Rabbit Polyclonal RPL15/L10 antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 4 publications. Immunogen corresponding to Recombinant Fragment Protein within Human 60S ribosomal protein L15 aa 1-200.
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Publications
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- Cancer cell international 22:1502022RPL15 promotes hepatocellular carcinoma progression via regulation of RPs-MDM2-p53 signaling pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRui Shi,Zirong LiuPubMed 35410346
- Frontiers in molecular neuroscience 14:6880502021Tandem Mass Tag-Based Quantitative Proteomic Analysis Reveals Pathways Involved in Brain Injury Induced by Chest Exposure to Shock Waves.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChangci Tong,Peifang Cong,Ying Liu,Xiuyun Shi,Lin Shi,Shun Mao,Yan Zhao,Mingxiao Hou,Yunen LiuPubMed 34630032
- International journal of molecular sciences 22:2021eIF3a Destabilization and TDP-43 Alter Dynamics of Heat-Induced Stress Granules.Applications:Unspecified applicationReactive species:Unspecified reactive speciesIvana Malcova,Lenka Senohrabkova,Lenka Novakova,Jiri HasekPubMed 34068231
- Nanoscale 11:3344-33592019Polyethyleneimine-assisted one-pot synthesis of quasi-fractal plasmonic gold nanocomposites as a photothermal theranostic agent.Applications:Unspecified applicationReactive species:Unspecified reactive speciesVladimir Mulens-Arias,Alba Nicolás-Boluda,Alexandre Gehanno,Alice Balfourier,Florent Carn,Florence GazeauPubMed 30724952
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 78.99% PBS, 20% Glycerol (glycerin, glycerine), 1% BSA- Form
Liquid
- Clonality
Polyclonal
Immunogen
- Recombinant Fragment Protein within Human 60S ribosomal protein L15 aa 1-200. The exact immunogen used to generate this antibody is proprietary information. Database link P61313
Reactivity data
Select an application| WB | IHC-P | |
|---|---|---|
| Human | Tested | Tested |
| Mouse | Predicted | Predicted |
| Rat | Predicted | Predicted |
| Cow | Predicted | Predicted |
| Monkey | Predicted | Predicted |
| Pig | Predicted | Predicted |
| Xenopus laevis | Predicted | Predicted |
| Xenopus tropicalis | Predicted | Predicted |
| Zebrafish | Predicted | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500.00000 - 1/3000.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Cow, Pig, Xenopus laevis, Monkey, Zebrafish, Xenopus tropicalis | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Use 10mM Citrate buffer or alternatively Tris-EDTA buffer (pH 8.0) may be used |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Cow, Pig, Xenopus laevis, Monkey, Zebrafish, Xenopus tropicalis | Dilution info - | Notes - |
Target data
Function
Component of the large ribosomal subunit. The ribosome is a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell.
Alternative names
EC45, TCBAP0781, RPL15, EC45, TCBAP0781, Large ribosomal subunit protein eL15, 60S ribosomal protein L15
Recommended products
Rabbit Polyclonal RPL15/L10 antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 4 publications. Immunogen corresponding to Recombinant Fragment Protein within Human 60S ribosomal protein L15 aa 1-200.
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 78.99% PBS, 20% Glycerol (glycerin, glycerine), 1% BSA- Form
Liquid
- Clonality
Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human 60S ribosomal protein L15 aa 1-200. The exact immunogen used to generate this antibody is proprietary information. Database link P61313
- Purification technique
Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
RPL15 also known as ribosomal protein L15 or L10 is involved in protein synthesis as part of the ribosomal subunit. It has a mass of approximately 24 kDa. RPL15 is expressed in various tissues showing high levels in rapidly dividing cells such as those in the bone marrow and some cancer tissues. As a component of the large 60S ribosomal subunit it directly participates in the assembly and function of ribosomes.
- Biological function summary
RPL15 acts in concert with other ribosomal proteins to ensure proper translation of mRNA into proteins. It is part of the ribosome a complex and essential structure for cellular function and growth. The ribosome consists of RNA and proteins where RPL15 plays a role in maintaining the integrity and functionality of this complex. Without RPL15 cells would experience impaired protein synthesis which can significantly impact cell viability and proliferation.
- Pathways
RPL15 is significant in the mRNA translation pathway a core process involved in generating cellular proteins. It connects with various other ribosomal proteins such as RPL6 and RPL13 to ensure accurate and efficient translation. Another pathway where RPL15 has a role includes the signal recognition particle (SRP)-dependent co-translational protein targeting to membrane which directs protein synthesis to specific cellular locations illustrating its integration into cellular machinery.
- Associated diseases and disorders
RPL15 has shown a connection to cancer particularly in its overexpression in certain tumors where its high levels can drive increased protein synthesis fostering rapid tumor growth. Furthermore RPL15's relationship with ribosomal protein S6 (RPS6) another key player in protein synthesis highlights its involvement in pathways that can lead to ribosomal biogenesis disorders a group of diseases often related to cell growth and division abnormalities like certain anemias.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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2 product images
Western blot - Anti-RPL15/L10 antibody (ab155802)
12% SDS PAGE
All lanes: Western blot - Anti-RPL15/L10 antibody (ab155802) at 1/1000 dilution
All lanes: H1299 whole cell lysate at 30 µg
Predicted band size: 24 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPL15/L10 antibody (ab155802)
Immunohistochemical analysis of paraffin-embedded Normal Colon tissue labeling RPL15/L10 with ab155802 at 1/500 dilution.
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