AB241418

Anti-RPL18A antibody

Name: Anti-RPL18A antibody (ab241418) | Abcam
Brand: Abcam Limited
SKU: AB241418
Price: 525 USD
Availability: InStock

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(1 Publication)

Rabbit Polyclonal RPL18A antibody. Suitable for IP, WB and reacts with Human, Mouse samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human RPL18A aa 100 to C-terminus.

View Alternative Names

Large ribosomal subunit protein eL20, 60S ribosomal protein L18a, RPL18A

2 Images

Supplier Data

Immunoprecipitation - Anti-RPL18A antibody (AB241418)

RPL18A was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab241418 at 6 μg/reaction. Western blot was performed from the immunoprecipitate using ab241418 at 0.4 μg/ml.

Lane 1 : ab241418 IP in HEK-293T whole cell lysate.
Lane 2 : Control IgG IP in HEK-293T whole cell lysate.

Detection : Chemiluminescence with exposure time of 30 seconds.

Lysates prepared in NETN buffer.

All lanes:

Immunoprecipitation - Anti-RPL18A antibody (ab241418)

Predicted band size: 21 kDa

false

Supplier Data

Western blot - Anti-RPL18A antibody (AB241418)

Lysates prepared in NETN buffer.

All lanes:

Western blot - Anti-RPL18A antibody (ab241418) at 0.1 µg/mL

Lane 1:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg

Lane 2:

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg

Lane 3:

Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate at 50 µg

Lane 4:

TCMK-1 (mouse kidney epithelial cell line) whole cell lysate at 50 µg

Lane 5:

NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate at 50 µg

Predicted band size: 21 kDa

true

Exposure time: 10s

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human, Mouse

Applications

WB, IP

applications

Immunogen

Synthetic Peptide within Human RPL18A aa 100 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q02543

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-10 µg/mg of lysate", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "" }, "Rat": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Zebrafish": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

Antibody was affinity purified using an epitope specific to RPL18A immobilized on solid support.

Storage buffer

pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: Tris citrate/phosphate

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

RPL18A also known as 60S ribosomal protein L18a plays a part in ribosome structure and function. This ribosomal protein with a molecular mass of approximately 22 kDa is an important part of the large 60S subunit of the ribosome. It is present in various tissues and has ubiquitous expression reflecting its role in protein synthesis. It functions as a structural constituent and it interacts directly with ribosomal RNA to help create the core framework necessary for ribosome stability.

Biological function summary

Ribosomal protein L18a contributes to the assembly and function of ribosomes the essential machines for translation of mRNA into proteins. It is a part of the ribosomal complex which includes numerous proteins and RNA molecules that collaborate for translation. L18a assists in maintaining the proper structure needed for the translation process impacting cell growth and proliferation. Also ribosomal proteins like RPL18A have non-ribosomal roles in regulating cell cycle and apoptosis showing their importance beyond protein synthesis.

Pathways

Ribosome biogenesis and protein synthesis involve RPL18A. It is a component of the ribosomal pathway closely related to other ribosomal proteins like RPL5 and RPL11 essential for the synthesis of new proteins. RPL18A also intersects with the mTOR signaling pathway which senses nutritional and energy conditions to regulate cell growth emphasizing its role in metabolic control.

Mutations or dysregulation of RPL18A connect to conditions such as Diamond-Blackfan anemia a rare genetic disorder resulting in failure to make red blood cells. RPL18A mutation leads to disrupted ribosome biogenesis similarly observed in many cancers where rapid cell growth demands increased protein synthesis. The interaction between RPL18A and other ribosomal proteins like RPL11 suggests their combined influence on the progression of these disorders by altering ribosomal function and cellular homeostasis.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Component of the large ribosomal subunit. The ribosome is a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell.

See full target information RPL18A

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Communications biology 5:564 PubMed35681048

2022

Stabilization of CDK6 by ribosomal protein uS7, a target protein of the natural product fucoxanthinol.

Applications

Unspecified application

Species

Unspecified reactive species

Yosuke Iizumi,Yoshihiro Sowa,Wakana Goi,Yuichi Aono,Motoki Watanabe,Yoichi Kurumida,Tomoshi Kameda,Kenichi Akaji,Masatoshi Kitagawa,Toshiyuki Sakai

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-RPL18A antibody

Immunoprecipitation - Anti-RPL18A antibody (AB241418)

Western blot - Anti-RPL18A antibody (AB241418)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Product promise