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AB155781

Anti-RPL17 antibody

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(4 Publications )

Rabbit Polyclonal RPL17 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Rat, Human samples. Cited in 4 publications. Immunogen corresponding to Recombinant Fragment Protein within Human RPL17 aa 1 to C-terminus.
4 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPL17 antibody (AB155781)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPL17 antibody (AB155781)

Immunohistochemical analysis of paraffin-embedded Human HBL435 xenograft, labeling RPL17 with ab155781 at 1/500 dilution.

Immunocytochemistry/ Immunofluorescence - Anti-RPL17 antibody (AB155781)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-RPL17 antibody (AB155781)

Immunofluorescence analysis of HeLa cells fixed in 4% paraformaldehyde at RT for 15 min labelling RPL17 protein at cytoplasm using ab155781 at a 1/500 dilution (Green).
alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody at a 1/10000 dilution (Red). Hoechst 33342 was used for nuclear staining (Blue).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPL17 antibody (AB155781)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPL17 antibody (AB155781)

Immunohistochemical analysis of rat liver tissue stained for RPL17 protein at cytoplasm with ab155781 at a 1/500 dilution.

Antigen retrieval : Citrate buffer, pH 6.0, 15 min.

Western blot - Anti-RPL17 antibody (AB155781)
  • WB

Supplier Data

Western blot - Anti-RPL17 antibody (AB155781)

Samples were separated by 12% SDS PAGE.

All lanes:

Western blot - Anti-RPL17 antibody (ab155781) at 1/1000 dilution

All lanes:

Rat pancreas tissue extract at 50 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 97 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Rat, Human

Applications

WB, IHC-P, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human RPL17 aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P18621

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/500 - 1/3000", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Monkey": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Pig": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Xenopus tropicalis": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

RPL17 also known as Ribosomal Protein L17 is an essential component of the ribosome the cellular machinery responsible for protein synthesis. It is encoded by the RPL17 gene and its protein product has a mass of approximately 21 kDa. Expressed in various tissues RPL17 shows high levels in cells with active protein production needs including in liver kidney and heart tissues.
Biological function summary

RPL17 acts as a part of the ribosomal 60S subunit playing a role in assembling and stabilizing the ribosome structure. It interacts closely with other ribosomal proteins and ribosomal RNA (rRNA) to facilitate efficient protein translation. As a structural constituent of the ribosome it does not work in isolation but as part of a larger complex necessary for mRNA translation into polypeptides.

Pathways

The protein RPL17 is involved in the translation pathway and ribosome biogenesis. These pathways are important for gene expression. Within the translation pathway RPL17 interacts with multiple ribosomal proteins such as RPL5 and RPL10 to ensure accurate decoding of mRNA. The protein's integration aids in the assembly and function of the ribosome which impacts overall cellular function and growth.

Alterations or defects in the expression of RPL17 have been linked to ribosomopathies which are disorders characterized by defective ribosome function. For example Diamond-Blackfan anemia involves mutations in ribosomal proteins including RPL17 which disrupt normal erythropoiesis. Additionally RPL17 may interact with tumor suppressor proteins like p53 implicating it in pathways related to cancer development and progression. Understanding these connections furthers insights into the molecular basis of these conditions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Component of the large ribosomal subunit (PubMed : 12962325, PubMed : 23636399, PubMed : 32669547). The ribosome is a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell (PubMed : 12962325, PubMed : 23636399, PubMed : 32669547).
See full target information RPL17

Alternative Names

Large ribosomal subunit protein uL22, 60S ribosomal protein L17, 60S ribosomal protein L23, PD-1, RPL17
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Publications (4)

Recent publications for all applications. Explore the full listand refine your search

PLoS pathogens 20:e1012766 PubMed39621795

2024

Flaviviruses induce ER-specific remodelling of protein synthesis.

Applications

Unspecified application

Species

Unspecified reactive species

Ho Him Wong,Dorian Richard Kenneth Crudgington,Lewis Siu,Sumana Sanyal

The Journal of clinical investigation 134: PubMed38875287

2024

Liver cancer initiation requires translational activation by an oncofetal regulon involving LIN28 proteins.

Applications

Unspecified application

Species

Unspecified reactive species

Meng-Hsiung Hsieh,Yonglong Wei,Lin Li,Liem H Nguyen,Yu-Hsuan Lin,Jung M Yong,Xuxu Sun,Xun Wang,Xin Luo,Sarah K Knutson,Christina Bracken,George Q Daley,John T Powers,Hao Zhu

Journal of Cancer 13:2570-2583 PubMed35711835

2022

RPL17 Promotes Colorectal Cancer Proliferation and Stemness through ERK and NEK2/β-catenin Signaling Pathways.

Applications

Unspecified application

Species

Unspecified reactive species

Min Ji Ko,Yu-Ri Seo,Daekwan Seo,So-Young Park,Ji Hae Seo,Eun-Hye Jeon,Sang-Woo Kim,Keon Uk Park,Deog-Bon Koo,Shin Kim,Jae-Hoon Bae,Dae-Kyu Song,Chi Heum Cho,Kwang Seok Kim,Yun-Han Lee

Nature communications 8:14124 PubMed28112156

2017

mTORC1 inhibition in cancer cells protects from glutaminolysis-mediated apoptosis during nutrient limitation.

Applications

Unspecified application

Species

Unspecified reactive species

Victor H Villar,Tra Ly Nguyen,Vanessa Delcroix,Silvia Terés,Marion Bouchecareilh,Bénédicte Salin,Clément Bodineau,Pierre Vacher,Muriel Priault,Pierre Soubeyran,Raúl V Durán
View all publications
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Product promise

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