AB241088

Anti-RPL23 antibody

Name: Anti-RPL23 antibody (ab241088) | Abcam
Brand: Abcam Limited
SKU: AB241088
Price: 525 USD
Availability: InStock

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(3 Publications)

Rabbit Polyclonal RPL23 antibody. Suitable for IP, WB and reacts with Human, Mouse samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human RPL23 aa 50 to C-terminus.

View Alternative Names

Large ribosomal subunit protein uL14, 60S ribosomal protein L17, 60S ribosomal protein L23, RPL23

2 Images

Supplier Data

Western blot - Anti-RPL23 antibody (AB241088)

Prepared using NETN lysis buffer.

All lanes:

Western blot - Anti-RPL23 antibody (ab241088) at 0.1 µg/mL

Lane 1:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg

Lane 2:

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg

Lane 3:

Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate at 50 µg

Lane 4:

TCMK-1 (Mouse kidney epithelial cell line) whole cell lysate at 50 µg

Lane 5:

NIH/3T3 (Mouse embryo fibroblast cell line) whole cell lysate at 50 µg

Predicted band size: 15 kDa

true

Exposure time: 10s

Supplier Data

Immunoprecipitation - Anti-RPL23 antibody (AB241088)

RPL23 was immunoprecipitated from HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (1 mg per IP reaction, 20% of IP loaded, prepared using NETN lysis buffer) using ab241088 at 6 μg per reaction. Western blot was performed from the immunoprecipitate using ab241088 at 1 μg/mL.

Lane 1 : ab241088 IP in HEK-293T whole cell lysate.
Lane 2 : Control IgG.

Detection : Chemiluminescence with an exposure time of 10 seconds.

All lanes:

Immunoprecipitation - Anti-RPL23 antibody (ab241088)

Predicted band size: 15 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

WB, IP

applications

Immunogen

Synthetic Peptide within Human RPL23 aa 50 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P62829

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-10 µg/mg of lysate", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "" }, "Rat": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Drosophila melanogaster": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Pig": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Zebrafish": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: Tris citrate/phosphate

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

RPL23 also known as ribosomal protein L23 is an essential component of the large 60S ribosomal subunit with a molecular mass of approximately 15 kDa. This protein is highly expressed in eukaryotic cells particularly in tissues with high rates of protein synthesis. RPL23 interacts directly with rRNA to facilitate its structural stability and function playing an important role in the assembly of the ribosome and the translation of mRNA into proteins.

Biological function summary

RPL23 contributes to the synthesis of proteins by forming part of the ribosomal complex. Ribosomes are composed of two subunits 40S and 60S in eukaryotes with RPL23 being integral to the larger subunit. The presence of RPL23 ensures that the ribosomes effectively translate the genetic code carried by mRNA into a polypeptide chain. This protein is essential in maintaining the fidelity and efficiency of translation processes within cells determining how cells grow and respond to various stimuli.

Pathways

RPL23 plays a significant role in the translation initiation pathway and the protein synthesis pathway which are important for cell growth and replication. Within these pathways RPL23 associates with other ribosomal proteins such as RPL5 and RPL11 which together modulate the formation of the ribosomal structure and oversee the translation process. The function of RPL23 in these pathways ensures that cellular resources are properly managed and directed towards productive protein synthesis.

Mutations or dysregulation of RPL23 have connections to cancer and Diamond-Blackfan anemia. The alteration in RPL23 can disrupt normal ribosome function leading to impaired protein synthesis and contributing to the uncontrolled cell growth seen in cancers. This protein also interacts with tumor suppressor proteins like TP53 linking its activity to mechanisms that control cell cycle and apoptosis. Furthermore deficiencies in RPL23 function may lead to insufficient production of red blood cells evident in Diamond-Blackfan anemia highlighting a relationship with genes responsible for balanced erythropoiesis.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Component of the large ribosomal subunit. The ribosome is a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell.

See full target information RPL23

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

NAR cancer 7:zcaf024 PubMed40918646

2025

Loss of ribosomal protein uL14 enables tumor escape from T cell immunosurveillance.

Applications

Unspecified application

Species

Unspecified reactive species

Anna Dopler,Edwin S Kyei-Baffour,Mandy Kerkhoff,Ferhat Alkan,Yuval Malka,Kelly Hoefakker,Rob van der Kammen,Liesbeth Hoekman,Onno Bleijerveld,Antonia Bradaric,Maarten Altelaar,Jonathan W Yewdell,Pia Kvistborg,William J Faller

American journal of medical genetics. Part A 191:2757-2767 PubMed37596828

2023

Novel association of Dandy-Walker malformation with CAPN15 variants expands the phenotype of oculogastrointestinal neurodevelopmental syndrome.

Applications

Unspecified application

Species

Unspecified reactive species

M Makenzie Beaman,Lucia Guidugli,Monia Hammer,Chelsea Barrows,Anne Gregor,Sangmoon Lee,Kristen L Deak,Marie T McDonald,Courtney Jensen,Maha S Zaki,Amira T Masri,Charlotte A Hobbs,Joseph G Gleeson,Jennifer L Cohen

Oncology reports 43:1863-1874 PubMed32236588

2020

Triptolide interrupts rRNA synthesis and induces the RPL23‑MDM2‑p53 pathway to repress lung cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Juan Wang,Zhi-Qian Zhang,Fang-Qiong Li,Jia-Ning Chen,Xiaoting Gong,Bei-Bei Cao,Wei Wang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-RPL23 antibody

Western blot - Anti-RPL23 antibody (AB241088)

Immunoprecipitation - Anti-RPL23 antibody (AB241088)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

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