AB224592

Anti-RPL19 antibody

Name: Anti-RPL19 antibody (ab224592) | Abcam
Brand: Abcam Limited
SKU: AB224592

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(2 Publications)

Rabbit Polyclonal RPL19 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Mouse, Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human RPL19 aa 50 to C-terminus.

View Alternative Names

Large ribosomal subunit protein eL19, 60S ribosomal protein L19, RPL19

7 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPL19 antibody (AB224592)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPL19 antibody (AB224592)

Paraffin-embedded human brain tissue stained for RPL19 using ab224592 at 1/100 dilution in immunohistochemical analysis.

Immunocytochemistry/ Immunofluorescence - Anti-RPL19 antibody (AB224592)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-RPL19 antibody (AB224592)

HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for RPL19 (green) using ab224592 at 1/100 dilution in ICC/IF.

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-RPL19 antibody (AB224592)

Immunofluorescent analysis of HeLa (human epithelial cell line from cervix adenocarcinoma) cells labelling RPL19 with ab224592 at 1/100 dilution, followed by Alexa Fluor 488^®-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) (green).

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPL19 antibody (AB224592)

Paraffin-embedded human tonsil tissue stained for RPL19 using ab224592 at 1/100 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPL19 antibody (AB224592)

Paraffin-embedded human endometrial cancer tissue stained for RPL19 using ab224592 at 1/100 dilution in immunohistochemical analysis.

Supplier Data

Western blot - Anti-RPL19 antibody (AB224592)

All lanes:

Western blot - Anti-RPL19 antibody (ab224592) at 1/500 dilution

All lanes:

Mouse spleen tissue lysate

Secondary

All lanes:

Goat polyclonal to rabbit IgG at 1/50000 dilution

Predicted band size: 23 kDa

Observed band size: 24 kDa

false

Supplier Data

Western blot - Anti-RPL19 antibody (AB224592)

All lanes:

Western blot - Anti-RPL19 antibody (ab224592) at 1/500 dilution

Lane 1:

Hela whole cell lysate

Lane 2:

MCF-7 whole cell lysate

Lane 3:

293 whole cell lysate

Lane 4:

THP-1 whole cell lysate

Lane 6:

Mouse brain tissue

Secondary

All lanes:

Goat polyclonal to rabbit IgG at 1/50000 dilution

Predicted band size: 23 kDa

Observed band size: 28 kDa,56 kDa

false

Exposure time: 5min

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

ICC/IF, WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human RPL19 aa 50 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P84098

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/20 - 1/200", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50 - 1/200", "ICCIF-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/5000", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Catfish": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cynomolgus monkey": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Dog": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Orangutan": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Xenopus laevis": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Zebrafish": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification

Storage buffer

pH: 7.4 Preservative: 0.03% Proclin 300 Constituents: PBS, 50% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

RPL19 also known as 60S ribosomal protein L19 is a component of the 60S subunit of the ribosome. It has a molecular weight of approximately 23 kDa. This protein is ubiquitously expressed in many tissues reflecting its essential role in protein synthesis. As part of the ribosomal structure RPL19 contributes to the assembly and stability of the ribosome aiding in the accurate translation of mRNA into proteins.

Biological function summary

RPL19 functions within the ribosome which is a complex molecular machine responsible for protein synthesis in cells. This protein plays a role in the ribosomal large subunit assembly and stability. RPL19 is part of the ribonucleoprotein complex and interacts with ribosomal RNA and other ribosomal proteins to ensure correct translation process. Maintaining ribosomal structural integrity and function helps in the cells' overall proteome homeostasis.

Pathways

RPL19 is involved in the translation machinery particularly the mRNA translation and protein synthesis pathway. This protein contributes to the ribosomal large subunit in translational machinery which is central to cellular growth and proliferation. It interacts with several ribosomal proteins including RPL5 and RPL11 which are key components in coordinating the synthesis of proteins necessary for various cellular processes. The NMD (nonsense-mediated decay) pathway also governs the regulation of RPL19 ensuring the fidelity and quality control of the protein synthesis.

RPL19's dysfunction has implications in ribosomopathies and certain cancers. Aberrations in its expression or function can disrupt normal protein synthesis leading to developmental disorders. Furthermore dysregulation of RPL19 is linked to tumorigenesis notably in disorders involving p53 as the ribosomal proteins interact with MDM2 and influence p53 activity affecting cell cycle regulation and apoptosis. Understanding these connections highlights RPL19's potential as a therapeutic target in related conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Component of the large ribosomal subunit (PubMed : 23636399, PubMed : 32669547). The ribosome is a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell (PubMed : 23636399, PubMed : 32669547).

See full target information RPL19

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in pharmacology 16:1588722 PubMed40771929

2025

Dual PI3K/mTOR inhibitor NVP-BEZ235 induces cell cycle arrest via autophagy mediated protein degradation of RPL19 in nephroblastoma cell.

Applications

Unspecified application

Species

Unspecified reactive species

Yan Gao,Xinran Xing,Ruizhi Cai,Dong Liu,Qili Feng,Jiaqi Luo,Yongzhao Zhu,Zeli Su

Diagnostics (Basel, Switzerland) 15: PubMed40506951

2025

The Role of Endocan Expression in the Diagnosis and Grading of Precancerous Gastric Lesions.

Applications

Unspecified application

Species

Unspecified reactive species

Mehmet Kok,Suleyman Dolu,Mehmet Emin Arayici,Gokhan Koker,Kadir Balaban,Ayhan Hilmi Cekin

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-RPL19 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPL19 antibody (AB224592)

Immunocytochemistry/ Immunofluorescence - Anti-RPL19 antibody (AB224592)

Immunocytochemistry/ Immunofluorescence - Anti-RPL19 antibody (AB224592)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPL19 antibody (AB224592)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPL19 antibody (AB224592)

Western blot - Anti-RPL19 antibody (AB224592)

Western blot - Anti-RPL19 antibody (AB224592)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

Product promise