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AB194664

Anti-RPL21 antibody

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(1 Publication)

Mouse Polyclonal RPL21 antibody. Suitable for WB and reacts with Mouse, Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human RPL21 aa 1-100.

View Alternative Names

Large ribosomal subunit protein eL21, 60S ribosomal protein L21, RPL21

4 Images
Western blot - Anti-RPL21 antibody (AB194664)
  • WB

Supplier Data

Western blot - Anti-RPL21 antibody (AB194664)

All lanes:

Western blot - Anti-RPL21 antibody (ab194664) at 1/500 dilution

All lanes:

HepG2 cell lysate at 50 µg

Predicted band size: 19 kDa

true

Western blot - Anti-RPL21 antibody (AB194664)
  • WB

Supplier Data

Western blot - Anti-RPL21 antibody (AB194664)

All lanes:

Western blot - Anti-RPL21 antibody (ab194664) at 1/500 dilution

All lanes:

RAW 264.7 cell lysate at 50 µg

Predicted band size: 19 kDa

true

Western blot - Anti-RPL21 antibody (AB194664)
  • WB

Supplier Data

Western blot - Anti-RPL21 antibody (AB194664)

All lanes:

Western blot - Anti-RPL21 antibody (ab194664) at 1/500 dilution

All lanes:

NIH 3T3 cell lysate at 50 µg

Predicted band size: 19 kDa

false

Western blot - Anti-RPL21 antibody (AB194664)
  • WB

Supplier Data

Western blot - Anti-RPL21 antibody (AB194664)

Predicted MWt of immunogen : 35.35 kDa.

All lanes:

Western blot - Anti-RPL21 antibody (ab194664) at 1/1000 dilution

All lanes:

Recombinant Human RPL21 protein (immunogen) at 0.2 µg

Predicted band size: 19 kDa

true

Key facts

Host species

Mouse

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

WB

applications

Immunogen

Recombinant Fragment Protein within Human RPL21 aa 1-100. The exact immunogen used to generate this antibody is proprietary information.

P46778

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Alternative Products

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Primary Antibodies

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Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

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  • 5
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2500", "WB-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2500", "WB-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Goat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purity
Whole antiserum
Storage buffer
Constituents: 50% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The RPL21 protein also known as 60S ribosomal protein L21 forms part of the ribosomal 60S subunit. This protein weighs approximately 18 kDa. It expresses in various tissues with significant expression in rapidly dividing cells where ribosome production is higher. RPL21 is involved in protein synthesis by contributing to the assembly of ribosomes facilitating the translation of messenger RNA into proteins.
Biological function summary

RPL21 has a significant role in ribosome biogenesis and function. It is a component of the large ribosomal subunit complex which consists of numerous proteins and RNA molecules. This complex translocates along mRNA strands to facilitate the synthesis of polypeptide chains. RPL21's presence in the ribosome is essential for maintaining the structural integrity needed for efficient translation processes.

Pathways

RPL21 acts within the mRNA translation pathway and is important in the ribosome assembly pathway. It interacts directly with other ribosomal proteins such as RPL5 and RPL11 aiding the coordination and assembly of the 60S ribosomal subunit. The ribosome assembly pathway is fundamental in cell growth and division linking RPL21 with regulatory pathways that control protein synthesis rates.

Mutations or dysregulation of RPL21 associate with diamond-blackfan anemia and certain cancers. In diamond-blackfan anemia defective ribosomal proteins like RPL21 lead to impaired ribosome assembly resulting in decreased erythrocyte production. In cancer altered expression of RPL21 may connect with oncogenic pathways where it interacts with proto-oncogenes or tumor suppressors that drive tumorigenesis.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Component of the large ribosomal subunit (PubMed : 12962325, PubMed : 23636399, PubMed : 25901680, PubMed : 25957688). The ribosome is a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell (PubMed : 12962325, PubMed : 23636399, PubMed : 25901680, PubMed : 25957688).
See full target information RPL21
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Molecular medicine reports 18:2561-2570 PubMed30015916

2018

Aucubin suppresses Titanium particles‑mediated apoptosis of MC3T3‑E1 cells and facilitates osteogenesis by affecting the BMP2/Smads/RunX2 signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Ziguan Zhu,Qingping Xie,Yazeng Huang,Shuijun Zhang,Yu Chen
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Product promise

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