AB155802

Anti-RPL15/L10 antibody

Name: Anti-RPL15/L10 antibody (ab155802) | Abcam
Brand: Abcam Limited
SKU: AB155802
Price: 535 USD
Availability: InStock

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(5 Publications)

Rabbit Polyclonal RPL15/L10 antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 5 publications. Immunogen corresponding to Recombinant Fragment Protein within Human RPL15 aa 1-200.

View Alternative Names

EC45, TCBAP0781, RPL15, Large ribosomal subunit protein eL15, 60S ribosomal protein L15

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPL15/L10 antibody (AB155802)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPL15/L10 antibody (AB155802)

Immunohistochemical analysis of paraffin-embedded Normal Colon tissue labeling RPL15/L10 with ab155802 at 1/500 dilution.

Unknown

Western blot - Anti-RPL15/L10 antibody (AB155802)

12% SDS PAGE

All lanes:

Western blot - Anti-RPL15/L10 antibody (ab155802) at 1/1000 dilution

All lanes:

H1299 whole cell lysate at 30 µg

Predicted band size: 24 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human RPL15 aa 1-200. The exact immunogen used to generate this antibody is proprietary information.

P61313

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p>Use 10mM Citrate buffer or alternatively Tris-EDTA buffer (pH 8.0) may be used</p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Monkey": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Pig": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Xenopus laevis": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Xenopus tropicalis": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Zebrafish": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: PBS, 20% Glycerol (glycerin, glycerine), 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

RPL15 also known as ribosomal protein L15 or L10 is involved in protein synthesis as part of the ribosomal subunit. It has a mass of approximately 24 kDa. RPL15 is expressed in various tissues showing high levels in rapidly dividing cells such as those in the bone marrow and some cancer tissues. As a component of the large 60S ribosomal subunit it directly participates in the assembly and function of ribosomes.

Biological function summary

RPL15 acts in concert with other ribosomal proteins to ensure proper translation of mRNA into proteins. It is part of the ribosome a complex and essential structure for cellular function and growth. The ribosome consists of RNA and proteins where RPL15 plays a role in maintaining the integrity and functionality of this complex. Without RPL15 cells would experience impaired protein synthesis which can significantly impact cell viability and proliferation.

Pathways

RPL15 is significant in the mRNA translation pathway a core process involved in generating cellular proteins. It connects with various other ribosomal proteins such as RPL6 and RPL13 to ensure accurate and efficient translation. Another pathway where RPL15 has a role includes the signal recognition particle (SRP)-dependent co-translational protein targeting to membrane which directs protein synthesis to specific cellular locations illustrating its integration into cellular machinery.

RPL15 has shown a connection to cancer particularly in its overexpression in certain tumors where its high levels can drive increased protein synthesis fostering rapid tumor growth. Furthermore RPL15's relationship with ribosomal protein S6 (RPS6) another key player in protein synthesis highlights its involvement in pathways that can lead to ribosomal biogenesis disorders a group of diseases often related to cell growth and division abnormalities like certain anemias.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Component of the large ribosomal subunit. The ribosome is a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell.

See full target information RPL15

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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Cell reports. Medicine 5:101552 PubMed38729158

2024

Inhibition of protein translational machinery in triple-negative breast cancer as a promising therapeutic strategy.

Applications

Unspecified application

Species

Unspecified reactive species

Arpit Dheeraj,Fernando Jose Garcia Marques,Dhanir Tailor,Abel Bermudez,Angel Resendez,Mallesh Pandrala,Benedikt Grau,Praveen Kumar,Carrsyn B Haley,Alexander Honkala,Praveen Kujur,Stefanie S Jeffrey,Sharon Pitteri,Sanjay V Malhotra

Cancer cell international 22:150 PubMed35410346

2022

RPL15 promotes hepatocellular carcinoma progression via regulation of RPs-MDM2-p53 signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Rui Shi,Zirong Liu

Frontiers in molecular neuroscience 14:688050 PubMed34630032

2021

Tandem Mass Tag-Based Quantitative Proteomic Analysis Reveals Pathways Involved in Brain Injury Induced by Chest Exposure to Shock Waves.

Applications

Unspecified application

Species

Unspecified reactive species

Changci Tong,Peifang Cong,Ying Liu,Xiuyun Shi,Lin Shi,Shun Mao,Yan Zhao,Mingxiao Hou,Yunen Liu

International journal of molecular sciences 22: PubMed34068231

2021

eIF3a Destabilization and TDP-43 Alter Dynamics of Heat-Induced Stress Granules.

Applications

Unspecified application

Species

Unspecified reactive species

Ivana Malcova,Lenka Senohrabkova,Lenka Novakova,Jiri Hasek

Nanoscale 11:3344-3359 PubMed30724952

2019

Polyethyleneimine-assisted one-pot synthesis of quasi-fractal plasmonic gold nanocomposites as a photothermal theranostic agent.

Applications

Unspecified application

Species

Unspecified reactive species

Vladimir Mulens-Arias,Alba Nicolás-Boluda,Alexandre Gehanno,Alice Balfourier,Florent Carn,Florence Gazeau

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-RPL15/L10 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPL15/L10 antibody (AB155802)

Western blot - Anti-RPL15/L10 antibody (AB155802)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (5)

Product promise