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AB250422

Anti-RPL14 antibody [EPR12301] - BSA and Azide free

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Rabbit Recombinant Monoclonal RPL14 antibody. Carrier free. Suitable for WB, IHC-P and reacts with Human samples.

View Alternative Names

Large ribosomal subunit protein eL14, 60S ribosomal protein L14, CAG-ISL 7, RPL14

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPL14 antibody [EPR12301] - BSA and Azide free (AB250422)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPL14 antibody [EPR12301] - BSA and Azide free (AB250422)

This data was developed using ab181200, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human heart muscle tissue labeling RPL14 with ab181200 at 1/250 dilution followed by pre-diluted HRP-conjugated secondary antibody and counter-stained with Hematoxylin.

Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPL14 antibody [EPR12301] - BSA and Azide free (AB250422)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPL14 antibody [EPR12301] - BSA and Azide free (AB250422)

This data was developed using ab181200, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human cardiac muscle tissue labeling RPL14 with ab181200 at 1/250 dilution followed by pre-diluted HRP-conjugated secondary antibody and counter-stained with Hematoxylin.

Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

Western blot - Anti-RPL14 antibody [EPR12301] - BSA and Azide free (AB250422)
  • WB

Supplier Data

Western blot - Anti-RPL14 antibody [EPR12301] - BSA and Azide free (AB250422)

This data was developed using ab181200, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-RPL14 antibody [EPR12301] (<a href='/en-us/products/primary-antibodies/rpl14-antibody-epr12301-ab181200'>ab181200</a>) at 1/10000 dilution

Lane 1:

HeLa cell lysate at 20 µg

Lane 2:

293 cell lysate at 20 µg

Lane 3:

Jurkat cell lysate at 20 µg

Lane 4:

MCF7 cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 23 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR12301

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Product details

ab250422 is the carrier-free version of ab181200.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The RPL14 protein also known as ribosomal protein L14 plays an important role in the ribosomal 60S subunit which is essential for protein synthesis. This protein has a molecular weight of around 15 kDa and is primarily expressed in the cytoplasm. As a component of the ribosome RPL14 contributes to the structure and stability of ribosomal RNA facilitating the translation process.
Biological function summary

Ribosomal protein L14 integrates into the large ribosomal subunit participating in the assembly of the ribosome complex. RPL14 binds to 28S rRNA and contributes to the ribosome’s ability to accurately synthesize proteins. It works alongside other ribosomal proteins and RNA to ensure efficient translation of messenger RNA (mRNA) into proteins impacting cell growth and proliferation.

Pathways

RPL14 has significant involvement in the translation pathway critical for genetic expression and protein synthesis. It interacts with other ribosomal proteins within the translation machinery such as RPL5 and RPL11 to facilitate the binding of the aminoacyl-tRNA to the ribosome. In the context of the mTOR signaling pathway RPL14 potentially influences cell growth and metabolism by regulating protein synthesis rates.

The dysfunction of ribosomal protein L14 associates with certain ribosomopathies which are disorders caused by ribosomal dysfunction. Mutations or altered expression of RPL14 can also relate to cancer where deregulated protein synthesis contributes to uncontrolled cell proliferation. The interaction of RPL14 with other ribosomal proteins like RPL11 suggests its involvement in the maintenance of genomic integrity further linking it to disease states characterized by disrupted cellular homeostasis.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Component of the large ribosomal subunit (PubMed : 12962325, PubMed : 23636399, PubMed : 32669547). The ribosome is a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell (PubMed : 12962325, PubMed : 23636399, PubMed : 32669547).
See full target information RPL14
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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