgG

Carrier free

Reacts with

Human

Applications

ICC/IF, WB, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/250 - 1/500", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/250 - 1/500", "ICCIF-species-notes": "" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Component of the ribosome, a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell (PubMed : 23636399, PubMed : 31630789, PubMed : 32669547). The small ribosomal subunit (SSU) binds messenger RNAs (mRNAs) and translates the encoded message by selecting cognate aminoacyl-transfer RNA (tRNA) molecules (Probable). The large subunit (LSU) contains the ribosomal catalytic site termed the peptidyl transferase center (PTC), which catalyzes the formation of peptide bonds, thereby polymerizing the amino acids delivered by tRNAs into a polypeptide chain (Probable). The nascent polypeptides leave the ribosome through a tunnel in the LSU and interact with protein factors that function in enzymatic processing, targeting, and the membrane insertion of nascent chains at the exit of the ribosomal tunnel (Probable). As part of the LSU, it is probably required for its formation and the maturation of rRNAs (PubMed : 31630789). Plays a role in bone development (PubMed : 31630789).

See full target information RPL13

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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Journal of pineal research 75:e12897 PubMed37391878

2023

Amelioration of gamma irradiation-induced salivary gland damage in mice using melatonin.

Applications

Unspecified application

Species

Unspecified reactive species

Jingming Ren,Tong Zhu,Zhouxuan Wang,Lu Lu,Saijun Fan

Frontiers in oncology 11:779567 PubMed34993140

2021

Quantitative Proteomic Analysis of Plasma Exosomes to Identify the Candidate Biomarker of Imatinib Resistance in Chronic Myeloid Leukemia Patients.

Applications

Unspecified application

Species

Unspecified reactive species

Mei-Yong Li,Cui Zhao,Lian Chen,Fang-Yi Yao,Fang-Min Zhong,Ying Chen,Shuai Xu,Jun-Yao Jiang,Yu-Lin Yang,Qing-Hua Min,Jin Lin,Hai-Bin Zhang,Jing Liu,Xiao-Zhong Wang,Bo Huang

Molecular cell 81:1425-1438.e10 PubMed33662272

2021

PQBP1 promotes translational elongation and regulates hippocampal mGluR-LTD by suppressing eEF2 phosphorylation.

Applications

Unspecified application

Species

Unspecified reactive species

Yuqian Shen,Zi Chao Zhang,Shanshan Cheng,An Liu,Jian Zuo,Shuting Xia,Xian Liu,Wenhua Liu,Zhengping Jia,Wei Xie,Junhai Han

Frontiers in cell and developmental biology 9:618899 PubMed33644057

2021

Cisplatin-Resistant Gastric Cancer Cells Promote the Chemoresistance of Cisplatin-Sensitive Cells via the Exosomal RPS3-Mediated PI3K-Akt-Cofilin-1 Signaling Axis.

Applications

Unspecified application

Species

Unspecified reactive species

Meng-Yao Sun,Bo Xu,Qiu-Xue Wu,Wen-Lian Chen,Si Cai,Hui Zhang,Qing-Feng Tang

The Journal of cell biology 207:213-23 PubMed25349259

2014

CLUH regulates mitochondrial biogenesis by binding mRNAs of nuclear-encoded mitochondrial proteins.

Applications

Unspecified application

Species

Unspecified reactive species

Jie Gao,Désirée Schatton,Paola Martinelli,Henriette Hansen,David Pla-Martin,Esther Barth,Christian Becker,Janine Altmueller,Peter Frommolt,Marco Sardiello,Elena I Rugarli

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-RPL13 antibody [EPR8828]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPL13 antibody [EPR8828] (AB134961)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPL13 antibody [EPR8828] (AB134961)

Immunocytochemistry/ Immunofluorescence - Anti-RPL13 antibody [EPR8828] (AB134961)

Western blot - Anti-RPL13 antibody [EPR8828] (AB134961)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Product protocols

Target data

Publications (5)

Product promise