AB215724

Anti-RPL21 antibody - N-terminal

Name: Anti-RPL21 antibody - N-terminal (ab215724) | Abcam
Brand: Abcam Limited
SKU: AB215724
Price: 520 USD
Availability: InStock

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(2 Publications)

Rabbit Polyclonal RPL21 antibody. N-terminal. Suitable for WB and reacts with Human, Mouse samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human RPL21 aa 1-50 conjugated to Keyhole Limpet Haemocyanin.

View Alternative Names

Large ribosomal subunit protein eL21, 60S ribosomal protein L21, RPL21

1 Images

Supplier Data

Western blot - Anti-RPL21 antibody - N-terminal (AB215724)

All lanes:

Western blot - Anti-RPL21 antibody - N-terminal (ab215724) at 0.1 µg/mL

Lane 1:

HeLa whole cell lysate at 50 µg

Lane 2:

293T whole cell lysate at 50 µg

Lane 3:

Jurkat whole cell lysate at 50 µg

Lane 4:

TCMK-1 whole cell lysate at 50 µg

Lane 5:

NIH3T3 whole cell lysate at 50 µg

Predicted band size: 19 kDa

true

Exposure time: 10s

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

applications

Immunogen

Synthetic Peptide within Human RPL21 aa 1-50 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.

P46778

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chimpanzee": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Dog": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Goat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Gorilla": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Horse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Pig": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rabbit": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rhesus monkey": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Sheep": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: 99% Tris citrate/phosphate

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The RPL21 protein also known as 60S ribosomal protein L21 forms part of the ribosomal 60S subunit. This protein weighs approximately 18 kDa. It expresses in various tissues with significant expression in rapidly dividing cells where ribosome production is higher. RPL21 is involved in protein synthesis by contributing to the assembly of ribosomes facilitating the translation of messenger RNA into proteins.

Biological function summary

RPL21 has a significant role in ribosome biogenesis and function. It is a component of the large ribosomal subunit complex which consists of numerous proteins and RNA molecules. This complex translocates along mRNA strands to facilitate the synthesis of polypeptide chains. RPL21's presence in the ribosome is essential for maintaining the structural integrity needed for efficient translation processes.

Pathways

RPL21 acts within the mRNA translation pathway and is important in the ribosome assembly pathway. It interacts directly with other ribosomal proteins such as RPL5 and RPL11 aiding the coordination and assembly of the 60S ribosomal subunit. The ribosome assembly pathway is fundamental in cell growth and division linking RPL21 with regulatory pathways that control protein synthesis rates.

Mutations or dysregulation of RPL21 associate with diamond-blackfan anemia and certain cancers. In diamond-blackfan anemia defective ribosomal proteins like RPL21 lead to impaired ribosome assembly resulting in decreased erythrocyte production. In cancer altered expression of RPL21 may connect with oncogenic pathways where it interacts with proto-oncogenes or tumor suppressors that drive tumorigenesis.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Component of the large ribosomal subunit (PubMed : 12962325, PubMed : 23636399, PubMed : 25901680, PubMed : 25957688). The ribosome is a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell (PubMed : 12962325, PubMed : 23636399, PubMed : 25901680, PubMed : 25957688).

See full target information RPL21

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Molecular cell 83:1075-1092.e9 PubMed36868228

2023

DAXX adds a de novo H3.3K9me3 deposition pathway to the histone chaperone network.

Applications

Unspecified application

Species

Unspecified reactive species

Massimo Carraro,Ivo A Hendriks,Colin M Hammond,Victor Solis-Mezarino,Moritz Völker-Albert,Jonas D Elsborg,Melanie B Weisser,Christos Spanos,Guillermo Montoya,Juri Rappsilber,Axel Imhof,Michael L Nielsen,Anja Groth

Cell reports 41:111571 PubMed36323262

2022

Nuclear stabilization of p53 requires a functional nucleolar surveillance pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Katherine M Hannan,Priscilla Soo,Mei S Wong,Justine K Lee,Nadine Hein,Perlita Poh,Kira D Wysoke,Tobias D Williams,Christian Montellese,Lorey K Smith,Sheren J Al-Obaidi,Lorena Núñez-Villacís,Megan Pavy,Jin-Shu He,Kate M Parsons,Karagh E Loring,Tess Morrison,Jeannine Diesch,Gaetan Burgio,Rita Ferreira,Zhi-Ping Feng,Cathryn M Gould,Piyush B Madhamshettiwar,Johan Flygare,Thomas J Gonda,Kaylene J Simpson,Ulrike Kutay,Richard B Pearson,Christoph Engel,Nicholas J Watkins,Ross D Hannan,Amee J George

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-RPL21 antibody - N-terminal

Western blot - Anti-RPL21 antibody - N-terminal (AB215724)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Secondary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

Product promise