AB181200

Anti-RPL14 antibody [EPR12301]

RabMAb
Recombinant
What is this?

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(7 Publications)

Rabbit Recombinant Monoclonal RPL14 antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 7 publications.

View Alternative Names

Large ribosomal subunit protein eL14, 60S ribosomal protein L14, CAG-ISL 7, RPL14

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPL14 antibody [EPR12301] (AB181200)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPL14 antibody [EPR12301] (AB181200)

Immunohistochemical analysis of paraffin-embedded Human heart muscle tissue labeling RPL14 with ab181200 at 1/250 dilution followed by pre-diluted HRP-conjugated secondary antibody and counter-stained with Hematoxylin.

Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPL14 antibody [EPR12301] (AB181200)

Immunohistochemical analysis of paraffin-embedded Human cardiac muscle tissue labeling RPL14 with ab181200 at 1/250 dilution followed by pre-diluted HRP-conjugated secondary antibody and counter-stained with Hematoxylin.

Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

Supplier Data

Western blot - Anti-RPL14 antibody [EPR12301] (AB181200)

All lanes:

Western blot - Anti-RPL14 antibody [EPR12301] (ab181200) at 1/10000 dilution

Lane 1:

HeLa cell lysate at 20 µg

Lane 2:

293 cell lysate at 20 µg

Lane 3:

Jurkat cell lysate at 20 µg

Lane 4:

MCF7 cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 23 kDa

false

Carrier free

Anti-RPL14 antibody [EPR12301] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR12301

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IHC-P, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/250", "IHCP-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The RPL14 protein also known as ribosomal protein L14 plays an important role in the ribosomal 60S subunit which is essential for protein synthesis. This protein has a molecular weight of around 15 kDa and is primarily expressed in the cytoplasm. As a component of the ribosome RPL14 contributes to the structure and stability of ribosomal RNA facilitating the translation process.

Biological function summary

Ribosomal protein L14 integrates into the large ribosomal subunit participating in the assembly of the ribosome complex. RPL14 binds to 28S rRNA and contributes to the ribosome’s ability to accurately synthesize proteins. It works alongside other ribosomal proteins and RNA to ensure efficient translation of messenger RNA (mRNA) into proteins impacting cell growth and proliferation.

Pathways

RPL14 has significant involvement in the translation pathway critical for genetic expression and protein synthesis. It interacts with other ribosomal proteins within the translation machinery such as RPL5 and RPL11 to facilitate the binding of the aminoacyl-tRNA to the ribosome. In the context of the mTOR signaling pathway RPL14 potentially influences cell growth and metabolism by regulating protein synthesis rates.

The dysfunction of ribosomal protein L14 associates with certain ribosomopathies which are disorders caused by ribosomal dysfunction. Mutations or altered expression of RPL14 can also relate to cancer where deregulated protein synthesis contributes to uncontrolled cell proliferation. The interaction of RPL14 with other ribosomal proteins like RPL11 suggests its involvement in the maintenance of genomic integrity further linking it to disease states characterized by disrupted cellular homeostasis.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Component of the large ribosomal subunit (PubMed : 12962325, PubMed : 23636399, PubMed : 32669547). The ribosome is a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell (PubMed : 12962325, PubMed : 23636399, PubMed : 32669547).

See full target information RPL14

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Publications (7)

Recent publications for all applications. Explore the full list and refine your search

Journal of gastrointestinal oncology 16:191-208 PubMed40115921

2025

PRMT5 attenuates regorafenib-induced DNA damage in hepatocellular carcinoma cells through symmetric dimethylation of RPL14.

Applications

Unspecified application

Species

Unspecified reactive species

Wendi Bi,Xiaojuan Sun,Qiuyun Yi,Xinyu Jiang,Huisi He,Lixuan Jiang,Zhecai Fan,Hailing Huang,Wen Wen,Xiaoqing Jiang

Frontiers in microbiology 15:1452739 PubMed39749135

2025

Limitations of a proper SFTSV mouse model using human C-type lectin receptors.

Applications

Unspecified application

Species

Unspecified reactive species

You-Min Kim,Hyo-Jin Ro,Jae Hoon Lee,Yaechan Song,Han-Woong Lee,Nam-Hyuk Cho

The Journal of cell biology 224: PubMed39602297

2024

Identification of coilin interactors reveals coordinated control of Cajal body number and structure.

Applications

Unspecified application

Species

Unspecified reactive species

Dahyana Arias Escayola,Chuyue Zhang,Emily Nischwitz,Leonard Schärfen,Kerstin Dörner,Korinna Straube,Ulrike Kutay,Falk Butter,Karla M Neugebauer

Journal of pineal research 75:e12897 PubMed37391878

2023

Amelioration of gamma irradiation-induced salivary gland damage in mice using melatonin.

Applications

Unspecified application

Species

Unspecified reactive species

Jingming Ren,Tong Zhu,Zhouxuan Wang,Lu Lu,Saijun Fan

Cell cycle (Georgetown, Tex.) 22:986-1004 PubMed36762777

2023

Metformin attenuates multiple myeloma cell proliferation and encourages apoptosis by suppressing METTL3-mediated m6A methylation of THRAP3, RBM25, and USP4.

Applications

Unspecified application

Species

Unspecified reactive species

Cong-Jie Chen,Jie-Yun Huang,Jian-Qing Huang,Jia-Yi Deng,Xiao-Hui Shangguan,Ai-Zhen Chen,Long-Tian Chen,Wei-Hao Wu

Frontiers in oncology 11:779567 PubMed34993140

2021

Quantitative Proteomic Analysis of Plasma Exosomes to Identify the Candidate Biomarker of Imatinib Resistance in Chronic Myeloid Leukemia Patients.

Applications

Unspecified application

Species

Unspecified reactive species

Mei-Yong Li,Cui Zhao,Lian Chen,Fang-Yi Yao,Fang-Min Zhong,Ying Chen,Shuai Xu,Jun-Yao Jiang,Yu-Lin Yang,Qing-Hua Min,Jin Lin,Hai-Bin Zhang,Jing Liu,Xiao-Zhong Wang,Bo Huang

OncoTargets and therapy 14:551-564 PubMed33505161

2021

Propofol Inhibits the Progression of Cervical Cancer by Regulating HOTAIR/miR-129-5p/RPL14 Axis.

Applications

Unspecified application

Species

Unspecified reactive species

Nai Sun,Wei Zhang,Jiaying Liu,Xiaochen Yang,Qinjun Chu

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-RPL14 antibody [EPR12301]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPL14 antibody [EPR12301] (AB181200)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPL14 antibody [EPR12301] (AB181200)

Western blot - Anti-RPL14 antibody [EPR12301] (AB181200)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (7)

Product promise